Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	129,911	(T)6→5	intergenic (‑218/+203)	SAUSA300_RS00585 ← / ← SAUSA300_RS00590	peptidoglycan‑binding protein LysM/transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	129,906	0	T	.	100.0%	13.9 / NA	5	intergenic (‑213/+208)	SAUSA300_RS00585/SAUSA300_RS00590	peptidoglycan‑binding protein LysM/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (1/4);  total (1/4)

GATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGAGATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTG  >  NC_007793/129826‑129983                                                                                 |                                                                              gATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGAGATA‑TTTTTATAATGAAAGCGGtg                                                           <  1:5528528/100‑2 (MQ=255)                              ccGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGAGATA‑TTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTa                              <  1:1788826/100‑1 (MQ=255)                                                tCGATTAATAAAAACAGATAAAATATAGAGATA‑TTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGcaga            >  1:394357/1‑97 (MQ=255)                                                 cGATTAATAAAAACAGATAAAATATAGAGATA‑TTTTTATAATGAAAGCGGTTTAATACATGTGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTg           <  1:4912412/100‑1 (MQ=255)                                                          aaaaCAGATAAAATATAGAGATA‑TTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTg  <  1:684421/100‑1 (MQ=255)                                                                                 |                                                                              GATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGAGATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTG  >  NC_007793/129826‑129983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑