Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,711,162	(A)8→7	intergenic (‑382/+129)	SAUSA300_RS13905 ← / ← SAUSA300_RS13910	O‑acetyltransferase OatA/GNAT family acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,711,155	0	A	.	98.2%	209.2 / ‑2.6	56	intergenic (‑375/+136)	SAUSA300_RS13905/SAUSA300_RS13910	O‑acetyltransferase OatA/GNAT family acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (0/55);  total (0/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

ATGCATATTACTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTGAAAAAAAAGCGAAATGCAGCCTATCCTCTAATGTAAACCAAACGATATATCTCGTCAGACTTTATATTTAAACGCTATGTGTCA  >  NC_007793/2711067‑2711238                                                                                         |                                                                                    aTGCATCTTACTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGaa                                                                         <  1:4385318/100‑1 (MQ=255)     tctttACTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGc                                                                     <  1:4399303/97‑1 (MQ=255)     atcttACTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGc                                                                     <  1:3855218/97‑1 (MQ=255)          tcTGTAATGGAATCATTAAGATACGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:5491530/99‑1 (MQ=255)          agtGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:3054972/98‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:5786298/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:4769930/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:4677486/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:462134/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:6074304/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:4811960/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:4913162/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:6086473/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:5769537/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:5574998/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:3985147/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:3913560/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:106923/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:3442328/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:6120434/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:5137917/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:2776034/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:5463506/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:259357/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:2520854/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:2330254/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:6205029/100‑1 (MQ=255)          aCTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACAGGTATTG‑AAAAAAAGCGAAATGCAGCCt                                                                <  1:5236965/100‑1 (MQ=255)           cTGTAGCAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAAAGAATAAATTACCTTGTATTG‑AAAAAAAGCGAAATGCAGCCTa                                                               <  1:6528436/100‑1 (MQ=255)           cTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTGAAAAAAAAGCGAAATGCAGCCt                                                                <  1:3227645/100‑1 (MQ=255)           cTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTa                                                               <  1:273108/100‑1 (MQ=255)           cTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTa                                                               <  1:3940917/100‑1 (MQ=255)            tGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCtga                                                              <  1:4161884/100‑3 (MQ=255)            tGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTag                                                              <  1:5460243/100‑2 (MQ=255)            tGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTAt                                                              <  1:6197376/100‑1 (MQ=255)             gttacggAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAGGCGAAATGCAGCCTAta                                                             <  1:6384615/94‑2 (MQ=255)             gTAATAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACAGGTATTG‑AAAAAAAGCGAAATGCAGCCTAtg                                                             <  1:4127787/100‑2 (MQ=255)             gTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTAtg                                                             <  1:5906843/100‑2 (MQ=255)             gTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTAta                                                             <  1:1718243/100‑2 (MQ=255)             gTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCCAta                                                             <  1:4151817/100‑2 (MQ=255)             gTAACAGAATCATTAAGATATGCTATTCGAATATACTTATTCAAAATTTATATAATGAATAAATTAACATTTATTG‑AAAAAAAGCGAAATGCAGCCTAta                                                             <  1:4183709/100‑2 (MQ=255)              tAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTagag                                                            <  1:1264049/100‑4 (MQ=255)                    aaTCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTagagcacacc                                                      <  1:4974025/100‑10 (MQ=255)                    aaTCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTATCCTCTaag                                                      <  1:2802160/100‑2 (MQ=255)                       cgttAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTATCCTCTAATGTa                                                   <  1:1626756/98‑1 (MQ=255)                        aTTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTATCCTCTAATGTaa                                                  <  1:4614108/100‑1 (MQ=255)                        aTTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTATCCTCTAATGTaa                                                  <  1:2986457/100‑1 (MQ=255)                           aaGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTATCCTCTAATGTaatac                                               <  1:2022931/100‑4 (MQ=255)                            aGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTATCCTCTAATGTAAACCa                                              <  1:4987342/100‑1 (MQ=255)                            aGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTATCCTCTAATGTAAACCa                                              <  1:4981983/100‑1 (MQ=255)                              atatGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTATCCTCTAATGTAAACCaaa                                            <  1:4244425/100‑1 (MQ=255)                              atatGCTATTCGAATATACGTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTATCCTCTAATGTAAACCaaa                                            <  1:478672/100‑1 (MQ=255)                                    tATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTATCCTCTAATGTAAACCAAACGatat                                      <  1:6555956/100‑1 (MQ=255)                                          aaTATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTATCCTCTAATGTAAACCAAACGATATATCTCg                                <  1:5407946/100‑1 (MQ=255)                                          aaTATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTG‑AAAAAAAGCGAAATGCAGCCTATCCTCTAATGTAAACCAAACGATATATCTCg                                <  1:1334663/100‑1 (MQ=255)                                                                        tAAATTAACATGTAGTG‑AAAAAGAGCGAAATGCAGCTTATCCTCTAATGTAAACTAAACGATATATCGCGTCAAACTTTATATTTAAACGCTATGTGTCa  <  1:4969817/100‑1 (MQ=255)                                                                                         |                                                                                    ATGCATATTACTGTAACAGAATCATTAAGATATGCTATTCGAATATACTTTTTCAAAATTTATATAATGAATAAATTAACATGTATTGAAAAAAAAGCGAAATGCAGCCTATCCTCTAATGTAAACCAAACGATATATCTCGTCAGACTTTATATTTAAACGCTATGTGTCA  >  NC_007793/2711067‑2711238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑