Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,519,175	Δ1 bp	coding (386/588 nt)	SAUSA300_RS07370 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,519,174	0	C	.	100.0%	262.8 / NA	67	coding (387/588 nt)	SAUSA300_RS07370	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (3/64);  total (3/64)

ATAATATGGAAATTGAAGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAACCTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTAATATCACCTTGTTCAAT  >  NC_007793/1519081‑1519263                                                                                              |                                                                                          ataataTGGAAATTGAAGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGag                                                                                    <  1:97685/100‑2 (MQ=255) ataataTGGAAATTGAAGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGAt                                                                                    <  1:4085443/100‑1 (MQ=255) ataataTGGAAATTGAAGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGAt                                                                                    <  1:3644564/100‑1 (MQ=255)    atatGGAAATTGAAGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGa                                                                                 <  1:4198466/100‑1 (MQ=255)    atatGGAAATTGAAGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGa                                                                                 <  1:3851220/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACGCCCAAAGCATCGCTACACTGAGTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:6266196/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACGCTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:727891/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTTCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:4469958/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCtt                                                                    <  1:1130768/100‑2 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:2955294/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:3471432/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:4549354/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:4390681/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:4283763/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:1686983/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:3062199/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:3037698/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:5879553/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:5896068/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:6008738/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:2695369/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:3891015/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:2576299/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:683790/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:1212395/100‑1 (MQ=255)                 aGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCAGCGCTGCACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTg                                                                    <  1:5775895/100‑1 (MQ=255)                  ggTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTgt                                                                   <  1:1350442/100‑2 (MQ=255)                  ggTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGa                                                                   <  1:4470775/100‑1 (MQ=255)                  ggTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGa                                                                   <  1:4932219/100‑1 (MQ=255)                  ggTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGa                                                                   <  1:3782454/100‑1 (MQ=255)                  ggTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACGGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGa                                                                   <  1:6510333/100‑1 (MQ=255)                   gTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGag                                                                  <  1:5092321/100‑2 (MQ=255)                   gTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGag                                                                  <  1:1092505/100‑2 (MQ=255)                   gTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGaa                                                                  <  1:4210318/100‑1 (MQ=255)                        aaTAGTCAACATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGcc                                                             <  1:867917/100‑1 (MQ=255)                           aGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCAtt                                                          <  1:3064040/100‑1 (MQ=255)                            gTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAAGCATAGAACGTGTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCagag                                                         <  1:3375534/100‑4 (MQ=255)                             tCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATtat                                                        <  1:3931624/100‑1 (MQ=255)                                  ataATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACtgt                                                   <  1:3117219/100‑1 (MQ=255)                                     aTCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTggg                                                <  1:697014/100‑1 (MQ=255)                                     aTCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTggg                                                <  1:5994323/100‑1 (MQ=255)                                     aTCATGCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTggg                                                <  1:6578957/100‑1 (MQ=255)                                      tCATTCAAAAACACCCAAATCATCGCTGCACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGa                                               <  1:6174113/100‑1 (MQ=255)                                       cATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGaa                                              <  1:2141696/100‑1 (MQ=255)                                        aTTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGaaa                                             <  1:5621840/100‑1 (MQ=255)                                                 tcttccgatcttcGCTGCACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATa                                    <  1:3502885/93‑1 (MQ=255)                                                        gatctcGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAAcc                             <  1:6062305/96‑1 (MQ=255)                                                           atcGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCaag                          <  1:3914549/100‑3 (MQ=255)                                                            acGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCAttt                         >  1:1405081/2‑100 (MQ=255)                                                             cGCTACACTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTa                        >  1:2849495/1‑100 (MQ=255)                                                                 ttccgatctCCAATTATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGt                    <  1:4874256/92‑1 (MQ=255)                                                                 acacTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGt                    >  1:5338491/1‑100 (MQ=255)                                                                 acacTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGt                    <  1:676423/100‑1 (MQ=255)                                                                  atcTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAAAAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTa                   <  1:2603554/98‑1 (MQ=255)                                                                   tcTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTaa                  <  1:1795280/99‑1 (MQ=255)                                                                   acTGATTCCAATCACAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTaa                  <  1:4953323/100‑1 (MQ=255)                                                                    cTGATTCCAATCATAGAACGTTTGAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTaag                 <  1:3909319/100‑2 (MQ=255)                                                                    cTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTAat                 <  1:1711220/100‑1 (MQ=255)                                                                    cTGATTCCAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACAGTGGGAAAACATTAATACCAAACCATTTACTGTAat                 <  1:3034417/100‑1 (MQ=255)                                                                           ctctCATAGAACGTTTAAA‑CTAGGATAGAAGTAAAATGCCTGAACAGCCAATATACTGTGGGAAAACATTAATACCAAACCATTTACTGTAATATCACCt          <  1:3610777/97‑1 (MQ=255)                                                                           cAATCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTAATATCACCt          <  1:2216060/100‑1 (MQ=255)                                                                              tCATAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTAATATCACCTTGt       <  1:3253553/100‑1 (MQ=255)                                                                                ctggtccGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTAATATCACCTTGTTc     <  1:6411357/94‑1 (MQ=255)                                                                                aTAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTAATATCACCTTGTTc     <  1:1250907/100‑1 (MQ=255)                                                                                 tAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTAATATCACCTTGttag    <  1:1337934/100‑3 (MQ=255)                                                                                 tAGAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTAATATCACCTTGTTCa    <  1:437389/100‑1 (MQ=255)                                                                                   gAACGTTTAAA‑CTAGGATAGAAGTAAATTGCCTAATCAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTAATAGCACCTTGTTCaat  <  1:2875860/100‑1 (MQ=255)                                                                                              |                                                                                          ATAATATGGAAATTGAAGGTGAAAATAGTCAATATAATCATTCAAAAACACCCAAATCATCGCTACACTGATTCCAATCATAGAACGTTTAAACCTAGGATAGAAGTAAATTGCCTGAACAGCCATTATACTGTGGGAAAACATTAATACCAAACCATTTACTGTAATATCACCTTGTTCAAT  >  NC_007793/1519081‑1519263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑