Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	277	G→T	intergenic (–/‑3)	– / → SAUSA300_RS14705	–/replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	277	0	G	T	97.3%	105.1 / ‑4.8	37	intergenic (–/‑3)	–/SAUSA300_RS14705	–/replication protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (36/0);  minor base C (1/0);  total (37/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

GTTTTGTAAATCAAAATTATGTCCGGTTTGTAATTGGAGACGTGCTATGAAAAATAGTTATCAAGCTC‑AAAAAGTGATTGAAAAAGTAATTAAGGAAAAGCCAAAAGCACGTTGGTTGTTTTTAACACTTTCAACA  >  NC_007790/234‑369                                            |                                                                                              cttctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAg                                      >  1:2386300/9‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                    >  1:1577772/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                    >  1:1677094/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                    >  1:2463278/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                    >  1:4015525/7‑100 (MQ=255)   cctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                    >  1:2341477/7‑100 (MQ=255)    ctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccg                                   >  1:15919/6‑99 (MQ=255)    ctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccg                                   >  1:4974683/6‑99 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccga                                  >  1:3398162/4‑98 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAGGTGTTTAAAGAAAAGCCgaa                                 >  1:4095568/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:1293712/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:3036827/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:3548849/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:4046673/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:4047542/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:4081394/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:4796477/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:858140/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:2817270/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:2027197/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:2096273/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:2315968/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:2366728/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTCCTATGAAAAATAGTTACCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                 >  1:2924508/4‑100 (MQ=255)            cAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaattctcg                           >  1:48944/1‑94 (MQ=255)                  tATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggtt                     >  1:2600652/1‑100 (MQ=255)                  tATGACCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggtt                     >  1:3211488/1‑100 (MQ=255)                  ggcatCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggtt                     >  1:4702161/5‑100 (MQ=255)                   aTGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggttg                    >  1:2334450/1‑100 (MQ=255)                   aTGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggttg                    >  1:870521/1‑100 (MQ=255)                    tGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAGAGAAAAGCCGAATTCTCGTTGgttgt                   >  1:2515070/1‑100 (MQ=255)                      gccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGttt                 >  1:3545698/5‑100 (MQ=255)                      cccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGttt                 >  1:4614265/5‑100 (MQ=255)                       ccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGtttc                >  1:2682183/4‑99 (MQ=255)                            ctGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTaca           >  1:2771345/2‑100 (MQ=255)                              gTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTacatt         >  1:3483410/1‑98 (MQ=255)                                     tagaCGTTCTATGAAAAATAGTTATCAATCCC‑AAAAAGTGATTGAAGAAGTGTTTAAAGAGAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATcaata  >  1:944209/2‑98 (MQ=255)                                            |                                                                                              GTTTTGTAAATCAAAATTATGTCCGGTTTGTAATTGGAGACGTGCTATGAAAAATAGTTATCAAGCTC‑AAAAAGTGATTGAAAAAGTAATTAAGGAAAAGCCAAAAGCACGTTGGTTGTTTTTAACACTTTCAACA  >  NC_007790/234‑369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑