Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007790 759 T→C D160D (GAT→GAC)  SAUSA300_RS14705 → replication protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077907590TC99.3% 408.2 / ‑5.2 152D160D (GAT→GAC) SAUSA300_RS14705replication protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (124/27);  total (125/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01

Only 100 of 223 total aligned reads displayed.

AGACATTGAATCGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAA  >  NC_007790/690‑856
                                                                     |                                                                                                  
    gattttataacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAg                                                                 <  1:3770622/90‑1 (MQ=255)
         gtctgctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑‑AAAAAATCAAGAAATTATCAGTGATCTTGAAAAAg                                                           >  1:3599873/8‑100 (MQ=255)
           gtgctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAgg                                                          >  1:3419989/6‑100 (MQ=255)
             gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTGACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                                                        >  1:2432412/4‑100 (MQ=255)
             gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                                                        >  1:1097812/4‑100 (MQ=255)
             acAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGttt                                                       <  1:1007062/99‑1 (MQ=255)
               taTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTg                                                      >  1:3909731/2‑100 (MQ=255)
               aaTCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGt                                                     <  1:80129/100‑1 (MQ=255)
               aaTCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGt                                                     <  1:3111065/100‑1 (MQ=255)
                aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGt                                                     >  1:4325601/1‑100 (MQ=255)
                aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTCGt                                                     >  1:2647157/1‑100 (MQ=255)
                    acgagaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATGAAAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATc                                                  >  1:4012739/3‑100 (MQ=255)
                     agagACATCAAAATATCCCTTTAAATCACCGGACTTTTTAACTGAGGACGCCG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaa                                               <  1:2099493/100‑1 (MQ=255)
                      cagaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaa                                              >  1:128674/2‑100 (MQ=255)
                       agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAGATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaaa                                             >  1:2375573/1‑100 (MQ=255)
                       agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaaa                                             >  1:4610224/1‑100 (MQ=255)
                       agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaaa                                             >  1:1788173/1‑100 (MQ=255)
                       agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGaaa                                              >  1:2027021/1‑100 (MQ=255)
                        gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAAc                                            >  1:1258944/1‑100 (MQ=255)
                             cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATg                                       >  1:1711521/1‑100 (MQ=255)
                             cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATg                                       >  1:567754/1‑100 (MQ=255)
                                   atTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGCTTGTATCGAAAACGTATGTTAAGt                                 >  1:3360514/1‑100 (MQ=255)
                                          tAAGTCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggt                           <  1:2939967/100‑1 (MQ=25)
                                          tAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggt                           <  1:2507888/100‑1 (MQ=37)
                                          tAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggg                           <  1:4513717/100‑2 (MQ=25)
                                           aaaTCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggtgg                         <  1:3857916/100‑1 (MQ=37)
                                           aaaTCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggtgg                         <  1:3505570/100‑1 (MQ=37)
                                            aaTCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggtat                         <  1:4240592/100‑3 (MQ=25)
                                             atcatcTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGag                       <  1:909288/100‑2 (MQ=37)
                                               catcTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGAtta                     >  1:2344796/1‑99 (MQ=37)
                                                  cTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCt                   <  1:1537373/100‑1 (MQ=25)
                                                    gactttttAACTGATGACGACGAAAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTa                 >  1:5172189/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTGTCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:1621437/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:1128029/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:3914241/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:2539495/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:3157555/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:3603362/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:3386320/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:696535/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:833859/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:528497/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:513785/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:1565987/4‑100 (MQ=25)
                                                    gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                >  1:5194442/4‑100 (MQ=25)
                                                        ttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaa            >  1:3522820/1‑100 (MQ=25)
                                                          ttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaa          >  1:2805815/1‑100 (MQ=25)
                                                          ttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaa          >  1:4460799/1‑100 (MQ=25)
                                                           tgaCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag         >  1:3794915/3‑99 (MQ=25)
                                                           tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag         >  1:4123324/1‑99 (MQ=25)
                                                           tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag         >  1:3544230/1‑99 (MQ=25)
                                                           tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag         >  1:2244877/1‑99 (MQ=25)
                                                           tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag         >  1:2407512/1‑99 (MQ=25)
                                                           tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag         >  1:4400138/1‑99 (MQ=25)
                                                            gaCTGATGACGACG‑AAAAAAATCAAGAAATTTCAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc        >  1:145570/2‑98 (MQ=21)
                                                            aaCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc        >  1:2140773/1‑98 (MQ=25)
                                                            aaCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc        >  1:3949462/1‑98 (MQ=25)
                                                             ccTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa       >  1:1061732/2‑100 (MQ=25)
                                                             aCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa       >  1:4662386/1‑100 (MQ=25)
                                                             aCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa       >  1:5062186/1‑100 (MQ=25)
                                                              cctctgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt      >  1:4905269/5‑100 (MQ=21)
                                                              cTGATGACGACGAAAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa       >  1:3976269/1‑100 (MQ=21)
                                                              cTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt      >  1:3564044/1‑100 (MQ=25)
                                                              cTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt      >  1:4346605/1‑100 (MQ=25)
                                                              cTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt      >  1:1372099/1‑100 (MQ=25)
                                                               tgatgacgacgaAAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt      >  1:3409172/1‑100 (MQ=21)
                                                               tgatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATa     >  1:642544/1‑100 (MQ=25)
                                                               tgatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATa     >  1:1393267/1‑100 (MQ=25)
                                                               cgatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATa     >  1:5037094/2‑100 (MQ=21)
                                                               agatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAACGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATa     >  1:3283499/2‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:750360/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:121863/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:1435661/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:1590438/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:1074613/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:4835869/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:4812568/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:472317/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:966709/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:4299240/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:382535/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:332818/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:3235577/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:3082291/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:4258206/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:1851062/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:2756570/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:4425583/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:4484459/1‑100 (MQ=21)
                                                                gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:2259417/1‑100 (MQ=21)
                                                                catgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa    >  1:614154/2‑100 (MQ=21)
                                                                 atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa   <  1:498526/100‑1 (MQ=25)
                                                                 atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa   >  1:318838/1‑100 (MQ=21)
                                                                 atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa   >  1:4495360/1‑100 (MQ=21)
                                                                  tgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa  >  1:1610973/1‑100 (MQ=21)
                                                                  tgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa  >  1:2707320/1‑100 (MQ=21)
                                                                  tgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa  >  1:1408398/1‑100 (MQ=21)
                                                                  tgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa  >  1:2581984/1‑100 (MQ=21)
                                                                  tgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa  >  1:1304543/1‑100 (MQ=21)
                                                                  tgacgacg‑aAAAAAATCAAGAAATTTTAAGAGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGATATGGAGGATTACTTAAAGAAAAGCATaaaa  >  1:2876568/1‑100 (MQ=18)
                                                                     |                                                                                                  
AGACATTGAATCGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAA  >  NC_007790/690‑856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑