Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	856,210	(T)8→7	intergenic (‑34/+158)	SAUSA300_RS04140 ← / ← SAUSA300_RS04145	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	856,203	0	T	.	100.0%	61.1 / NA	17	intergenic (‑27/+165)	SAUSA300_RS04140/SAUSA300_RS04145	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (2/15);  total (2/15)

GACCTTGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATCTTTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAACACAGATTTTTTATTTTTAATCATAGCTTTAT  >  NC_007793/856125‑856285                                                                               |                                                                                   gACCTTGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCat                                                              <  1:2921158/100‑2 (MQ=255)   ctttGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACgg                                                            <  1:4145816/98‑1 (MQ=255)   ccTTGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACgt                                                            <  1:2264426/100‑2 (MQ=255)     ttGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACgggg                                                          <  1:2365158/100‑1 (MQ=255)      tGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACggggc                                                         >  1:130480/1‑99 (MQ=255)      tGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGa                                                         >  1:5263224/1‑100 (MQ=255)                        gCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCa                                       <  1:2068304/100‑1 (MQ=255)                         ccTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCaa                                      <  1:3734457/100‑2 (MQ=255)                         ccTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCAt                                      <  1:2736032/100‑1 (MQ=255)                          ctctTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCAtt                                     <  1:1035742/100‑1 (MQ=255)                                ttGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAacac                               <  1:6132715/100‑1 (MQ=255)                                ttGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAacac                               <  1:710496/100‑1 (MQ=255)                                ccgatctAATGCTTTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAacac                               <  1:1419461/94‑1 (MQ=255)                                  gTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAACACAg                             <  1:478769/100‑1 (MQ=255)                                  gTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAACACAg                             <  1:5068159/100‑1 (MQ=255)                                                             ttACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAACACAGATTTTTTATTTTTAATCATAGCTTtat  <  1:252069/100‑1 (MQ=255)                                                             ttACAAATTTATCCCATC‑TTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAACACAGATTTTTTATTTTTAATCATAGCTTtat  <  1:2508778/100‑1 (MQ=255)                                                                               |                                                                                   GACCTTGTACATTTCCCCATGTAGCCTCTTCTTGTTTTAATGCATTATTCAATGCGGGATTTACAAATTTATCCCATCTTTTTTTTATGATTTTCCGGCACGGGGACTGATTTCTTTAACACCATTAAACACAGATTTTTTATTTTTAATCATAGCTTTAT  >  NC_007793/856125‑856285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑