Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 2,531,617 (CAT)2→3 coding (233/360 nt) SAUSA300_RS13005 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077932,531,6101.T82.3% 75.9 / 12.3 34coding (240/360 nt)SAUSA300_RS13005hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/6);  new base T (0/28);  total (0/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0077932,531,6102.C82.3% 69.7 / 16.0 34coding (240/360 nt)SAUSA300_RS13005hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/6);  new base C (0/28);  total (0/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0077932,531,6103.A82.3% 78.6 / 11.8 34coding (240/360 nt)SAUSA300_RS13005hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/6);  new base A (0/28);  total (0/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCCGCATCATGTTGTTGATAATATCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACT‑‑‑TCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCATAGACATAAATATTAGCATCTTTGCGTTCAAAATG  >  NC_007793/2531516‑2531696
                                                                                               |||                                                                                      
aaTCCGCATCATGTTGTTGATAATATCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACT‑‑‑tcatc                                                                                   <  1:5569379/100‑1 (MQ=255)
      catcatGTTGTTGATAATATCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCTCTAAAATCATATGCATAATAATGCACT‑‑‑Tcatcatcatt                                                                             <  1:3481131/100‑5 (MQ=255)
       atcatGTTGTTGATAATATCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACT‑‑‑Tcatcatcattg                                                                            <  1:1952587/100‑6 (MQ=255)
        tcatGTTGTTGATAATATCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACT‑‑‑Tcatcatcatgta                                                                           <  1:1874241/100‑7 (MQ=255)
        tcatGTTGTTGATAATATCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACT‑‑‑Tcatcatcatggc                                                                           <  1:5991960/100‑7 (MQ=255)
          atGTTGTTGATAATATCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACT‑‑‑Tcatcatcattactc                                                                         <  1:815954/100‑9 (MQ=255)
            gttgttGATAATATCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACT‑‑‑Tcatcatcattgctcaa                                                                       <  1:784906/100‑11 (MQ=255)
                 tgATAATATCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGtt                                                                     <  1:4469272/100‑1 (MQ=255)
                   ataataTCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTta                                                                   <  1:5370887/100‑1 (MQ=255)
                    taataTCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGTACTTCATCATCATTACTCAAAGGTTtat                                                                  <  1:1924992/100‑1 (MQ=255)
                    taataTCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGttttt                                                                  <  1:5549404/100‑3 (MQ=255)
                    taataTCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGttggc                                                                  <  1:960857/100‑4 (MQ=255)
                    cgatcttgTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTtat                                                                  <  1:2929265/93‑1 (MQ=255)
                     cttccgatctcGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTtata                                                                 <  1:6619408/93‑1 (MQ=255)
                     aataTCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTtata                                                                 <  1:1369833/100‑1 (MQ=255)
                     aataTCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTtata                                                                 <  1:415282/100‑1 (MQ=255)
                     aataTCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTtata                                                                 <  1:3310797/100‑1 (MQ=255)
                     aataTCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTtata                                                                 <  1:6580648/100‑1 (MQ=255)
                     aataTCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTtata                                                                 <  1:4726058/100‑1 (MQ=255)
                     aataTCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTtata                                                                 <  1:2542211/100‑1 (MQ=255)
                                                  tatGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCAt                                    <  1:3375891/100‑1 (MQ=255)
                                                  tatGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCAt                                    <  1:1139594/100‑1 (MQ=255)
                                                  tatGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCAt                                    <  1:6478971/100‑1 (MQ=255)
                                                  tatGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCAt                                    <  1:3486277/100‑1 (MQ=255)
                                                  tatGATACACGTTTATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCAt                                    <  1:3429747/100‑1 (MQ=255)
                                                               tATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCATAGACATAAATAtt                       <  1:5058223/100‑1 (MQ=255)
                                                               tATCACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCATAGACATAAATAtt                       <  1:488778/100‑1 (MQ=255)
                                                                  cACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCATAGACATAAATATTAGc                    <  1:5206483/100‑1 (MQ=255)
                                                                  cACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCATAGACATAAATATTAGc                    <  1:2406879/100‑1 (MQ=255)
                                                                  cACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCATAGACATAAATATTAGc                    <  1:5695986/100‑1 (MQ=255)
                                                                  cACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCATAGACATAAATATTAGc                    <  1:1896710/100‑1 (MQ=255)
                                                                  cACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCATAGACATAAATATTAGc                    <  1:4567715/100‑1 (MQ=255)
                                                                  cACTAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTACCCTTATCATAGACATAAATAGTAGc                    <  1:4803077/100‑1 (MQ=255)
                                                                     tAAAATCATATGCATAATAATGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCATAGACATAAATATTAGCagg                 <  1:3522407/100‑3 (MQ=255)
                                                                                    aataatGCACTTCATCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCATAGACATAAATATTAGCATCTTTGCGTTCAAAATg  <  1:1790189/100‑1 (MQ=255)
                                                                                               |||                                                                                      
AATCCGCATCATGTTGTTGATAATATCGTCTCGAATCAAAATCTTGCTTATATGATACACGTTTATCACTAAAATCATATGCATAATAATGCACT‑‑‑TCATCATTACTCAAAGGTTTATAAGCTAAAATGATAATTTTATCCTTATCATAGACATAAATATTAGCATCTTTGCGTTCAAAATG  >  NC_007793/2531516‑2531696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑