| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 1,693,048 | (T)7→8 | coding (1124/1125 nt) | SAUSA300_RS08410 ← | coproporphyrinogen III oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 1,693,041 | 1 | . | T | 97.3% | 102.4 / ‑3.5 | 37 | intergenic (‑94/+6) | SAUSA300_RS08405/SAUSA300_RS08410 | HrcA family transcriptional regulator/coproporphyrinogen III oxidase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (1/35); total (1/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 | |||||||||||
CACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAA‑‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCAATCACATCGTTCTTTT > NC_007793/1692964‑1693129 | cactcactTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAgg < 1:5255856/100‑1 (MQ=255) ctcAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCaag < 1:2947108/100‑2 (MQ=255) cAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAATTTTTTTTT‑TTTAATCATTTATTAGGAAAGCTTCa > 1:6595374/1‑100 (MQ=255) tAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAGGCTTCAAAAACCTCAtt < 1:5213420/100‑1 (MQ=255) tataATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAACCCTCATTACCt < 1:3077356/100‑1 (MQ=255) taATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTAt < 1:1253410/100‑1 (MQ=255) aaTTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTagg < 1:1958975/100‑3 (MQ=255) aaTTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAA‑‑‑‑‑TTTTATTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATg < 1:6386888/100‑1 (MQ=255) aagtaagtCAATGTTAAAGCCTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCACAACCCTCATTACCTATGACTTTCCCTCTATTTGTa < 1:5046961/100‑1 (MQ=255) aagtaagtCAATGTTAAAGACTCGAAATTTCAATT‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTacggca < 1:3116038/100‑6 (MQ=255) aagtaagtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTa < 1:292885/100‑1 (MQ=255) aagtaagtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTa < 1:3217249/100‑1 (MQ=255) aagtaagtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTa < 1:3319835/100‑1 (MQ=255) aagtaagtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTa < 1:589915/100‑1 (MQ=255) aagtaagtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTa < 1:1884689/100‑1 (MQ=255) gtaagtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAg < 1:2072573/100‑1 (MQ=255) agtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGCCTTTCCCTCTATTTGTAAGTGc < 1:3861186/100‑1 (MQ=255) agtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGc < 1:3382707/100‑1 (MQ=255) agtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGc < 1:6290297/100‑1 (MQ=255) agtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGc < 1:1741956/100‑1 (MQ=255) agtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCATTAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGc < 1:6164266/100‑1 (MQ=255) gtCAATGTTAAAGACTCGAAATTTCAATT‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGc < 1:4147288/100‑1 (MQ=255) gtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCa < 1:1260743/100‑1 (MQ=255) gtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCa < 1:4487099/100‑1 (MQ=255) gtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCa < 1:4373000/100‑1 (MQ=255) gtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCa < 1:2289182/100‑1 (MQ=255) gtCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCa < 1:1715595/100‑1 (MQ=255) tCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTCCCTATGACTTTCCCTCTATTTGTAAGTGCaa < 1:5792425/100‑1 (MQ=255) tCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGCCTTTCCCTCTATTTGTAAGTGCaa < 1:5598303/100‑1 (MQ=255) tCAATGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCag < 1:4041198/100‑2 (MQ=255) cttccgatctgACTCGAAATTTCAATTTT‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGc < 1:6431343/90‑1 (MQ=255) cAATGGTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCGATTTGTAAGTGCagg < 1:4288336/100‑3 (MQ=255) aaTGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCaagc < 1:6216310/100‑3 (MQ=255) aaTGTTAAAGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCAATc < 1:5224212/100‑1 (MQ=255) aaGACTCGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCAATCACATCGt < 1:5863123/100‑1 (MQ=255) tagAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCAATCACATCGTTCttt < 1:4047827/98‑1 (MQ=255) cGAAATTTCAAT‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCAATCACATCggaagagc < 1:779802/100‑8 (MQ=255) | CACTCACTTATCTCAAGTGCTAATTATAATTTATCAAATTGGTCAAAGTAAGTCAATGTTAAAGACTCGAAATTTCAA‑‑‑‑‑TTTT‑TTTAATCATTTATTAGGAAAGCTTCAAAAACCTCATTACCTATGACTTTCCCTCTATTTGTAAGTGCAATCACATCGTTCTTTT > NC_007793/1692964‑1693129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |