Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077932,249,0090AT76.9% 15.8 / 2.1 13intergenic (‑396/+3)SAUSA300_RS11480/SAUSA300_RS11485hypothetical protein/ATP‑grasp domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base T (0/10);  total (0/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

TTGACTAGAATCGAAAAAAGCTTGTTACAAACGCATATTCAGTCAACTACTGGCAACACAACATTGTAGCGCCTTATGTTCCGAACTCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCTTGATTTA  >  NC_007793/2248925‑2249105
                                                                                    |                                                                                                
ttGACTAGAATCGAAAAAAGCTTGTTACAAACGCATATTCAGTCAACTACTGGCAACACAACATTGTAGCGCCTTATGTTCCGAACTCTAATAGTGTTCa                                                                                   <  1:1585876/100‑1 (MQ=255)
                                                          caacaTTGTAGCGCCTTATGTTCCGAACTCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGcc                         <  1:4633427/100‑1 (MQ=255)
                                                                    acgtgtgctcttCCGATCTCTAATGGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGcc               <  1:6172541/90‑1 (MQ=255)
                                                                    acgtgtgctcttCCGATCTCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGcc               <  1:1855267/90‑1 (MQ=255)
                                                                    acgtgtgctcttCCGATCTCT‑ATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCa              <  1:325630/90‑1 (MQ=255)
                                                                     cgtgtgctcttCCGATCTCTAGTAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCa              <  1:3951700/91‑1 (MQ=255)
                                                                     cgtgtgctcttCCGATCTCC‑ATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTTCTGTTTCTTTTGAATCACCATGCCAAATACAGCCaa             <  1:6360781/91‑1 (MQ=255)
                                                                           ctcttCCGATCTTT‑ATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCTTg       <  1:6629470/97‑1 (MQ=255)
                                                                           ctcttCCGATCTCTAAAAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCtt        <  1:236476/97‑1 (MQ=255)
                                                                           ccgatctgAACCCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCtt        <  1:931125/93‑1 (MQ=255)
                                                                            tcttCCGATCTCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCAATCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCtgt       <  1:3829429/98‑3 (MQ=255)
                                                                            tcttCCGATCTCAAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCTTg       <  1:5529428/98‑1 (MQ=255)
                                                                                 cGATCTCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCTTGAtttc  <  1:2524548/100‑2 (MQ=255)
                                                                                    |                                                                                                
TTGACTAGAATCGAAAAAAGCTTGTTACAAACGCATATTCAGTCAACTACTGGCAACACAACATTGTAGCGCCTTATGTTCCGAACTCTAATAGTGTTCAAGTTCAGCTAAAAATGCGCGTTCATTCTCCAGTACTGTTTCTTTTGAATCACCATGCCAAATACAGCCAAACCTTGATTTA  >  NC_007793/2248925‑2249105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑