Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	1,694	C→T	P39L (CCG→CTG)	SAUSA300_RS14710 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	1,694	0	C	T	100.0%	30.8 / NA	12	P39L (CCG→CTG)	SAUSA300_RS14710	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/0);  total (12/0)

TGAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCATTTGGAAAATTTATATATATTAATTCCGTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTATGAATTTCAAAAAGAATAATTAGAATATTTGAGGGG  >  NC_007790/1602‑1780                                                                                             |                                                                                       taaaGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCATTTTGAAAATTTATATATATTAATTCTGTATGtt                                                                                 >  1:2401180/3‑100 (MQ=255)    aGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCATTTTGAAAATTTATATATATTAATTCTGTATGTTAtt                                                                              >  1:353628/1‑100 (MQ=255)               taGCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCATTTTGAAAATTTATATATATTAATTCTGTATGTTATTTTGGTGGGAAt                                                                   >  1:269815/1‑100 (MQ=255)               taGCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCATTTTGAAAATTTATATATATTAATTCTGTATGTTATTTTGATGGGAAt                                                                   >  1:3629243/1‑100 (MQ=255)                aGCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCATTTTGAAAATTTATATATATTAATTCTGTATGTTATTTTGATGGGAATa                                                                  >  1:4562075/1‑100 (MQ=255)                 gCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCATTTTGAAAATTTATATATATTAATTCTGTATGTTATTTTGATGGGagatc                                                                 >  1:4562677/1‑96 (MQ=255)                                                                 cATTTTGAAAATTTATATATATTAATTCTGTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAAtt                 >  1:1536452/1‑100 (MQ=255)                                                                 cATTTTGAAAATTTATATATATTAATTCTGTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAAtt                 >  1:1887255/1‑100 (MQ=255)                                                                 cATTTTGAAAATTTATATATATTAATTCTGTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAAtt                 >  1:3720321/1‑100 (MQ=255)                                                                 cATTTTGAAAATTTATATATATTAATTCTGTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAAtt                 >  1:3983873/1‑100 (MQ=255)                                                                       gAAAATTTATATATATTAATTCTGTATGTTATTTTGATGGGAATAAATGCTATTTATCTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAATTAGAata           >  1:5238808/1‑100 (MQ=255)                                                                                tatatatTAATTCTGTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTTTGAATTTCAAAAAGAATAAATAGAATATTTGAgagg  >  1:1730331/1‑100 (MQ=255)                                                                                             |                                                                                       TGAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCATTTGGAAAATTTATATATATTAATTCCGTATGTTATTTTGATGGGAATAAATGCTATTTATTTGGTTGTTAAGGTTATGAATTTCAAAAAGAATAATTAGAATATTTGAGGGG  >  NC_007790/1602‑1780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑