D22 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	781	G→T	V168L (GTA→TTA)	SAUSA300_RS14705 →	replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	781	0	G	T	86.6%	599.0 / 74.3	279	V168L (GTA→TTA)	SAUSA300_RS14705	replication protein
Reads supporting (aligned to +/- strand): ref base G (2/0); major base T (209/31); minor base A (24/13); total (235/44)
Fisher's exact test for biased strand distribution p-value = 2.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 280 total aligned reads displayed.

AGACATTGAATCGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGA  >  NC_007790/690‑878

    gattttataacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAg                                                                                       >  1:3523420/11‑100 (MQ=255)

           gtgctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAgg                                                                                >  1:3419989/6‑100 (MQ=255)

           cGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATAATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAgg                                                                                >  1:2591394/1‑100 (MQ=255)

            tgctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGt                                                                               >  1:2368030/5‑100 (MQ=255)

            aacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaaggtt                                                                               >  1:4474383/3‑96 (MQ=255)

             gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTGACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                                                                              >  1:2432412/4‑100 (MQ=255)

             gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                                                                              >  1:2319428/4‑100 (MQ=255)

             gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGA‑CTTTTAACTGATAGCGATGAAAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                                                                              >  1:2738253/4‑100 (MQ=255)

             acAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGttt                                                                             <  1:1007062/99‑1 (MQ=255)

                aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGt                                                                           >  1:218931/1‑100 (MQ=255)

                       agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaaa                                                                   >  1:1788173/1‑100 (MQ=255)

                       agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaaa                                                                   >  1:4610224/1‑100 (MQ=255)

                       agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGaaa                                                                    >  1:2027021/1‑100 (MQ=255)

                       agaCATCAAAATATTCCGTTAAATCATCTGACCTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaaa                                                                   >  1:53122/1‑100 (MQ=255)

                         acatCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACg                                                                 >  1:1561031/4‑100 (MQ=255)

                                          tAAGTCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggt                                                 <  1:2939967/100‑1 (MQ=25)

                                          tAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggt                                                 <  1:2507888/100‑1 (MQ=37)

                                          tAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggg                                                 <  1:4513717/100‑2 (MQ=25)

                                           aaaTCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggtgg                                               <  1:2139921/100‑1 (MQ=25)

                                           aaaTCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggtgg                                               <  1:1614606/100‑1 (MQ=37)

                                           aaaTCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggtgg                                               <  1:3505570/100‑1 (MQ=37)

                                           aaaTCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtgggtg                                               <  1:2247896/100‑4 (MQ=37)

                                             atcatcTGACTTTTTAACTGATAGCGATGAAAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggtat                                               <  1:190300/100‑3 (MQ=21)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTGTCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:1621437/4‑100 (MQ=25)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:5247881/4‑100 (MQ=25)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:1128029/4‑100 (MQ=25)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:3368812/4‑100 (MQ=25)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:923941/4‑100 (MQ=25)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:833859/4‑100 (MQ=25)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:2777169/4‑100 (MQ=25)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:355697/4‑100 (MQ=25)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:4139673/4‑100 (MQ=25)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:2539495/4‑100 (MQ=25)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:4030399/4‑100 (MQ=25)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:3914241/4‑100 (MQ=25)

                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                                      >  1:3603362/4‑100 (MQ=25)

                                                          ttAACTGATGACGACG‑‑AAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag                               >  1:3844350/1‑99 (MQ=21)

                                                           tgaCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag                               >  1:3794915/3‑99 (MQ=25)

                                                           tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag                               >  1:4400138/1‑99 (MQ=25)

                                                           tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag                               >  1:2407512/1‑99 (MQ=25)

                                                           tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag                               >  1:387665/1‑99 (MQ=25)

                                                            taCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGCGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc                              >  1:1265784/2‑98 (MQ=25)

                                                            gaCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc                              >  1:4324269/2‑98 (MQ=25)

                                                            aaCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc                              >  1:3139091/1‑98 (MQ=25)

                                                            aaCTGATGACGACG‑AAAAAAATCAAGAAACTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc                              >  1:4432002/1‑98 (MQ=21)

                                                             ccTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa                             >  1:1061732/2‑100 (MQ=25)

                                                             aCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa                             >  1:2668046/1‑100 (MQ=25)

                                                             aCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa                             >  1:3095282/1‑100 (MQ=25)

                                                             aCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa                             >  1:646745/1‑100 (MQ=25)

                                                             aCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa                             >  1:5062186/1‑100 (MQ=25)

                                                              cctctgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt                            >  1:4905269/5‑100 (MQ=21)

                                                              cTGATGACGACGAAAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa                             >  1:3976269/1‑100 (MQ=21)

                                                              cTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt                            >  1:4346605/1‑100 (MQ=25)

                                                              cTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt                            >  1:1435612/1‑100 (MQ=25)

                                                              cTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt                            >  1:5177763/1‑100 (MQ=25)

                                                               tgatgacgacgaAAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt                            >  1:3409172/1‑100 (MQ=21)

                                                               tgatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATa                           >  1:1437891/1‑100 (MQ=25)

                                                               tgatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATa                           >  1:1393267/1‑100 (MQ=25)

                                                               tgatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATa                           >  1:798720/1‑100 (MQ=25)

                                                               cgatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATa                           >  1:5037094/2‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAGAGCATaa                          >  1:3713563/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:973239/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:4835869/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:4425583/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:4812568/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:750360/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:4299240/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:1080164/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:4252682/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:1590438/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:1370757/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:332818/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:1205322/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:3584290/1‑100 (MQ=21)

                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:3943258/1‑100 (MQ=21)

                                                                gataacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:3356075/10‑100 (MQ=21)

                                                                catgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa                          >  1:614154/2‑100 (MQ=21)

                                                                 atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAGGCATaaa                         >  1:4870921/1‑100 (MQ=21)

                                                                 atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa                         >  1:4495360/1‑100 (MQ=21)

                                                                 atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa                         >  1:318838/1‑100 (MQ=21)

                                                                 atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa                         <  1:498526/100‑1 (MQ=25)

                                                                  tgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa                        >  1:1304543/1‑100 (MQ=21)

                                                                  tgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa                        >  1:589211/1‑100 (MQ=21)

                                                                  gcacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa                        >  1:1667374/8‑100 (MQ=21)

                                                                  gatgacgacgaaaaaaTCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa                         >  1:3038800/11‑100 (MQ=21)

                                                                   gacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAAt                       >  1:4946933/7‑100 (MQ=21)

                                                                   gacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAAt                       >  1:3234370/7‑100 (MQ=21)

                                                                   cacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAAt                       >  1:2065812/7‑100 (MQ=21)

                                                                   cacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAAt                       >  1:4672171/7‑100 (MQ=21)

                                                                    ccgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTGTGGTGGATTACTTAAAGAAAAGCATAAAAtt                      >  1:5163696/6‑100 (MQ=21)

                                                                    ccgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAAtt                      >  1:5104890/6‑100 (MQ=21)

                                                                    acgacgaaaaaaaaTCAAGAAATTTTAAGTGATCTAG‑AAAAGGGTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAAt                       >  1:4633195/8‑100 (MQ=21)

                                                                    acgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAAtt                      >  1:2689684/6‑100 (MQ=21)

                                                                    acgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAAtt                      >  1:3431997/6‑100 (MQ=21)

                                                                             aaaaaTCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACtt              <  1:1733091/100‑1 (MQ=25)

                                                                               gaaTCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAg            >  1:1476236/2‑100 (MQ=21)

                                                                                 aTCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGAc          >  1:3281807/1‑100 (MQ=21)

                                                                                  acAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTGAAGAAAAGCATAAAATTTTAAACTTAGACg         >  1:1247937/2‑100 (MQ=21)

                                                                                         aTTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGa  <  1:2875312/100‑1 (MQ=21)

                                                                                         aTTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGAAGCCGa  <  1:4230049/100‑1 (MQ=21)

AGACATTGAATCGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGA  >  NC_007790/690‑878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`