Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,370,775	(T)5→6	coding (212/297 nt)	SAUSA300_RS06775 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,370,770	1	.	T	99.0%	273.3 / ‑4.1	95	coding (217/297 nt)	SAUSA300_RS06775	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (31/63);  total (31/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

AAGTTCGTTTTATTTTAGTTATTTTGTGGAATAAGAAATTTCATCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATC‑TTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAGGACTAA  >  NC_007793/1370672‑1370862                                                                                                    |                                                                                              aaGTTCGTTTTATTTTAGTTATTTTGTGGAATAAGAAATTTCATCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAGAGTACCACAAATC‑t                                                                                              >  1:4957526/1‑100 (MQ=255)              tttaGTTATTTTGTGGAATAAGAAATTTCATCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTAca                                                                                 <  1:809361/100‑2 (MQ=255)                    tATTTTGTGGAATAAGAAATTTCATCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGacaaca                                                                           <  1:5155282/100‑1 (MQ=255)                      ttttGTGGAATAAGAAATTTCATCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACAtc                                                                         <  1:1918328/100‑2 (MQ=255)                       tttGTGGAATAAGAAATTTCATCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACAttt                                                                        <  1:5061741/100‑1 (MQ=255)                       tttGTGGAATAAGAAATTTCATCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACAttt                                                                        <  1:5526402/100‑1 (MQ=255)                         ggtgGAATAAGAAATTTCATCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTaa                                                                      >  1:4434101/2‑100 (MQ=255)                                 tgctcttccgaTCTTTGTGGTCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCat                                                              <  1:204168/90‑1 (MQ=255)                                 tgctcttccgaTCTTCGTGTTCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCat                                                              <  1:2201603/90‑1 (MQ=255)                                 tgctcttccgaTCTTCGTGCTCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCat                                                              <  1:2715157/90‑1 (MQ=255)                                 aaGAAATTTCATCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCat                                                              <  1:5274518/100‑1 (MQ=255)                                 aaGAAATTTCATCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCat                                                              >  1:2305234/1‑100 (MQ=255)                                  gatcttccgaTCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAAGCTTTTTT‑CCGCTACGACAACATTTAAATCGTCatg                                                             <  1:2940124/91‑2 (MQ=255)                                      ttccgaTCTTCGTGGTCAACTTCATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATAttgg                                                         <  1:4459975/95‑2 (MQ=255)                                       tttCATCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATAttgtt                                                        <  1:706145/100‑1 (MQ=255)                                               tCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAg                                                >  1:3944184/1‑100 (MQ=255)                                               tCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAg                                                >  1:6570934/1‑100 (MQ=255)                                               tCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAg                                                >  1:1357280/1‑100 (MQ=255)                                                  gatctcaCTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCa                                             <  1:3006793/94‑1 (MQ=255)                                                   gctCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCaa                                            <  1:4916029/98‑1 (MQ=255)                                                    tctaaCTGCATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAat                                           <  1:1292554/97‑1 (MQ=255)                                                      ctaCTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAatat                                         >  1:6189599/3‑100 (MQ=255)                                                      cAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAatat                                         >  1:221631/1‑100 (MQ=255)                                                        aCTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCa                                       >  1:198737/1‑100 (MQ=255)                                                        aCTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCa                                       >  1:6497209/1‑100 (MQ=255)                                                        aCTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCa                                       >  1:307939/1‑100 (MQ=255)                                                        aCTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCa                                       <  1:5058113/100‑1 (MQ=255)                                                         cTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCag                                      <  1:2941991/100‑2 (MQ=255)                                                         cTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCaa                                      <  1:5015854/100‑1 (MQ=255)                                                          tACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAAc                                     <  1:653109/100‑1 (MQ=255)                                                           aCATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAGTATCAACa                                    >  1:5416899/1‑100 (MQ=255)                                                            cgtttACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACAt                                   <  1:2568889/98‑1 (MQ=255)                                                              tttACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCTAATATCAACATCt                                 <  1:3273932/100‑1 (MQ=255)                                                              tttACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCt                                 <  1:3181501/100‑1 (MQ=255)                                                              tttACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCt                                 <  1:383777/100‑1 (MQ=255)                                                               ttACAATAATGTGGTCATCTTCAAAGTACCCCAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCtg                                <  1:836877/100‑2 (MQ=255)                                                               ttACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCtt                                <  1:5103683/100‑1 (MQ=255)                                                                tACAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCttg                               <  1:455550/100‑2 (MQ=255)                                                                 aCAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTcc                              >  1:4321538/1‑100 (MQ=255)                                                                 aCAATAATGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTcc                              >  1:4289496/1‑100 (MQ=255)                                                                   aataatGTGGTCATCTTCAAAGTACCACAAGTCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCtt                            <  1:1849499/100‑1 (MQ=255)                                                                   aataatGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCtt                            <  1:1779235/100‑1 (MQ=255)                                                                   aataatGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCtt                            <  1:973405/100‑1 (MQ=255)                                                                   aataatGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCtc                            <  1:2928970/100‑2 (MQ=255)                                                                    ataatGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCGTATTGTTGTTCATAGCCAATATCAACATCTTCCttt                           <  1:4666382/100‑1 (MQ=255)                                                                    ataatGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCttt                           <  1:2428857/100‑1 (MQ=255)                                                                    ataatGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCttg                           <  1:1116808/100‑2 (MQ=255)                                                                    ataatGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCtgc                           <  1:1104349/100‑3 (MQ=255)                                                                    ataatGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATAGCAACATCTTCCttg                           <  1:5908082/100‑2 (MQ=255)                                                                    ataatGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAAACGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCttt                           <  1:1326087/100‑1 (MQ=255)                                                                     taatGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTccatgg                          <  1:3642151/100‑5 (MQ=255)                                                                     taatGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCttag                          <  1:485076/100‑3 (MQ=255)                                                                      aatGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCttgag                         <  1:439627/100‑4 (MQ=255)                                                                       atGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTgag                        <  1:5135966/100‑3 (MQ=255)                                                                       atGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGt                        <  1:3345325/100‑1 (MQ=255)                                                                       atGTGGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGt                        <  1:847877/100‑1 (MQ=255)                                                                       atGTGGTCATCTACAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCAAATTGTAGTGCATAGCCAATATCAACATCTTCCTTTGGt                        <  1:1372004/100‑1 (MQ=255)                                                                        tgtgGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGtt                       <  1:2778535/100‑1 (MQ=255)                                                                         gtgGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCGACATCTTCCTTTGGTTc                      <  1:1602368/100‑1 (MQ=255)                                                                         gtgGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTggggc                      <  1:3567340/100‑4 (MQ=255)                                                                         gtgGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTc                      <  1:4449369/100‑1 (MQ=255)                                                                         gtgGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTc                      <  1:1134031/100‑1 (MQ=255)                                                                         cttccgaTCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTc                      <  1:4268159/94‑1 (MQ=255)                                                                          tgGTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCa                     <  1:2661752/100‑1 (MQ=255)                                                                           ggTCATCTTCAAAGTACCGCAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAACATCAACATCTTCCTTTgggcaca                    <  1:3500081/100‑6 (MQ=255)                                                                           ggTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCTTTTggcacac                    <  1:6441484/100‑6 (MQ=255)                                                                           ggTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTggggcta                    <  1:238076/100‑6 (MQ=255)                                                                           ggTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTggggcgt                    <  1:445774/100‑6 (MQ=255)                                                                            gTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCata                   <  1:4158473/100‑3 (MQ=255)                                                                            gTCATCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCagg                   <  1:1455249/100‑3 (MQ=255)                                                                               aTCTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGt                <  1:5728520/100‑1 (MQ=255)                                                                               aTCTTCAAAGTACCACAAATC‑TTTTTGCCGCTACGACAGCATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGt                <  1:1235530/100‑1 (MQ=255)                                                                                 cTTCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTaa              <  1:3454842/100‑1 (MQ=255)                                                                                   tCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAGCATCTTCCTTTGGTTCAACTGTaaaa            >  1:620744/1‑100 (MQ=255)                                                                                   tCAAAGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTaaaa            >  1:3638419/1‑100 (MQ=255)                                                                                     aaaGTACCACAAATCTTTTTT‑CCGCTACGACAACGTTTAAATCGGCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGc          <  1:4167979/100‑1 (MQ=255)                                                                                     aaaGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGc          <  1:383657/100‑1 (MQ=255)                                                                                     aaaGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGc          <  1:846014/100‑1 (MQ=255)                                                                                     aaaGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGc          >  1:464557/1‑100 (MQ=255)                                                                                       aGTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCTACTGTAAAAGCAg        >  1:6078235/1‑100 (MQ=255)                                                                                        gTACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAgg       >  1:488334/1‑100 (MQ=255)                                                                                         tACCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGTTTCAACTGTAAAAGCAGGa      >  1:4753593/1‑100 (MQ=255)                                                                                          aCCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGTTTCAACTGTAAAAGCAGGAc     >  1:5178175/1‑100 (MQ=255)                                                                                          aCCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAGGAc     >  1:4944754/1‑100 (MQ=255)                                                                                          aCCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAGGAc     >  1:1567750/1‑100 (MQ=255)                                                                                          aCCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAGGAc     >  1:2901312/1‑100 (MQ=255)                                                                                          aCCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAGGAc     >  1:179116/1‑100 (MQ=255)                                                                                          aCCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAGGAc     >  1:355924/1‑100 (MQ=255)                                                                                          aCCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAGGAc     >  1:2308747/1‑100 (MQ=255)                                                                                          aCCACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGAAAAAGCAGGAc     >  1:761758/1‑100 (MQ=255)                                                                                           ccACAAATCTTTTTT‑CCGCTACGACAGCATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAgggtt    <  1:3037620/100‑4 (MQ=255)                                                                                           ccACAAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAGGACt    >  1:4356169/1‑100 (MQ=255)                                                                                            cacaAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAGGACTa   >  1:4620309/1‑100 (MQ=255)                                                                                             acaAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAggggtct  <  1:5259945/100‑6 (MQ=255)                                                                                             acaAATCTTTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAGGACTaa  >  1:3298942/1‑100 (MQ=255)                                                                                             acaAATCTTTTTT‑CCGCTACAACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAGGACTaa  >  1:4413427/1‑100 (MQ=255)                                                                                                    |                                                                                              AAGTTCGTTTTATTTTAGTTATTTTGTGGAATAAGAAATTTCATCTTCGTGATCAACTACATTTACAATAATGTGGTCATCTTCAAAGTACCACAAATC‑TTTTT‑CCGCTACGACAACATTTAAATCGTCATATTGTTGTTCATAGCCAATATCAACATCTTCCTTTGGTTCAACTGTAAAAGCAGGACTAA  >  NC_007793/1370672‑1370862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑