D22 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	875	T→C	V199A (GTC→GCC)	SAUSA300_RS14705 →	replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	875	0	T	C	88.5%	1066.6 / 97.0	540	V199A (GTC→GCC)	SAUSA300_RS14705	replication protein
Reads supporting (aligned to +/- strand): ref base T (58/4); new base C (457/21); total (515/25)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-10

Only 100 of 544 total aligned reads displayed.

ATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGAAGATGGTAATTTGATTAATGCAAGTGATGAAGATAAAAC‑AACAGACGAAGAAGAAAAAGCACATTCAATTACCGCAATTTGGAATTTCGAAAA  >  NC_007790/778‑971

 ttatcaGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGa                                                                                                <  1:4133746/95‑1 (MQ=18)

     aaGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATg                                                                                           >  1:2815898/1‑100 (MQ=25)

         cgatctagaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTTAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTa                                                                                        <  1:3292730/92‑1 (MQ=25)

          gatctagaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTaa                                                                                       >  1:4996466/8‑100 (MQ=21)

          gatctagaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTaa                                                                                       >  1:3725715/8‑100 (MQ=25)

              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                                                                   >  1:4039526/1‑99 (MQ=25)

              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                                                                   >  1:835276/1‑99 (MQ=25)

              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                                                                   >  1:1848548/1‑99 (MQ=25)

              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                                                                   >  1:3442695/1‑99 (MQ=25)

              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                                                                   >  1:537217/1‑99 (MQ=21)

              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                                                                   >  1:182598/1‑99 (MQ=21)

              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                                                                   >  1:4596302/1‑99 (MQ=25)

              aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAACGTTGAAGAtcggaagagc                                                                                   >  1:3483975/1‑91 (MQ=21)

               agaagaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:457673/6‑100 (MQ=25)

               agaaagGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:2282217/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAATGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:5038746/3‑100 (MQ=255)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGATGATGGACATTTGa                                                                                  >  1:5174688/3‑100 (MQ=21)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:4206587/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:3597354/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:3986433/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:3935545/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:3832200/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:4410375/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:3357916/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:3226799/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:4245949/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:4263321/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:4303006/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:4321926/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:104299/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:4510933/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:4630323/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:4807986/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:4902528/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:617533/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:690635/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:77924/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:796949/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:931828/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:2703986/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:2064782/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:2016446/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:2371238/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:2399412/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:2492808/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:2588590/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:179663/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:1757457/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:1150602/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:1249685/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:1672211/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:2751239/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:2780721/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:1488622/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:2865777/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:1341726/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:1104818/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:158847/3‑100 (MQ=25)

               agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATACCGAAGATGGTAATTTGa                                                                                  >  1:4186375/3‑100 (MQ=25)

               agaaaaGGATTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                  >  1:2917856/3‑100 (MQ=25)

                 taaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGAtt                                                                                >  1:4471776/2‑100 (MQ=37)

                  caaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAATTTAATTa                                                                               >  1:3898627/2‑100 (MQ=25)

                              cGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAgaagat                                                                   >  1:3104149/1‑95 (MQ=37)

                              cGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGtgatg                                                                   >  1:2555563/1‑100 (MQ=37)

                              cGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGtgatg                                                                   >  1:3275277/1‑100 (MQ=37)

                                   aCGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGAt                                                              >  1:3386326/1‑100 (MQ=38)

                                    cGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAATTTAATTAATACAAGTGACGAAGATa                                                             <  1:4084488/100‑1 (MQ=255)

                                    cGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAATTTAATTAATACAAGTGACGAAGATa                                                             >  1:5095775/1‑100 (MQ=255)

                                             ggTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACTATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAg                                                   >  1:1642547/2‑100 (MQ=255)

                                               tcttggtggATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACGGAc                                                 >  1:1283117/4‑100 (MQ=255)

                                                tATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaac‑aagcagat                                                >  1:3137634/1‑94 (MQ=255)

                                                tATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GGCAGatc                                                >  1:1700382/1‑98 (MQ=255)

                                                tATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GAGAGACg                                                >  1:2191025/1‑100 (MQ=255)

                                                 aTGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAATAC‑GACAGatcg                                               >  1:195568/1‑97 (MQ=255)

                                                 aTGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAac‑gacaagaga                                               >  1:2886136/1‑95 (MQ=255)

                                                 aTGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAGC‑GACAGatcg                                               >  1:3553086/1‑97 (MQ=255)

                                                 aTGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGAACAGatc                                                >  1:1874474/1‑98 (MQ=255)

                                                 aTGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACga                                               >  1:3755849/1‑100 (MQ=255)

                                                 aTGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACga                                               >  1:3804825/1‑100 (MQ=255)

                                                   ggtggATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGatcgga                                             >  1:4367955/1‑95 (MQ=255)

                                                   gctggATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaac‑gactagatcgg                                             >  1:4953447/3‑92 (MQ=255)

                                                   agtggATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAac‑gacacagaaga                                             >  1:364125/2‑93 (MQ=255)

                                                    gtggATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAgccaagat                                            >  1:901335/1‑93 (MQ=255)

                                                     tggATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACTGACgaagat                                           >  1:2326862/1‑99 (MQ=255)

                                                     acgATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAATTTAATTAATACAAGTGACGAAGATAAAAC‑AACAGACgaagat                                           >  1:2957162/3‑99 (MQ=255)

                                                          tacTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGaaaa                                      <  1:2225754/98‑1 (MQ=255)

                                                                    aaagCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTcg                            >  1:3035971/1‑99 (MQ=255)

                                                                      agcATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAATTTAATTAATACAAGTGACGAAGATAAAAC‑AACAGACGAAGAGGAAAAAGCACATTCAAt                          >  1:2622231/3‑100 (MQ=255)

                                                                                   aaaCCTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTCGATTACAGCaaagatc             >  1:1016498/1‑95 (MQ=255)

                                                                                     gcctAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGaa           >  1:4849241/4‑100 (MQ=255)

                                                                                         aGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTc       >  1:474460/1‑100 (MQ=255)

                                                                                           aCGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTCGGagagatcg     >  1:5224368/1‑93 (MQ=255)

                                                                                            cGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGaa    >  1:58136/1‑100 (MQ=255)

                                                                                            cGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGaa    >  1:3521097/1‑100 (MQ=255)

                                                                                            agATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTCGATTGCAGCAATCTGGaagatcgga    >  1:942728/2‑93 (MQ=255)

                                                                                             gATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGaaagatcgga   >  1:1384277/1‑92 (MQ=255)

                                                                                             caTGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGaaa   >  1:877606/2‑100 (MQ=255)

                                                                                              aTGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGaaaa  >  1:471166/1‑100 (MQ=255)

                                                                                              aTGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGaaaa  >  1:1197030/1‑100 (MQ=255)

                                                                                              aTGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAC‑GACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGaaaa  >  1:3185821/1‑100 (MQ=255)

ATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGAAGATGGTAATTTGATTAATGCAAGTGATGAAGATAAAAC‑AACAGACGAAGAAGAAAAAGCACATTCAATTACCGCAATTTGGAATTTCGAAAA  >  NC_007790/778‑971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`