| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007790 | 1,954 | 2 bp→TGC | intergenic (+187/+138) | SAUSA300_RS14710 → / ← SAUSA300_RS14715 | membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007790 | 1,953 | 1 | . | T | 91.7% | 61.5 / ‑0.3 | 24 | intergenic (+186/+140) | SAUSA300_RS14710/SAUSA300_RS14715 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base T (17/5); total (19/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
| * | NC_007790 | 1,954 | 0 | A | G | 88.0% | 48.8 / 0.1 | 25 | intergenic (+187/+139) | SAUSA300_RS14710/SAUSA300_RS14715 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base G (17/5); total (20/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
| * | NC_007790 | 1,955 | 0 | T | C | 88.0% | 45.5 / ‑0.1 | 25 | intergenic (+188/+138) | SAUSA300_RS14710/SAUSA300_RS14715 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base C (17/5); total (20/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
GATGCTTTTGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTG‑ATGTTGAGAAGAACCCTTAACTAAACTTGCAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCATTCGACAAGTTTTGGGATTGTTAAGGGTTC > NC_007790/1867‑2052 ||| gatgatTTTGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGagaaga < 1:125353/100‑1 (MQ=255)gatgatTTTGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGagaaga > 1:1030939/1‑100 (MQ=255) tgattttGACGAGATGGAAGAAAAATATGATTATCATGAGGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAgaagaag > 1:1094791/4‑99 (MQ=255) tttGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCtt > 1:4612656/1‑100 (MQ=255) tttGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCtt > 1:3154310/1‑100 (MQ=255) cttGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCtt < 1:3636334/99‑1 (MQ=255) ttGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTa > 1:265200/1‑100 (MQ=255) tGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGagaagatcggaaga > 1:1403690/1‑92 (MQ=255) tGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTaa > 1:1658630/1‑100 (MQ=255) tGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTaa > 1:730542/1‑100 (MQ=255) tGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTaa > 1:2248526/1‑100 (MQ=255) tGACGAGATGGAAGAAAAATATGATTATCATGAAGATAAT‑AAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTAAc > 1:734945/1‑100 (MQ=255) gACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTAAc > 1:2660365/1‑100 (MQ=255) ccGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATTCCGAGAAAATTTATTGTGCGTTGAGAAGAAACCTTAACt > 1:5054260/2‑100 (MQ=255) cGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTAACTa > 1:2168409/1‑100 (MQ=255) cGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTAACTa > 1:5200938/1‑100 (MQ=255) ggAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTAACTAATCTTg > 1:5982382/1‑100 (MQ=255) gaagaaAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTAACTAAACTTgg < 1:2294083/100‑2 (MQ=255) gaagaaAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTAACTAAACTTgg < 1:3322774/100‑2 (MQ=255) gaagaaAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTAACTAAACTTgg < 1:2090425/100‑2 (MQ=255) ccgacgCATTTATGCCGAGAAAATTTATTG‑ATGTTGAGAAGAACCCTTAACTAAACTTGAAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCAt > 1:390854/2‑100 (MQ=255) gccccgtGCCGAGAAAATTTATTG‑ATGTTGAGAAGAACCCTTAACTAAACTTGAAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCATTCGACa > 1:1045771/7‑100 (MQ=255) aTGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTAACTAAACTTGGAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCATTCGACAAGtt > 1:2800528/1‑100 (MQ=255) aTGCCGAGAAAATTTATTGTGCGTTGAGAAGAAGCCTTAACTAAACTTGGAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCATTCGACAAGtt > 1:301323/1‑100 (MQ=255) g‑ATGTTGAGAAGAACCCTTAACTAAACTTGAAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCATTCGACAAGTTTTGGGATTGTTAAGGGTTc > 1:2049674/1‑100 (MQ=255) ||| GATGCTTTTGACGAGATGGAAGAAAAATATGATTATCATGAAGATAATAAAAAATAGACGACGCATTTATGCCGAGAAAATTTATTG‑ATGTTGAGAAGAACCCTTAACTAAACTTGCAGACGAATGTCGGCATAGCGTGAGCTATTAAGCCGACCATTCGACAAGTTTTGGGATTGTTAAGGGTTC > NC_007790/1867‑2052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |