| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007790 | 1,967 | C→G | intergenic (+200/+126) | SAUSA300_RS14710 → / ← SAUSA300_RS14715 | membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007790 | 1,967 | 0 | C | G | 88.0% | 54.3 / 2.7 | 25 | intergenic (+200/+126) | SAUSA300_RS14710/SAUSA300_RS14715 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (3/0); new base G (20/2); total (23/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.30e-01 | |||||||||||
TTTTGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTG‑A‑TGTTGAGAAGAACCCTTAACTAAACTTGCAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCATTCGACAAGTTT > NC_007790/1872‑2034 | ttttGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCt > 1:3571407/1‑100 (MQ=255) ttGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTa > 1:1424125/1‑100 (MQ=255) ttGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTa > 1:4506837/1‑100 (MQ=255) ttGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTa > 1:5018753/1‑100 (MQ=255) tGACGAGATGGAAGAAAAATATGATTATCATGAGGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTaa > 1:4822400/1‑100 (MQ=255) tGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTaa > 1:1327063/1‑100 (MQ=255) tGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTaa > 1:3358931/1‑100 (MQ=255) tGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTaa > 1:3143780/1‑100 (MQ=255) tGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTaa > 1:1157575/1‑100 (MQ=255) gccGAGATGGAAGAAAAATATGATTATCAGGAGGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAgcccccag > 1:2158383/3‑95 (MQ=255) gACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTAAc > 1:1590955/1‑100 (MQ=255) atacctGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCGTTAACTa > 1:2201106/6‑100 (MQ=255) ggAAGAAAAATATGATTATCATGAAGTTAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTAACTAAACTTg > 1:4763828/1‑100 (MQ=255) ggAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGCTGAGAAGAAGCCTTAACTAAACTTg > 1:1242376/1‑100 (MQ=255) gaagaaAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTAACTAAACtaag < 1:3953809/100‑4 (MQ=255) aagaaAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGGGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTAACTAAACTTgga > 1:3555115/1‑98 (MQ=255) aagaaAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTAACTAAACTTgga > 1:2341119/1‑98 (MQ=255) agaaAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTAACTAAACTTGGAg < 1:1465436/100‑1 (MQ=255) ctgATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTG‑TGCGTTGAGAAGAAGCCTTAACTAAACTTGGAGACGAATGTCGGCATAGCGTg > 1:2416009/3‑100 (MQ=255) caTAGACGACGCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTAACTAAACTTGGAGACGAATGTCGGCATAGCGTGAGCTATTAAGcc > 1:4129629/2‑100 (MQ=255) gacgacGCATTTATGCCGAGAAAATTTATTG‑A‑TGTTGAGAAGAACCCTTAACTAAACTTGAAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCa > 1:1814264/1‑100 (MQ=255) ccgacgCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTAACTAAACTTGGAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCa > 1:1545002/2‑100 (MQ=255) ccgacgCATTTATGCCGAGAAAATTTATTG‑TGCGTTGAGAAGAAGCCTTAACTAAACTTGGAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCa > 1:2804317/2‑100 (MQ=255) acgacgCATTTATGCCGAGAAAATTTATTG‑A‑TGTTGAGAAGAACCCTTAACTAAACTTGAAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCAt > 1:686590/1‑100 (MQ=255) acgCATTTATGCCGAGAAAATTTATTGTG‑CGTTGAGAAGAAGCCTTAACTAAACTTGGAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCATTc > 1:3051170/1‑100 (MQ=255) aTGCCGAGAAAATTTATTG‑A‑TGTTGAGAAGAACCCTTAACTAAACTTGAAGACGAATGTCGGCATAGCGTGGGCTATTAAGCCGACCATTCGACAAGttt > 1:1793070/1‑100 (MQ=255) | TTTTGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTG‑A‑TGTTGAGAAGAACCCTTAACTAAACTTGCAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCATTCGACAAGTTT > NC_007790/1872‑2034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |