| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 613,318 | T→A | S724S (TCT→TCA) | SAUSA300_RS02915 → | serine‑aspartate repeat‑containing protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 613,318 | 0 | T | A | 88.9% | 15.1 / ‑4.2 | 9 | S724S (TCT→TCA) | SAUSA300_RS02915 | serine‑aspartate repeat‑containing protein C |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (7/1); minor base G (1/0); total (8/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
GTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGA > NC_007793/613238‑613410 | gTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACtcag > 1:2724978/1‑100 (MQ=255) atgatgATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACtcagatagcgactcagattc < 1:6614854/100‑1 (MQ=255) cacaCTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACtcagatagcgactcagattcagatagcgac > 1:3727239/1‑100 (MQ=255) ctAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCagatagcgactcagattcagatagcgactcagattc > 1:5438779/2‑100 (MQ=255) ggCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAgattcagaca > 1:5487822/1‑100 (MQ=255) cgacgcAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTgatgcggacagcgattcagccacagat > 1:1500651/6‑95 (MQ=0) cAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCgactcaga > 1:1629896/1‑100 (MQ=255) cAGACAGCGATTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAgattcagacagcgattcagaccgcagatcgga > 1:5739114/1‑92 (MQ=11) cAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCga > 1:2081049/1‑100 (MQ=255) | GTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGA > NC_007793/613238‑613410 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |