Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793621,8900TG79.7% 198.1 / 267.0 569S999A (TCA→GCA) SAUSA300_RS02925MSCRAMM family adhesin SdrE
Reads supporting (aligned to +/- strand):  ref base T (114/1);  new base G (444/6);  total (561/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-06

Only 100 of 698 total aligned reads displayed.

TTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTC  >  NC_007793/621793‑621981
                                                                                                 |                                                                                           
gccagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgca                                                                                           >  1:5399113/9‑100 (MQ=0)
 tctgatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcag                                                                                          >  1:4336649/10‑100 (MQ=2)
 cttgatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcag                                                                                          >  1:2118038/10‑100 (MQ=2)
  tagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                                                         >  1:1319620/7‑100 (MQ=2)
  ctgatgcggaCAGCGATTCAGACGCGGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                                                         >  1:1772038/9‑100 (MQ=2)
  ctgatgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATtcagacgcaga                                                                                         >  1:6345301/9‑100 (MQ=2)
  ctgatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcaaacgcaga                                                                                         >  1:5958961/9‑100 (MQ=2)
  ctgatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcaga                                                                                         >  1:2937919/9‑100 (MQ=2)
  ctgatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcaga                                                                                         >  1:2250189/9‑100 (MQ=2)
  cagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATtcagactcaga                                                                                         >  1:6125688/1‑100 (MQ=255)
  cagacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                                                         >  1:6290065/7‑100 (MQ=0)
   ccgtgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtctgacgcagat                                                                                        >  1:1443421/8‑99 (MQ=2)
   agattcagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagat                                                                                        >  1:1141375/1‑99 (MQ=11)
   agacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagat                                                                                        >  1:5635279/6‑99 (MQ=0)
   agacgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagat                                                                                        >  1:3371473/6‑99 (MQ=0)
    gatgcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagata                                                                                       >  1:2456790/7‑98 (MQ=2)
    gatgcggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagata                                                                                       >  1:3060999/7‑98 (MQ=2)
    gatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcagata                                                                                       >  1:3622106/7‑98 (MQ=2)
    gatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACtcagacgcagata                                                                                       >  1:5483763/7‑98 (MQ=2)
    gatgcggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGAGTCTGATGCAGACAGCGACtcagacgcagata                                                                                       >  1:904023/7‑98 (MQ=2)
    cacccggaCAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagata                                                                                       >  1:2011649/7‑98 (MQ=2)
     ctgcagaTAGCGATTCAGATTCAGATAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTCTGATGCAGACAg                                                                                      >  1:5738999/4‑100 (MQ=2)
     acgcagaTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacacagatcg                                                                                      >  1:4355021/4‑97 (MQ=2)
      ttcagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagatcgg                                                                                     >  1:2835328/1‑96 (MQ=11)
      tgcagaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATACTGATGCGGACAGc                                                                                     >  1:4077026/3‑100 (MQ=2)
      ctcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGCTTCAGATTCAGATAGCGATTCTGATGCGGACAGc                                                                                     >  1:2517801/2‑100 (MQ=2)
      ctcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGc                                                                                     >  1:4397999/2‑100 (MQ=11)
       tcagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCAGACAGCGATTCTGACGCGGACAGCg                                                                                    >  1:6580672/1‑100 (MQ=2)
       tcagaCAGCGATTCAGACACAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                                                                    >  1:1899575/1‑100 (MQ=11)
       gcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                                                                    >  1:3430254/4‑100 (MQ=2)
       gcggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                                                                    >  1:1270589/4‑100 (MQ=2)
       ccggaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGCTAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCg                                                                                    >  1:6192255/4‑100 (MQ=2)
        cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                                                   >  1:4941180/1‑100 (MQ=11)
        cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                                                   >  1:4721782/1‑100 (MQ=11)
        cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                                                   >  1:1488659/1‑100 (MQ=11)
        cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                                                   >  1:1611886/1‑100 (MQ=11)
        cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                                                   >  1:5108427/1‑100 (MQ=11)
        cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                                                   >  1:6072099/1‑100 (MQ=11)
        cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                                                   >  1:1970135/1‑100 (MQ=11)
        cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                                                   >  1:3273676/1‑100 (MQ=11)
        cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                                                   >  1:6397421/1‑100 (MQ=11)
        cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATGCGGACAGCGa                                                                                   >  1:4675108/1‑100 (MQ=11)
        cagaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATACAGATTCAGATAGCGATTCTGATGCGGACAGCGa                                                                                   >  1:718998/1‑100 (MQ=2)
         tgataGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGat                                                                                  >  1:276907/5‑99 (MQ=0)
         ggaCAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCAGACAGCGACTCAGACGCAGATAGcaat                                                                                  >  1:5323368/2‑97 (MQ=0)
         atgcAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGat                                                                                  >  1:1250653/4‑99 (MQ=2)
         agaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGat                                                                                  >  1:101862/1‑99 (MQ=11)
         agaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGat                                                                                  >  1:2707212/1‑99 (MQ=11)
         agaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGat                                                                                  >  1:582111/1‑99 (MQ=11)
         agaCAGCGACTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATtcaaacgcagatcggaag                                                                                  >  1:380430/1‑93 (MQ=2)
         acccAGCGACTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCCGACAGCGACTCAGACGCAGATAGCGat                                                                                  >  1:503473/4‑99 (MQ=2)
          tgcAGCGATTCAGACGCAGATAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGTCAGCGatt                                                                                 >  1:2876723/3‑98 (MQ=2)
          taCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGatt                                                                                 >  1:515262/2‑98 (MQ=2)
          aaCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGatt                                                                                 >  1:4534807/2‑98 (MQ=2)
           ccAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGAAtc                                                                                >  1:5514970/2‑100 (MQ=2)
           ccAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATGCAGATAGCGATTCTGATGCGGACAGCGATtc                                                                                >  1:521078/2‑100 (MQ=2)
           ataGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGGGATtc                                                                                <  1:3143623/98‑1 (MQ=2)
           aCAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATACGGACAGCGAGtc                                                                                >  1:1393850/1‑100 (MQ=2)
                    cagattcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCACACAGCGATtcagactcaga                                                                       >  1:3888702/1‑100 (MQ=2)
                    cagacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                                       >  1:6434524/7‑100 (MQ=2)
                    acgacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcaga                                                                       >  1:205117/7‑100 (MQ=2)
                     cgacgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATtcagacgcagat                                                                      >  1:6024568/6‑100 (MQ=0)
                     agattcagatAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACtcagatgcagac                                                                      <  1:4223672/100‑2 (MQ=11)
                       ccgcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAg                                                                    >  1:1196543/4‑100 (MQ=2)
                        agcagatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGc                                                                   >  1:1847752/3‑100 (MQ=2)
                          cagatAGCGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGa                                                                 >  1:2647140/1‑100 (MQ=11)
                           agatAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGAt                                                                >  1:936397/1‑100 (MQ=11)
                             atAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTc                                                              <  1:6216200/100‑1 (MQ=11)
                              caGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCCATTct                                                             >  1:1708271/2‑99 (MQ=2)
                               aGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTcag                                                            <  1:4182668/100‑3 (MQ=11)
                                    cTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATTCAGATAGCGAtactgatgcg                                                       >  1:1353123/1‑91 (MQ=2)
                                     tctgATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcgg                                                      >  1:5876539/4‑95 (MQ=2)
                                     tCAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAg                                                      >  1:2410662/1‑100 (MQ=11)
                                      ctgATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGACTCTGatgcgga                                                     >  1:6640119/3‑94 (MQ=0)
                                      ctgATGCGGACAGCGACTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCAGAcgctga                                                     >  1:3847925/3‑95 (MQ=2)
                                      cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTTAGATTCAGATAGCGATTCAGATCCAGACAGCGACTCAGACGCAGATAGCGATTCAGAcgaaga                                                     >  1:804540/1‑95 (MQ=11)
                                      cAGATTCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGa                                                     >  1:375735/1‑100 (MQ=11)
                                        gATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGGTTCAGACGCAGATAGCGATTCAGATTCAGATAGCGACTCTGatgcggaca                                                   >  1:568645/1‑92 (MQ=0)
                                        gATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGatgcggaca                                                   >  1:3467086/1‑92 (MQ=0)
                                        gATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggaca                                                   >  1:3546660/1‑92 (MQ=2)
                                        gATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggaca                                                   >  1:400562/1‑92 (MQ=2)
                                        gATGCGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGatgcggaca                                                   >  1:4129588/1‑92 (MQ=2)
                                        gATGCGGACAGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGCTTCAGACGCAGTTAGCGATTCAGATTCAGATAGCGATTCTGatgcggaca                                                   >  1:5441170/1‑92 (MQ=0)
                                         ttgcGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGAAAGCGATTCAGATTCAGATAGAGATTCTGATGCGGACAg                                                  >  1:3923908/4‑100 (MQ=2)
                                         ctgcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAg                                                  >  1:3005421/4‑100 (MQ=2)
                                         atgcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAg                                                  >  1:1075677/4‑100 (MQ=2)
                                          gtcagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGCTAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGc                                                 >  1:4804179/5‑100 (MQ=1)
                                           tcagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCg                                                >  1:5286802/4‑100 (MQ=1)
                                           tcagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCg                                                >  1:231901/4‑100 (MQ=1)
                                           gcGGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGACGCAGACAGCg                                                >  1:3218359/2‑100 (MQ=11)
                                           ccagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCg                                                >  1:4497253/4‑100 (MQ=11)
                                            cagACAGCGATTCAGACGCAGATATCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                               >  1:6512541/3‑100 (MQ=11)
                                            cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCGGACGCAGATAGCGa                                               >  1:4866607/3‑100 (MQ=11)
                                            cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                               >  1:2556260/3‑100 (MQ=11)
                                            cagACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGa                                               >  1:1104702/3‑100 (MQ=11)
                                            cGGACAGCGATTCAGACGCAGATAGCGATTCAGATCCAGATAGCGACTCTGATGCGGACAGCGACTCAGATTCAGACAGCGATTCGGACGCAGatcggaa                                               >  1:3103734/1‑94 (MQ=11)
                                             tgACAGCGATTCAGACGCAGATAGCGATTCAGGTTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGac                                              >  1:164874/2‑99 (MQ=11)
                                             agACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGACGCAGATAGCGac                                              >  1:2166574/2‑99 (MQ=11)
                                                           aCTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                >  1:4266002/1‑100 (MQ=35)
                                                                gctAGCGATTCAGATGCGGACAGCGATTCAGACGCAGACAGCGATTCAGACGCAGATAGCGATTCTGATGCGGACAGCGATTCAGACGCAGATAGCgatt                           >  1:5542670/3‑98 (MQ=2)
                                                                                                 |                                                                                           
TTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTC  >  NC_007793/621793‑621981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑