Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	1,510	C→T	intergenic (+514/‑69)	SAUSA300_RS14705 → / → SAUSA300_RS14710	replication protein/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	1,510	0	C	T	100.0%	94.3 / NA	33	intergenic (+514/‑69)	SAUSA300_RS14705/SAUSA300_RS14710	replication protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/7);  total (26/7)

ATTTGGCTCATATTTGCGTTTTAAGAGCTTGTATATATTTTTAAGTTAAAAACCTAT‑ATGA‑TAC‑CCCCTAAACCTTTAAAATACCCCCTTAAAACTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTGTTTATTTTGAATAAAAACACAGTGTT  >  NC_007790/1416‑1598                                                                                                  |                                                                                         atttGGCTCATATTTTCGTTTTAAAAGCGTATATA‑AGTTTTGGATAGAAAAAATAT‑ATGATTTA‑CCCCTAAACCTTTAAAAT‑GCCCCTTAAAATTCaaaa                                                                                    >  1:3121297/1‑100 (MQ=255) atttGGCTCATATTTGCGTTTTAAGAGCTTGTATAGGTTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCaaa                                                                                     >  1:28875/1‑100 (MQ=255) atttGGCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCaaa                                                                                     >  1:4557846/1‑100 (MQ=255)  cttGGCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCaaaa                                                                                    >  1:919550/2‑100 (MQ=255)   ttGGCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAAt                                                                                   >  1:1747726/1‑100 (MQ=255)   ttGGCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAAt                                                                                   >  1:4967305/1‑100 (MQ=255)   ttGGCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAAt                                                                                   >  1:424797/1‑100 (MQ=255)   ctGGCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAAt                                                                                   >  1:3895711/2‑100 (MQ=255)    tagCTCATATTTTCGTTTTAAAAGCGTATATA‑AGTTTTGGATAGAAAAAATAT‑ATGATTTA‑CCCCTAAACCTTTAAAAT‑GCCCCTTAAAATTCAAAATaa                                                                                 >  1:2858025/3‑100 (MQ=255)    tGGCTCATATTTTCGTTTTAAAAGCGTATATA‑AGTTTTGGATAGAAAAAATAT‑ATGATTTA‑CCCCTAAACCTTTAAAAT‑GCCCCTTAAAATTCAAAATaa                                                                                 >  1:1992382/1‑100 (MQ=255)    tGGCTCATATTTTCGTTTTAAAAGCGTATATA‑AGTTTTGGATAGAAAAAATAT‑ATGATTTA‑CCCCTAAACCTTTAAAAT‑GCCCCTTAAAATTCAAAATaa                                                                                 >  1:665836/1‑100 (MQ=255)    tGGCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAATa                                                                                  >  1:1335662/1‑100 (MQ=255)    tGGCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAATa                                                                                  >  1:4244771/1‑100 (MQ=255)    tGGCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAATa                                                                                  >  1:2783531/1‑100 (MQ=255)    tGGCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGA‑TTTACCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAATa                                                                                  >  1:974312/1‑100 (MQ=255)     ggCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTATAAGGT‑TTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAATaa                                                                                 >  1:1240371/1‑100 (MQ=255)     ggCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAATaa                                                                                 >  1:4199925/1‑100 (MQ=255)     ggCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAATaa                                                                                 >  1:4299006/1‑100 (MQ=255)     ggCTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAATaa                                                                                 >  1:4658108/1‑100 (MQ=255)     cccTCATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAATaa                                                                                 >  1:4976849/3‑100 (MQ=255)         cATATTTGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCACTTAAAATTCAAAATAACGGt                                                                             >  1:4301990/1‑100 (MQ=255)          gggggtGCGTTTTAAGAGCTTGTATAGATTTTTAAG‑TAGAAAACTAT‑AAGATTTA‑CCTCTAAATCTTTAAAATACCCTCTTAAAATTCAAAATAAAGGtg                                                                            >  1:3925877/6‑99 (MQ=255)           tatTTTCGTTTTAAAAGCGTATATA‑AGTTTTGGATAGAAAAAATAT‑ATGATTTA‑CCCCTAAACCTTTAAAAT‑GCCCCTTAAAATTCAAAATAAGGGCAtg                                                                          <  1:5984109/100‑2 (MQ=255)                                           atgtaggaaaaCTAT‑ATGATTTA‑CCCCTAAATCTTTAAAATACCCCCTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGtt                                            >  1:802396/8‑100 (MQ=255)                                                                   accccTAAATCTTTAAAATACCCCCTTAAAATTCAAAATAAAGGAATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTAtt                      >  1:4710401/2‑100 (MQ=255)                                                                     cccTAAATCTTTAAAATACCCCCTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTAtttt                    >  1:3067122/1‑100 (MQ=255)                                                                      tcTAAATCTTTAAAATACCCTCTTAAAATTCAAAATAAAGGTGTTTATAATTTGAATAAGTCCTGAGATAAAGTTTTTT‑‑AAAAGGAGTGGTT‑TTTTGaag                <  1:5393523/99‑2 (MQ=255)                                                                            tcTTTAAAATACCCTCTTAAAATTCAAAATAAAGGTGTTTATAATTTGAATAAGTCCTGAGATAAAGTTTTTT‑‑AAAAGGAGTGGTT‑TTTTGaagaggttt          <  1:3011904/99‑8 (MQ=255)                                                                                    aTACCCCCTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAgt     >  1:3990512/1‑100 (MQ=255)                                                                                      aCCCCCTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAgtgt   <  1:5902710/100‑1 (MQ=255)                                                                                       cccccTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGtt  <  1:5039293/100‑1 (MQ=255)                                                                                       cccccTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGtt  <  1:2420925/100‑1 (MQ=255)                                                                                       cccccTTAAAATTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTCTTTATTTTGAATAAAGACACAGTGtt  <  1:3132748/100‑1 (MQ=255)                                                                                                  |                                                                                         ATTTGGCTCATATTTGCGTTTTAAGAGCTTGTATATATTTTTAAGTTAAAAACCTAT‑ATGA‑TAC‑CCCCTAAACCTTTAAAATACCCCCTTAAAACTCAAAATAAAGGTATTTATAATTTGAGTAAGTCTTGTGATAAAGTTTTTTAAAAAAGGAGTGTTTATTTTGAATAAAAACACAGTGTT  >  NC_007790/1416‑1598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑