D22 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	837	G→A	L186L (TTG→TTA)	SAUSA300_RS14705 →	replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	837	0	G	A	90.5%	1488.2 / 96.2	661	L186L (TTG→TTA)	SAUSA300_RS14705	replication protein
Reads supporting (aligned to +/- strand): ref base G (58/5); new base A (573/25); total (631/30)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-07

Only 100 of 664 total aligned reads displayed.

TCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGAAGATGGTAATTTGATTAATGCAAGTGATGAAGATAAAACAACAGACGAAGAAGAA  >  NC_007790/739‑933

   gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                                                                             >  1:4139673/4‑100 (MQ=25)

   gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                                                                             >  1:2539495/4‑100 (MQ=25)

   gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                                                                             >  1:696535/4‑100 (MQ=25)

   gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                                                                             >  1:1927983/4‑100 (MQ=25)

   gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                                                                             >  1:5101669/4‑100 (MQ=25)

         ttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaa                                                                                       >  1:631797/1‑100 (MQ=25)

         ttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaa                                                                                       >  1:1739411/1‑100 (MQ=25)

          tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag                                                                                      >  1:3774744/1‑99 (MQ=25)

          tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag                                                                                      >  1:2407512/1‑99 (MQ=25)

           taCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGCGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc                                                                                     >  1:1265784/2‑98 (MQ=25)

           aaCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc                                                                                     >  1:3139091/1‑98 (MQ=25)

            aCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa                                                                                    >  1:3095282/1‑100 (MQ=25)

             cTGATGACGACGAAAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa                                                                                    >  1:3976269/1‑100 (MQ=21)

             cTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt                                                                                   >  1:3564044/1‑100 (MQ=25)

             cTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt                                                                                   >  1:4346605/1‑100 (MQ=25)

              tgatgacgacgaAAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt                                                                                   >  1:3409172/1‑100 (MQ=21)

              tgatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATa                                                                                  >  1:1437891/1‑100 (MQ=25)

              tgatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATa                                                                                  >  1:1393267/1‑100 (MQ=25)

              tgatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATa                                                                                  >  1:798720/1‑100 (MQ=25)

               gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAGAGAAAAGCATaa                                                                                 >  1:3813342/1‑100 (MQ=21)

               gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                                                                                 >  1:4080381/1‑100 (MQ=21)

               gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                                                                                 >  1:966709/1‑100 (MQ=21)

               gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                                                                                 >  1:4484459/1‑100 (MQ=21)

               gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                                                                                 >  1:3235577/1‑100 (MQ=21)

               gatgacgaca‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                                                                                 >  1:1571105/1‑100 (MQ=21)

                atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa                                                                                >  1:318838/1‑100 (MQ=21)

                 tgacgacgaaaaaaaaTCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa                                                                                >  1:4890778/10‑100 (MQ=21)

                 tgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa                                                                               >  1:2581984/1‑100 (MQ=21)

                 cgactacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa                                                                               >  1:5187845/8‑100 (MQ=21)

                  gacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAAt                                                                              >  1:2106151/7‑100 (MQ=21)

                  gacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAAt                                                                              >  1:2675207/7‑100 (MQ=21)

                   ccgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTGTGGTGGATTACTTAAAGAAAAGCATAAAAtt                                                                             >  1:5163696/6‑100 (MQ=21)

                   acgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAAtt                                                                             >  1:4225952/6‑100 (MQ=21)

                              gcgtCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAg                                                                   >  1:541174/4‑100 (MQ=21)

                                  cAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGa                                                               >  1:4923731/1‑100 (MQ=21)

                                             aaGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATg                                                    >  1:4847987/1‑100 (MQ=21)

                                                     ctagaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAAttt                                             >  1:1293365/5‑100 (MQ=25)

                                                      aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                            >  1:2768711/1‑99 (MQ=21)

                                                      aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                            >  1:2626963/1‑99 (MQ=25)

                                                      aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                            >  1:2939105/1‑99 (MQ=25)

                                                      aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                            >  1:182598/1‑99 (MQ=21)

                                                      aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                            >  1:1630639/1‑99 (MQ=25)

                                                      aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                            >  1:1353266/1‑99 (MQ=25)

                                                      aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                            >  1:3442695/1‑99 (MQ=25)

                                                      aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                            >  1:835276/1‑99 (MQ=25)

                                                      aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                            >  1:3350181/1‑99 (MQ=25)

                                                      aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAACGTTGAAGAtcggaagagc                                            >  1:3483975/1‑91 (MQ=21)

                                                       agaanaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:4276841/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:924673/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:2486377/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:1866616/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:5054818/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:2371238/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:5007881/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:4410375/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:4455457/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:4195685/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:886306/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:4593456/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:4976168/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:4666213/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:1102935/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:4807986/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:2016446/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:1150602/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:3376419/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:1218445/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:3278708/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:1020133/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:3541919/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:3029192/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:3602310/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:59495/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:3729674/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:1667642/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:5227588/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:3934046/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:2844655/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:4025150/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:779728/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:2718840/3‑100 (MQ=25)

                                                       agaaaaGGTTTGTATCAAAAACATATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                           >  1:4375513/3‑100 (MQ=255)

                                                          aaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTa                                        >  1:461781/1‑100 (MQ=37)

                                                          aaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTa                                        >  1:1789778/1‑100 (MQ=37)

                                                               tttGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAATTTAATTAATACa                                   <  1:2879732/100‑1 (MQ=25)

                                                                       gAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGagatga                           <  1:3886820/100‑1 (MQ=37)

                                                                       acccccGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGtgatga                           >  1:2796479/6‑100 (MQ=255)

                                                                        agcccGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGaa                          >  1:4254638/5‑100 (MQ=255)

                                                                             gTATGTTATGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATaa                     >  1:2235586/1‑100 (MQ=255)

                                                                               aTGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATaaat                   >  1:3686735/1‑99 (MQ=255)

                                                                                    aaGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAacgaca              >  1:4308770/1‑100 (MQ=255)

                                                                                    aaGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAacgaca              >  1:32884/1‑100 (MQ=255)

                                                                                    aaGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAacaaca              >  1:1053711/1‑100 (MQ=255)

                                                                                       ctATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAATACGACAGat           >  1:4542097/2‑99 (MQ=255)

                                                                                        tATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaacgccaagat          >  1:2302820/1‑92 (MQ=255)

                                                                                        tATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGGCAGatc          >  1:1700382/1‑98 (MQ=255)

                                                                                        tATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGAGAGACg          >  1:2191025/1‑100 (MQ=255)

                                                                                         gtggtggATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAacgacaagatc         >  1:4732522/2‑95 (MQ=255)

                                                                                              ggATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGatcggaaga    >  1:150351/1‑92 (MQ=255)

                                                                                                aTTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGatcggaagagc  >  1:2902291/1‑90 (MQ=255)

TCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGAAGATGGTAATTTGATTAATGCAAGTGATGAAGATAAAACAACAGACGAAGAAGAA  >  NC_007790/739‑933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`