Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	1,303	T→G	intergenic (+307/‑276)	SAUSA300_RS14705 → / → SAUSA300_RS14710	replication protein/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	1,303	0	T	G	100.0%	75.7 / NA	28	intergenic (+307/‑276)	SAUSA300_RS14705/SAUSA300_RS14710	replication protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/20);  total (8/20)

ATGCTCTTACGGAGTTTTTAGAGAAAAATTAAAAATTCTCGATTTTTGATAAAAAACGCCCTGCAGGAATTTAGAAAAACAT‑GT‑GGAAGTTTTAAAGGATTTTATGCTAATTTTTAATTTGCATGTAAATGGGCAGTG‑TTAAAAAATTAGTCACTGGTTTTGC  >  NC_007790/1220‑1382                                                                                     |                                                                                  acgtagttACGGAGTTCTTAGAGAAAAAATAAAAATTCTCGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGAt                                                                  >  1:4750779/7‑100 (MQ=255)     agttACGGAGTTCTTAGAGAAAAAATAAAAATTCTCGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTa                                                              >  1:965894/3‑100 (MQ=255)     agttACGGAGTTCTTAGAGAAAAAATAAAAATTCTCGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTa                                                              >  1:4687770/3‑100 (MQ=255)            ccgatcttAGAGAAAAAATAAAAATTCTCGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTaac                                                       <  1:3972355/94‑2 (MQ=255)                       gaAAAAATAAAAATTCTCGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGc                                            <  1:1125429/100‑1 (MQ=255)                                   attCTCGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGc                                <  1:4939075/100‑1 (MQ=255)                                   attCTCGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGc                                <  1:2614021/100‑1 (MQ=255)                                   attCTCGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGc                                <  1:2614745/100‑1 (MQ=255)                                   attCTCGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGc                                <  1:392950/100‑1 (MQ=255)                                   attCTCGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAACGGGc                                <  1:4669875/100‑1 (MQ=255)                                   attCTCGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACAT‑GGGGGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGc                                <  1:1894827/100‑1 (MQ=255)                                    ttCTCGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGCa                               <  1:3918417/100‑1 (MQ=255)                                     tctcGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGCAg                              <  1:1439479/100‑1 (MQ=255)                                      ctcGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGCAgt                             <  1:1881587/100‑1 (MQ=255)                                      ctcGA‑TTTTGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACCTTTAATTTGCATGTAAATGGGCAgt                             <  1:2206515/100‑1 (MQ=255)                                        tcgattttGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGCAgtg                            <  1:2228367/96‑1 (MQ=255)                                         gATTTTTGATAAAAAACGCTCTGCAGGAATTTAGAAAAACAC‑GG‑GAAAGTTTTAAAGGATTTTATGCTAATTTTTAATTTGCATGTAAATGGACAGTG‑tt                         <  1:1018041/100‑1 (MQ=255)                                         cgattttGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGCAgtgg                           <  1:892268/97‑2 (MQ=255)                                         cgattttGATAAAAAATGTCTTGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGCAgatc                           >  1:2124412/4‑97 (MQ=255)                                                       tccgatcTGCAGGGATTTAGAAAAACAC‑GG‑GAAAGTTTTAAAGGATTTTATGCTAATTTTTAATTTGCATGTAAATGGACAGTG‑TTACAAAA‑TAGTCaag          <  1:4129487/94‑3 (MQ=255)                                                            tcTGCAGGAATTTAGAAAAACAC‑GG‑GAAAGTTTTAAAGGATTTTATGCTAATTTTTAATTTGCATGTAAATGGACAGTG‑TTACAAAA‑TAGTCATTGtttt     <  1:4090303/99‑1 (MQ=255)                                                            cttGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGCAGTGTTTAAAAAAATAGTCATTgg        <  1:4479990/98‑1 (MQ=255)                                                             ttGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGCAGTGTTTAAAAAAATAGTCATTgag       <  1:629625/99‑3 (MQ=255)                                                              tGCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGCAGTGTTTAAAAAAATAGTCATTGGtt      >  1:4525441/1‑100 (MQ=255)                                                               gCAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGCAGTGTTTAAAAAAATAGTCATTGGttt     <  1:1487178/100‑1 (MQ=255)                                                               gCAGGAATTTAGAAAAACAC‑GG‑GAAAGTTTTAAAGGATTTTATGCTAATTTTTAATTTGCATGTAAATGGACAGTG‑TTACAAAA‑TAGTCATTGTTTTTGc  >  1:4919110/1‑100 (MQ=255)                                                               gCAGGAATTTAGAAAAACAC‑GG‑GAAAGTTTTAAAGGATTTTATGCTAATTTTTAATTTGCATGTAAATGGACAGTG‑TTACAAAA‑TAGTCATTGTTTTTGc  >  1:25383/1‑100 (MQ=255)                                                                cAGGAATTTAGAAAAACATGGG‑GGAAGTTTTAAAGGATTTTATGCTAACTTTTAATTTGCATGTAAATGGGCAGTGTTTAAAAAAATAGTCATTGGtttt    >  1:18624/1‑100 (MQ=255)                                                                                     |                                                                                  ATGCTCTTACGGAGTTTTTAGAGAAAAATTAAAAATTCTCGATTTTTGATAAAAAACGCCCTGCAGGAATTTAGAAAAACAT‑GT‑GGAAGTTTTAAAGGATTTTATGCTAATTTTTAATTTGCATGTAAATGGGCAGTG‑TTAAAAAATTAGTCACTGGTTTTGC  >  NC_007790/1220‑1382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑