Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	1,588	A→G	N4D (AAC→GAC)	SAUSA300_RS14710 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	1,588	0	A	G	100.0%	51.5 / NA	20	N4D (AAC→GAC)	SAUSA300_RS14710	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/5);  total (15/5)

ATACCCCCTTAAAACTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTGTTTATTTTGAATAAAAACACAGTGTTAGATGAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCATTTGGAAAATTTATATA  >  NC_007790/1496‑1684                                                                                             |                                                                                                 aTACCCCCTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAgt                                                                                           >  1:3990512/1‑100 (MQ=255)   aCCCCCTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAgtgt                                                                                         <  1:5902710/100‑1 (MQ=255)    cccccTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGtt                                                                                        <  1:2420925/100‑1 (MQ=255)    cccccTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGtt                                                                                        <  1:3132748/100‑1 (MQ=255)    cccccTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGtt                                                                                        <  1:5039293/100‑1 (MQ=255)                   aaaTAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGt                                                                         <  1:950307/100‑1 (MQ=255)                          ggTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCa                                                                  >  1:2607006/1‑100 (MQ=255)                                          gTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATATTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGttt                                                  >  1:6066737/1‑100 (MQ=255)                                            aaGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTc                                                >  1:1228287/1‑100 (MQ=255)                                                cTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCtttt                                            >  1:595129/1‑100 (MQ=255)                                                 ctgtgATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCttttt                                           >  1:3979953/2‑100 (MQ=255)                                                  tgtgATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACGCCGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTa                                          >  1:6102128/1‑100 (MQ=255)                                                  tgtgATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTa                                          >  1:4560922/1‑100 (MQ=255)                                                  tgtgATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTa                                          >  1:588003/1‑100 (MQ=255)                                                  tgtgATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTa                                          >  1:2182066/1‑100 (MQ=255)                                                   gtgATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTAc                                         >  1:4162163/1‑100 (MQ=255)                                                    tgATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACt                                        >  1:734205/1‑100 (MQ=255)                                                     gATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACtt                                       >  1:1226096/1‑100 (MQ=255)                                                        aaaGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGa                                    >  1:3919042/1‑100 (MQ=255)                                                                              gttATTTTGAATAAAGACACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCAttttg              >  1:5791129/2‑98 (MQ=255)                                                                                          aaagaCACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCATTTTGAAAATTtatata  >  1:4101120/1‑100 (MQ=255)                                                                                          aaagaCACAGTGTTAGATAAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCATTTTGAAAATTtatata  >  1:4220468/1‑100 (MQ=255)                                                                                             |                                                                                                 ATACCCCCTTAAAACTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTGTTTATTTTGAATAAAAACACAGTGTTAGATGAAGGTTACGTTATAGCAACTATTTTAAATGTTTTCTTTTTACTTGGATTAATTTTTATATCTCATTTGGAAAATTTATATA  >  NC_007790/1496‑1684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑