Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007790 875 T→C V199A (GTC→GCC)  SAUSA300_RS14705 → replication protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077908750TC88.3% 1132.8 / 106.3 564V199A (GTC→GCC) SAUSA300_RS14705replication protein
Reads supporting (aligned to +/- strand):  ref base T (60/6);  new base C (466/32);  total (526/38)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-20

Only 100 of 566 total aligned reads displayed.

AATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGAAGATGGTAATTTGATTAATGCAAGTGATGAAGATAAAACAACAGACGAAGAAGAAAAAGCACATTCAATTACCGCAATTTGGAATTTCGAAAA  >  NC_007790/777‑971
                                                                                                  |                                                                                                
  ttatcaGTGCTCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGttgt                                                                                               <  1:3352698/95‑4 (MQ=18)
      aaGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATg                                                                                          >  1:4571298/1‑100 (MQ=21)
       cgTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCCAAGAtcg                                                                                         >  1:1542636/2‑98 (MQ=18)
       aatGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATgg                                                                                         >  1:2964497/3‑100 (MQ=21)
        gTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGt                                                                                        >  1:770488/1‑100 (MQ=21)
        gTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGt                                                                                        <  1:177920/100‑1 (MQ=21)
           gatctagaaaaGGTTTGTATCGAAAACGTATGTTAAGTTGTGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTaa                                                                                      >  1:2028773/8‑100 (MQ=21)
           gatctagaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTaa                                                                                      >  1:3217636/8‑100 (MQ=21)
              ctagaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAAttt                                                                                   >  1:5749545/5‑100 (MQ=25)
               aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGTAAttta                                                                                  >  1:824668/1‑99 (MQ=21)
               aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATGTTGAAGATGGCAAttta                                                                                  >  1:863489/1‑99 (MQ=21)
               aGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTAGATTGCTTAAACAAAAGCATAAAATTTTAAATTTAGATAATATTGAAGAtcggaagagc                                                                                  >  1:4786650/1‑91 (MQ=18)
                agaagaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:318026/6‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGTTTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:1192773/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTACAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:3906244/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAACGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:5860920/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGGAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:1412427/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:3451401/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:100123/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:3804534/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:4110091/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:3296558/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:4351406/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:4424812/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:4847821/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:4497811/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:4526522/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:465813/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:4732901/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:3769183/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:4911146/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:4994327/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:51795/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:5430619/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:5451745/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:606494/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:709692/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:901306/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:933531/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:1634814/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:236951/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:2345135/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:2321357/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:2221924/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:2486701/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:2097336/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:2004081/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:1914675/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:1709068/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:1476384/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:2722207/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:1387180/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:1231147/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:114439/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:1069404/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTCAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:252223/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGGGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:3731660/3‑100 (MQ=25)
                agaaaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGCGGATTACTTAAAGAAAAGCATAAACTTTTAAACTTAGACGATGCCGAAGATGGTAATTTGa                                                                                 >  1:1328355/3‑100 (MQ=21)
                   aaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTa                                                                              >  1:5921570/1‑100 (MQ=25)
                   aaaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTa                                                                              >  1:307452/1‑100 (MQ=25)
                    aaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTaa                                                                             >  1:3143759/1‑100 (MQ=25)
                    aaGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTaa                                                                             >  1:1863680/1‑100 (MQ=25)
                       gTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTaacta                                                                          <  1:4155722/100‑4 (MQ=25)
                       acttGTCTCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAGAGCATAAAATTTTAAACTTAGTCGATGCCGAAGATGGTAATTTGATTAAtac                                                                          >  1:2829192/3‑98 (MQ=25)
                                  caaCGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAg                                                               >  1:527100/2‑100 (MQ=37)
                                   aaCGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGa                                                              >  1:3386570/1‑100 (MQ=38)
                                   aaCGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGa                                                              >  1:2184842/1‑100 (MQ=38)
                                   aaCGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGa                                                              >  1:5327298/1‑100 (MQ=38)
                                    ccGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGAt                                                             >  1:4184402/2‑100 (MQ=255)
                                    aCGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAAGACAAgatcggaagag                                                             >  1:403285/1‑90 (MQ=25)
                                     cGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGtgatggatggt                                                            <  1:42241/100‑7 (MQ=255)
                                            tAAGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaacgtc                                                     <  1:2659226/100‑4 (MQ=255)
                                             caGTTATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGGTTAATACAAGTGATGAAGATAAAacgaca                                                    >  1:1674667/2‑100 (MQ=255)
                                                 tATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGatc                                                >  1:3941171/1‑98 (MQ=255)
                                                 tATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGAca                                                >  1:3598581/1‑99 (MQ=255)
                                                 tATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACg                                                >  1:5365005/1‑100 (MQ=255)
                                                 tATGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACAACAGaga                                                >  1:5132/1‑98 (MQ=255)
                                                  aTGGTGGATTACTTAAAGAGAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAATCGACAGatcg                                               >  1:3880655/1‑97 (MQ=255)
                                                  aTGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaacgatcagatc                                               >  1:599918/1‑91 (MQ=255)
                                                  aTGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAacaacaaagat                                               >  1:1035788/1‑95 (MQ=255)
                                                  aTGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACga                                               <  1:495852/100‑1 (MQ=255)
                                                  aTGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACAACAGatcg                                               >  1:5818753/1‑97 (MQ=255)
                                                  aTGGTGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACAACAGatcg                                               >  1:5235762/1‑97 (MQ=255)
                                                  aTGGGGGATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAaacggacagatc                                               >  1:5628513/1‑91 (MQ=255)
                                                    tgtggATTACTTAAAGAAAAGCATAAAATTTTAAACTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACAACAGatcgga                                             >  1:5074517/2‑95 (MQ=255)
                                                                             aaaTTTTAAACCTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGc                    <  1:3032440/100‑1 (MQ=255)
                                                                                    aaaCTTAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTgg             >  1:2795202/1‑100 (MQ=255)
                                                                                     aatttAGATAATGTTGAAGATGGTAATTTAATTAATACAAGTGACGAAGATAAAACAACAGACGAAGAGGAAAAAGCACATTCAATTACAGCAATCTGGa            <  1:66264/97‑1 (MQ=255)
                                                                                         tAGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATCTGGaaaga        >  1:2577506/1‑97 (MQ=255)
                                                                                          aGACGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAAGGACAGACGAAGAAGAAAAAGCACATTCGATGACAGCAAtagatcggaag       >  1:1956062/1‑90 (MQ=255)
                                                                                            ccGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGa     >  1:460131/2‑100 (MQ=255)
                                                                                            aCGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGaagatcgg     >  1:1275494/1‑94 (MQ=255)
                                                                                            aCGATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGa     >  1:2903390/1‑100 (MQ=255)
                                                                                             tgATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGaagatcgga    >  1:4307438/2‑93 (MQ=255)
                                                                                              gATGCCGAAGATGGTAATTTGATTAATGCAAGCGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGaaa   >  1:2261059/1‑100 (MQ=255)
                                                                                              gATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGaaa   >  1:3999545/1‑100 (MQ=255)
                                                                                              gATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGaaa   >  1:5513056/1‑100 (MQ=255)
                                                                                              gATGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTAGGaagatcggaa   >  1:3538761/1‑92 (MQ=255)
                                                                                               aTGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGaaaa  >  1:1606140/1‑100 (MQ=255)
                                                                                               aTGCCGAAGATGGTAATTTGATTAATACAAGTGATGAAGATAAAACGACAGACGAAGAAGAAAAAGCACATTCGATTACAGCAATTTGGAATTTCGaaaa  >  1:5688862/1‑100 (MQ=255)
                                                                                                  |                                                                                                
AATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAATTTTAAACTTAGACGATGTCGAAGATGGTAATTTGATTAATGCAAGTGATGAAGATAAAACAACAGACGAAGAAGAAAAAGCACATTCAATTACCGCAATTTGGAATTTCGAAAA  >  NC_007790/777‑971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑