Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	298	G→T	A7S (GCT→TCC)	SAUSA300_RS14705 →	replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	298	0	G	T	100.0%	239.3 / NA	79	A7S (GCT→TCT)	SAUSA300_RS14705	replication protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (78/1);  total (78/1)

GTTTTGTAAATCAAAATTATGTCCGGTTTGTAATTGGAGACGTGCTATGAAAAATAGTTATCAAGCTCAAAAAGTGATTGAAAAAGTAATTAAGGAAAAGCCAAAAGCACGTTGGTTGTTTTTAACACTTTCAACAAAAAATGCGAT  >  NC_007790/234‑380                                                                 |                                                                                   cttctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAg                                                 >  1:4033750/9‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                               >  1:99043/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                               >  1:4453865/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                               >  1:4033924/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                               >  1:394625/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                               >  1:1443954/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                               >  1:3588118/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                               >  1:2412437/7‑100 (MQ=255)   gctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                               >  1:3748665/7‑100 (MQ=255)    ctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccg                                              >  1:3983070/6‑99 (MQ=255)    ctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccg                                              >  1:5569875/6‑99 (MQ=255)    ctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccg                                              >  1:1325676/6‑99 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccgca                                            >  1:4380953/4‑97 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:1084863/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:4598836/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:3980831/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:4844862/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:37198/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:3717605/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:5165880/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:3616043/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:5329854/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:3361949/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:3208911/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:5812605/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:932323/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:5832145/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:1884839/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:2633601/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:704044/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGCTTAAAGAAAAGCCgaa                                            >  1:1282400/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGAGTTTAAAGAAAAGCCgaa                                            >  1:3745117/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGAATGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:4018120/4‑100 (MQ=255)      ggcaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGCTCTATGAAAAATAGGTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:817120/4‑100 (MQ=255)      gataaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                                            >  1:2941922/4‑100 (MQ=255)                   aTGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAACGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggttg                               >  1:4800303/1‑100 (MQ=255)                    gtgccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgt                              >  1:2818388/7‑100 (MQ=255)                     gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGCAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                             >  1:26516/1‑100 (MQ=255)                     gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                             >  1:2111821/1‑100 (MQ=255)                     gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                             >  1:4088399/1‑100 (MQ=255)                     gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                             >  1:4209494/1‑100 (MQ=255)                     gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                             >  1:4435171/1‑100 (MQ=255)                     gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                             >  1:4454546/1‑100 (MQ=255)                      tCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGTATTCTCGTTGGTTGttt                            >  1:2924566/1‑100 (MQ=255)                       ccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGtttc                           >  1:6040936/4‑99 (MQ=255)                       ccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGtttc                           >  1:1310397/4‑99 (MQ=255)                            ttGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTaca                      >  1:1031837/1‑100 (MQ=255)                            ttGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTaca                      >  1:1903985/1‑100 (MQ=255)                             tGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTacat                     >  1:581826/1‑99 (MQ=255)                              gTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTacatt                    >  1:4934173/1‑98 (MQ=255)                                   tGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATcag               >  1:4141711/1‑99 (MQ=255)                                   tGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATcaa               >  1:910245/1‑100 (MQ=255)                                             cTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGc     >  1:273290/1‑100 (MQ=255)                                               aTGAAATATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:5580078/1‑98 (MQ=255)                                               aTGAAACATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGGATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcag   >  1:1980457/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGCAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:3703264/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGGATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:1211021/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATggca   >  1:2979799/1‑97 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:5621481/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:2055898/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:90541/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:224123/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:708058/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:2474026/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:2800209/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:5674075/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:4434220/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:5598425/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:1863920/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:5262388/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:1473751/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:3776960/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:1434987/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:4785851/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:4752449/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:4702177/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGcaa   >  1:4030799/1‑98 (MQ=255)                                               aTGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGCTGTTTCTTACATTATCAATACGAAATGcaa   >  1:85801/1‑98 (MQ=255)                                                tGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATCAATACGAAATGCAAt  <  1:2872536/100‑1 (MQ=255)                                                                 |                                                                                   GTTTTGTAAATCAAAATTATGTCCGGTTTGTAATTGGAGACGTGCTATGAAAAATAGTTATCAAGCTCAAAAAGTGATTGAAAAAGTAATTAAGGAAAAGCCAAAAGCACGTTGGTTGTTTTTAACACTTTCAACAAAAAATGCGAT  >  NC_007790/234‑380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑