Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	1,778	G→A	intergenic (+11/+315)	SAUSA300_RS14710 → / ← SAUSA300_RS14715	membrane protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	1,778	0	G	A	100.0%	186.9 / NA	66	intergenic (+11/+315)	SAUSA300_RS14710/SAUSA300_RS14715	membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (61/5);  total (61/5)

TATATATTAATTCCGTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTATGAATTTCAAAAAGAATAATTAGAATATTTGAGGGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACTTTTTGTTTTTCTATAAAGATGATGCTTTTGAC  >  NC_007790/1681‑1878                                                                                                  |                                                                                                     tatatatTAATTCTGTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAAATAGAATATTTGAgagg                                                                                                    >  1:1730331/1‑100 (MQ=255)           ctctgTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAAc                                                                                          >  1:5185094/5‑100 (MQ=255)            tctgTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACg                                                                                         >  1:328989/4‑100 (MQ=255)            cctgTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACg                                                                                         >  1:5070709/4‑100 (MQ=255)             ctgTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGa                                                                                        >  1:3756017/3‑100 (MQ=255)             ctgTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGa                                                                                        >  1:3137444/3‑100 (MQ=255)              tgTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAg                                                                                       >  1:3137452/2‑100 (MQ=255)              ggTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAg                                                                                       >  1:4041634/2‑100 (MQ=255)              agTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAg                                                                                       >  1:508296/2‑100 (MQ=255)               gTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGGATAATTAGAATATTTGAGAGGATTTTGAAACGAGt                                                                                      >  1:5036987/1‑100 (MQ=255)               gTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAGACGAGt                                                                                      >  1:2682156/1‑100 (MQ=255)               gTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGt                                                                                      >  1:3579178/1‑100 (MQ=255)               gTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGt                                                                                      >  1:4127662/1‑100 (MQ=255)               gTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGt                                                                                      >  1:4384056/1‑100 (MQ=255)               gTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGt                                                                                      >  1:4516462/1‑100 (MQ=255)               gTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGt                                                                                      >  1:3095406/1‑100 (MQ=255)               gTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGt                                                                                      >  1:805799/1‑100 (MQ=255)               gTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGt                                                                                      >  1:2274533/1‑100 (MQ=255)               gTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGt                                                                                      >  1:1210915/1‑100 (MQ=255)               gTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGt                                                                                      >  1:596426/1‑100 (MQ=255)               gTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGt                                                                                      >  1:158259/1‑100 (MQ=255)                 aTGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGGTTTTGAAACGAGttt                                                                                    >  1:2194919/1‑100 (MQ=255)                 aTGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGttt                                                                                    >  1:4521783/1‑100 (MQ=255)                  tGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTttc                                                                                   >  1:3204710/1‑100 (MQ=255)                        tttGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTg                                                                             >  1:4740712/1‑100 (MQ=255)                         ttGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGt                                                                            >  1:4912738/1‑100 (MQ=255)                         ttGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGt                                                                            >  1:3484688/1‑100 (MQ=255)                          tGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGGATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGtt                                                                           >  1:4253416/1‑100 (MQ=255)                          tGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGtt                                                                           >  1:4434353/1‑100 (MQ=255)                          tGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGtt                                                                           >  1:1717519/1‑100 (MQ=255)                          agATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGtt                                                                           >  1:5223205/2‑100 (MQ=255)                           gATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTCGttt                                                                          >  1:3607589/1‑100 (MQ=255)                            aTGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGtttt                                                                         >  1:4906733/1‑100 (MQ=255)                              gggAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTca                                                                       >  1:3000125/1‑100 (MQ=255)                              gggAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTca                                                                       >  1:1845622/1‑100 (MQ=255)                                   tAAATGCTATTTATTTGGTTGTTAAGGGTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTg                                                                  >  1:4377530/1‑100 (MQ=255)                                    aaaTGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGt                                                                 >  1:557801/1‑100 (MQ=255)                                    aaaTGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGt                                                                 >  1:785627/1‑100 (MQ=255)                                     aaTGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGtt                                                                >  1:3264326/1‑100 (MQ=255)                                      aTGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGttt                                                               >  1:2778316/1‑100 (MQ=255)                                        gCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGttttt                                                             >  1:2325525/1‑100 (MQ=255)                                           atttatttGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTa                                                          >  1:4504703/1‑100 (MQ=255)                                                gttGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCt                                                     >  1:4693224/2‑100 (MQ=255)                                                cttGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCt                                                     >  1:3258899/2‑100 (MQ=255)                                                 ttGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTa                                                    >  1:1095251/1‑100 (MQ=255)                                                 gtGGTTGTTAAGGTTTTGAATTTCAAAAAGAATCATTAGAATGTTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTa                                                    >  1:2427303/2‑100 (MQ=255)                                                  tGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTAt                                                   >  1:986033/1‑100 (MQ=255)                                                  tGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTAt                                                   >  1:484386/1‑100 (MQ=255)                                                   tcttgttAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTAtt                                                  >  1:106220/3‑100 (MQ=255)                                                      tgttAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGgt                                               >  1:2814416/1‑100 (MQ=255)                                                      tgttAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGgt                                               >  1:5183793/1‑100 (MQ=255)                                                        ttAAGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGgtgc                                             <  1:4743472/100‑2 (MQ=255)                                                          aaGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGtgttt                                           <  1:184569/100‑2 (MQ=255)                                                           aGGTTTTGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGtgttgt                                          <  1:4865311/100‑1 (MQ=255)                                                                ttGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCtt                                     <  1:3466480/99‑1 (MQ=255)                                                                ctGAATTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCtt                                     >  1:2129610/2‑100 (MQ=255)                                                                   aaTTTCAAAAAGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACt                                  <  1:2489952/100‑1 (MQ=255)                                                                         aaaaaGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACTTTTTGt                            >  1:5223896/1‑100 (MQ=255)                                                                          aaaaGAATAATTAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACTTTTTGtt                           >  1:1226420/1‑100 (MQ=255)                                                                                     tAGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACTTTTTGTTTTTCTATAAAg                >  1:4506646/1‑100 (MQ=255)                                                                                      aGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACTTTTTGTTTTTCTATAAAga               >  1:4506651/1‑100 (MQ=255)                                                                                      aGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACTTTTTGTTTTTCTATAAAga               >  1:1759466/1‑100 (MQ=255)                                                                                      aGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACTTTTTGTTTTTCTATAAAga               >  1:3011330/1‑100 (MQ=255)                                                                                      aGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACTTTTTGTTTTTCTATAAAga               >  1:4721803/1‑100 (MQ=255)                                                                                      aGAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACCTTTTGTTTTTCTATAAAga               >  1:3442407/1‑100 (MQ=255)                                                                                       gAATATTTGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACTTTTTGTTTTTCTATAAAgat              >  1:1262696/1‑100 (MQ=255)                                                                                        agccccggagaGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACTTTTTGTTTTTCTATAAAgatg             >  1:982229/8‑100 (MQ=255)                                                                                             ttGAGAGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACTTTTTGTTTTTCTATAAAGatgatgat        >  1:1910614/1‑98 (MQ=255)                                                                                                  gggATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTAC‑TTTTGTTTTTCTATAAAGATGATGATTTAGAc  >  1:4150949/1‑100 (MQ=255)                                                                                                  |                                                                                                     TATATATTAATTCCGTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTATGAATTTCAAAAAGAATAATTAGAATATTTGAGGGGATTTTGAAACGAGTTTCTTCTTGTTTTCACACTGTTTTTTTATTCCTATTGGTGTTGTTGCTTACTTTTTGTTTTTCTATAAAGATGATGCTTTTGAC  >  NC_007790/1681‑1878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑