Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	336	A→G	P19P (CCA→CCG)	SAUSA300_RS14705 →	replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	336	0	A	G	100.0%	115.8 / NA	45	P19P (CCA→CCG)	SAUSA300_RS14705	replication protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/1);  total (44/1)

GTAAATCAAAATTATGTCCGGTTTGTAATTGGAGACGTGCTATGAAAAATAGTTATCAAGCTCAAAAAGTGATTGAAAAAGTAATTAAGGAAAAGCCAAAAGCACGTTGGTTGTTTTTAACACTTTCAACAAAAAATGCGATAGATGGAGATACTTTAGAAC  >  NC_007790/239‑400                                                                                                  |                                                                 ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:1084863/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:932323/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:704044/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:5832145/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:5812605/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:5329854/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:5165880/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:4844862/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:4598836/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:3980831/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:37198/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:3717605/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:3616043/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:3361949/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:3208911/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:1884839/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:2633601/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGCTTAAAGAAAAGCCgaa                                                                >  1:1282400/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGAGTTTAAAGAAAAGCCgaa                                                                >  1:3745117/4‑100 (MQ=255) ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGAATGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:4018120/4‑100 (MQ=255) ggcaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGCTCTATGAAAAATAGGTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:817120/4‑100 (MQ=255) gataaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                                                >  1:2941922/4‑100 (MQ=255)              aTGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAACGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggttg                                                   >  1:4800303/1‑100 (MQ=255)               gtgccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgt                                                  >  1:2818388/7‑100 (MQ=255)                gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGCAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                                                 >  1:26516/1‑100 (MQ=255)                gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                                                 >  1:4454546/1‑100 (MQ=255)                gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                                                 >  1:4435171/1‑100 (MQ=255)                gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                                                 >  1:4209494/1‑100 (MQ=255)                gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                                                 >  1:4088399/1‑100 (MQ=255)                gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                                                 >  1:2111821/1‑100 (MQ=255)                 tCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGTATTCTCGTTGGTTGttt                                                >  1:2924566/1‑100 (MQ=255)                  ccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGtttc                                               >  1:6040936/4‑99 (MQ=255)                  ccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGtttc                                               >  1:1310397/4‑99 (MQ=255)                       ttGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTaca                                          >  1:1031837/1‑100 (MQ=255)                       ttGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTaca                                          >  1:1903985/1‑100 (MQ=255)                        tGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTacat                                         >  1:581826/1‑99 (MQ=255)                         gTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTacatt                                        >  1:4934173/1‑98 (MQ=255)                              tGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATcag                                   >  1:4141711/1‑99 (MQ=255)                              tGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATcaa                                   >  1:910245/1‑100 (MQ=255)                                        cTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGc                         >  1:273290/1‑100 (MQ=255)                                          aTGAAATATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:5580078/1‑98 (MQ=255)                                          aTGAAACATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGGATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcag                       >  1:1980457/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGCAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:3703264/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGGATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:1211021/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATggca                       >  1:2979799/1‑97 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:5621481/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:2055898/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:5674075/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:4030799/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:2800209/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:3776960/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:708058/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:90541/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:224123/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:5598425/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:1434987/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:5262388/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:1473751/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:1863920/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:4785851/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:4752449/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:4702177/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:2474026/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:4434220/1‑98 (MQ=255)                                          aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGCTGTTTCTTACATTATCAATACGAAATGcaa                       >  1:85801/1‑98 (MQ=255)                                           tGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGCAAt                      <  1:2872536/100‑1 (MQ=255)                                                               cAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGCAATAGGTGGAGATACGTTAGAAc  >  1:4319854/1‑100 (MQ=255)                                                                                                  |                                                                 GTAAATCAAAATTATGTCCGGTTTGTAATTGGAGACGTGCTATGAAAAATAGTTATCAAGCTCAAAAAGTGATTGAAAAAGTAATTAAGGAAAAGCCAAAAGCACGTTGGTTGTTTTTAACACTTTCAACAAAAAATGCGATAGATGGAGATACTTTAGAAC  >  NC_007790/239‑400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑