Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	1,588	A→G	N4D (AAC→GAC)	SAUSA300_RS14710 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	1,588	0	A	G	87.5%	11.4 / ‑3.9	8	N4D (AAC→GAC)	SAUSA300_RS14710	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (5/2);  total (5/3)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

CCCCCTTAAAACTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTT‑AAAAAAGGAGTGTTTATTTTGAATAAAAACACAGTGTTAGATGAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGA  >  NC_007790/1499‑1650                                                                                           |                                                               cccccTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTT‑AAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGtt                                                      <  1:1361915/100‑1 (MQ=255) cccccTTAAAACTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTT‑AAAAAAGGAGTGTTTATTTTGAATAAAAACACAGTGtt                                                      <  1:2603264/100‑1 (MQ=255)  ccccTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGtt                                                      <  1:2321733/100‑1 (MQ=255)                      aGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTT‑AAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGc                                 >  1:337650/1‑100 (MQ=255)                                       agaaGTCTTGTGATAAAGTTTTTT‑AAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGttt                >  1:591011/3‑100 (MQ=255)                                               tgtgATAAAGTTTTTT‑AAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTa        >  1:1928985/1‑100 (MQ=255)                                               tgtgATAAAGTTTTTT‑AAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTa        >  1:3991439/1‑100 (MQ=255)                                                     aaaGTTTTTT‑AAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGa  >  1:2982867/1‑100 (MQ=255)                                                                                           |                                                               CCCCCTTAAAACTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTT‑AAAAAAGGAGTGTTTATTTTGAATAAAAACACAGTGTTAGATGAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGA  >  NC_007790/1499‑1650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑