Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	327	G→A	K16K (AAG→AAA)	SAUSA300_RS14705 →	replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	327	0	G	A	100.0%	217.7 / NA	73	K16K (AAG→AAA)	SAUSA300_RS14705	replication protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (73/0);  total (73/0)

GTTTTGTAAATCAAAATTATGTCCGGTTTGTAATTGGAGACGTGCTATGAAAAATAGTTATCAAGCTC‑AAAAAGTGATTGAAAAAGTAATTAAGGAAAAGCCAAAAGCACGTTGGTTGTTTTTAACACTTTCAACAAAAAATGCGATAGATGGAGATACTTTAGAAC  >  NC_007790/234‑400                                                                                               |                                                                          cttctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAg                                                                     >  1:2386300/9‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                                                   >  1:2463278/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                                                   >  1:4015525/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                                                   >  1:1577772/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                                                   >  1:1677094/7‑100 (MQ=255)   cctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                                                   >  1:2341477/7‑100 (MQ=255)    ctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccg                                                                  >  1:4974683/6‑99 (MQ=255)    ctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccg                                                                  >  1:15919/6‑99 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccga                                                                 >  1:3398162/4‑98 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAGGTGTTTAAAGAAAAGCCgaa                                                                >  1:4095568/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:4081394/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:3548849/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:2366728/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:2817270/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:2315968/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:2096273/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:2027197/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:4046673/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:4047542/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:4796477/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:1293712/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:858140/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:3036827/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTCCTATGAAAAATAGTTACCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:2924508/4‑100 (MQ=255)            cAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaattctcg                                                          >  1:48944/1‑94 (MQ=255)                  tATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggtt                                                    >  1:2600652/1‑100 (MQ=255)                  tATGACCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggtt                                                    >  1:3211488/1‑100 (MQ=255)                  ggcatCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggtt                                                    >  1:4702161/5‑100 (MQ=255)                   aTGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggttg                                                   >  1:2334450/1‑100 (MQ=255)                   aTGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggttg                                                   >  1:870521/1‑100 (MQ=255)                    tGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAGAGAAAAGCCGAATTCTCGTTGgttgt                                                  >  1:2515070/1‑100 (MQ=255)                      gccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGttt                                                >  1:3545698/5‑100 (MQ=255)                      cccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGttt                                                >  1:4614265/5‑100 (MQ=255)                       ccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGtttc                                               >  1:2682183/4‑99 (MQ=255)                            ctGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTaca                                          >  1:2771345/2‑100 (MQ=255)                              gTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTacatt                                        >  1:3483410/1‑98 (MQ=255)                                     tagaCGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAGAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATcaata                                 >  1:944209/2‑98 (MQ=255)                                              tATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTCCTTACATTATCAATACGAAATGca                        >  1:1287741/1‑99 (MQ=255)                                               aTGAAAAATATTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:3883230/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcag                       >  1:1061964/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcag                       >  1:1913313/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcag                       >  1:4878924/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:5047219/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:1466267/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:1524097/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:621091/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:1140990/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:4876111/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:4822035/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:1592195/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:3357637/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:4764692/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:1715688/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:1848037/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:453649/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:4534704/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:4362669/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:2974771/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:2869224/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:2582523/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:3853810/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:3962398/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:239193/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:2004830/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:1990837/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:1885749/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:4198777/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:4233435/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGCGa                       >  1:4675562/1‑100 (MQ=255)                                               aTGAAAAATAGTTATCAACCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTACTTACATTATCAATACGAAATGcaa                       >  1:3674162/1‑98 (MQ=255)                                                                    c‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGCAATAGGTGGAGATACGTTAGAAc  >  1:2761872/1‑100 (MQ=255)                                                                    c‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGCAATAGGTGGAGATACGTTAGAAc  >  1:4532120/1‑100 (MQ=255)                                                                    c‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGCAATAGGTGGAGATACGTTAGAAc  >  1:3331796/1‑100 (MQ=255)                                                                                               |                                                                          GTTTTGTAAATCAAAATTATGTCCGGTTTGTAATTGGAGACGTGCTATGAAAAATAGTTATCAAGCTC‑AAAAAGTGATTGAAAAAGTAATTAAGGAAAAGCCAAAAGCACGTTGGTTGTTTTTAACACTTTCAACAAAAAATGCGATAGATGGAGATACTTTAGAAC  >  NC_007790/234‑400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑