Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	730,913	+G	coding (1953/1980 nt)	SAUSA300_RS03500 →	AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	730,913	1	.	G	100.0%	80.1 / NA	32	D651E (GAC→GAG)	SAUSA300_RS03500	AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (32/0);  total (32/0)

GAGACAATTATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTTAAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGAC‑AAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAACTAC  >  NC_007793/730815‑731005                                                                                                    |                                                                                             cagaCAATTATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTTAAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGAcg                                                                                              >  1:5581964/2‑99 (MQ=255) cagaCAATTATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTTAAAAACAATGCTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGAcg                                                                                              >  1:3574109/2‑99 (MQ=255)  agaCAATTATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTTAAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGAcga                                                                                             >  1:2060258/1‑98 (MQ=255)     cAATTATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTTAAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGAC‑gaaaa                                                                                         >  1:5536818/1‑99 (MQ=255)        gcaTGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTTAAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGACGAAAATAg                                                                                       >  1:4261017/3‑100 (MQ=255)                     tggggATAGTCAAGTTTATATAAATTTTAAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAAt                                                                          >  1:887952/4‑100 (MQ=255)                                                             ttAAACGATAAATATCTAATTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGa                                  >  1:4537548/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                 >  1:4026951/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                 >  1:6452003/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                 >  1:6098924/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                 >  1:5264027/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                 >  1:5033659/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                 >  1:4457637/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                 >  1:4185409/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                 >  1:3059394/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                 >  1:2908571/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                 >  1:266524/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                 >  1:2087345/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCt                 >  1:2593858/1‑100 (MQ=255)                                                                              aaTTGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAACGGAACCCTAGTTTACTATCt                 >  1:6486035/1‑100 (MQ=255)                                                                                 tGTAATAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTaaa              >  1:3605383/1‑100 (MQ=255)                                                                                     aTAGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTg          >  1:3477196/1‑100 (MQ=255)                                                                                      caGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGa         >  1:1232557/2‑100 (MQ=255)                                                                                       aGAAACATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGAc        >  1:3774859/1‑100 (MQ=255)                                                                                        ttaaCATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACa       >  1:260075/3‑100 (MQ=255)                                                                                        aaaaCATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACa       >  1:1144506/2‑100 (MQ=255)                                                                                        aaaaCATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACa       >  1:606442/2‑100 (MQ=255)                                                                                         aaaCATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACaa      >  1:4410213/1‑100 (MQ=255)                                                                                          cccATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAAc     >  1:1131017/3‑100 (MQ=255)                                                                                           aCATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAACt    >  1:824478/1‑100 (MQ=255)                                                                                            tcttGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAACTa   >  1:5391551/3‑100 (MQ=255)                                                                                            cATTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAACTa   >  1:2735955/1‑100 (MQ=255)                                                                                             tttGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAACTAc  >  1:2442169/2‑100 (MQ=255)                                                                                             cttGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAACTAc  >  1:1627189/2‑100 (MQ=255)                                                                                             cttGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAACTAc  >  1:3495996/2‑100 (MQ=255)                                                                                             aTTGGACGAAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAACTAc  >  1:5133309/1‑100 (MQ=255)                                                                                                    |                                                                                             GAGACAATTATGAGTAATTTTGAGGATAGTCAAGTTTATATAAATTTTAAAAACAATGTTTTAAACGATAAATATCTAATTGTAATAGAAACATTGGAC‑AAAATAGTGGCAACATTAATCATTTGATTTCTAAAGAATTAAGAGATAAATATGAATGGAACCCTAGTTTACTATCTAAAATTGACAACTAC  >  NC_007793/730815‑731005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑