Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	67,917	(A)6→5	intergenic (‑935/‑49)	SAUSA300_RS00300 ← / → SAUSA300_RS00305	hypothetical protein/transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	67,912	0	A	.	100.0%	85.4 / NA	23	intergenic (‑930/‑54)	SAUSA300_RS00300/SAUSA300_RS00305	hypothetical protein/transposase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (16/7);  total (16/7)

ATATACCTACGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTTAAAAAAGTACAAGGTATTGGAAAGGAAGTGGTACCAACAGTAGTCAT  >  NC_007793/67822‑67958                                                                                           |                                               tccgatctgcGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACa                                      <  1:5882613/91‑1 (MQ=255) tccgatctgcGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACa                                      <  1:3778522/91‑1 (MQ=255)      ccTACGGTAAAGGATTAGAAGAGCTGTCAGAAGCAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGCAATTTT‑AAAAACTACAAggca                                 >  1:6653139/1‑98 (MQ=255)       gcaCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAggcag                                >  1:2943959/3‑97 (MQ=255)       gcaCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAgacat                                >  1:5325539/3‑96 (MQ=255)        caCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AACAAGTACAAggcaga                               >  1:4897918/2‑96 (MQ=255)        caCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAggccga                               >  1:2247792/2‑96 (MQ=255)        caCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAggcaag                               >  1:5067593/2‑96 (MQ=255)        caCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCAtt                               >  1:406938/2‑100 (MQ=255)         aCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAGAGTACAAGGCAtta                              >  1:4907242/1‑99 (MQ=255)         aCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACaaaccatta                              >  1:3856979/1‑93 (MQ=255)         aCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAggcaaga                              >  1:3145007/1‑95 (MQ=255)         aCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTg                              >  1:110105/1‑100 (MQ=255)         aCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTg                              >  1:2403616/1‑100 (MQ=255)         aCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTg                              >  1:5338146/1‑100 (MQ=255)         aCGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGAATTg                              >  1:1384590/1‑100 (MQ=255)                         taggCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTGGATAGGAAGTGGTAcc              >  1:3511493/4‑100 (MQ=255)                            gCTGTCAGAAGGAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTAGATCGGAAgagcacacgtc           >  1:554949/1‑90 (MQ=255)                                   gAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTGGAAAGGAAGTGGTACCAACAGTAGTc    <  1:6396514/100‑1 (MQ=255)                                   gAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTGGAAAGGAAGTGGTACCAACAGTAGTc    <  1:1104708/100‑1 (MQ=255)                                     aGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTGGAAAGGAAGTGGTACCAACAGTAGTCAt  <  1:3688598/100‑1 (MQ=255)                                     aGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTGGAAAGGAAGTGGTACCAACAGTAGTCAt  <  1:4194097/100‑1 (MQ=255)                                     aGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTT‑AAAAAGTACAAGGCATTGGAAAGGAAGTGGTACCAACAGTAGTCAt  <  1:4646498/100‑1 (MQ=255)                                                                                           |                                               ATATACCTACGGTAAAGGATTAGAAGAGCTGTCAGAAGTAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTTAAAAAAGTACAAGGTATTGGAAAGGAAGTGGTACCAACAGTAGTCAT  >  NC_007793/67822‑67958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑