Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	729	G→C	S150S (TCG→TCC)	SAUSA300_RS14705 →	replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	729	0	G	C	100.0%	223.0 / NA	82	S150S (TCG→TCC)	SAUSA300_RS14705	replication protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (74/8);  total (74/8)

TGACTATCGACCTGTTGCTAATGTTAAAGCGATCAAACCGAATAGAAAAGGCGATAAAGACATTGAATCGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGT  >  NC_007790/633‑825                                                                                                 |                                                                                                  tacttATCGACCTGTCGTAAATATTAAAGCGATCAAACC‑‑‑‑A‑AAAAGGAGATAAAGATATTGCGTCTGCTATCAAGGAGACATCAAAATATTCCGTTAAatc                                                                                           >  1:2957014/5‑100 (MQ=255)         gACCTGTCGTAAATATTAAAGCGATCAAACC‑‑‑‑A‑AAAAGGAGATAAAGATATTGCGTCTGCTATCAAGGAGACATCAAAATATTCCGTTAAATCATCTGact                                                                                   >  1:1477282/1‑98 (MQ=255)                                             taaaaGGAGATAAAGATATTGCGTCTGCTATCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGa                                                   >  1:5833852/2‑100 (MQ=255)                                              caaaGGAGATAAAGATATTGCGTCTGCTATCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGaa                                                  >  1:104/2‑100 (MQ=255)                                                    agATAAAGATATTGCGTCTGCTATCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAAttatc                                            <  1:4542502/99‑4 (MQ=255)                                                                   tataacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaag                             >  1:221725/6‑99 (MQ=255)                                                                    ataacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaagg                            >  1:890634/5‑98 (MQ=255)                                                                    ataacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaagg                            >  1:2862335/5‑98 (MQ=255)                                                                     taacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaaggt                           >  1:2750975/4‑97 (MQ=255)                                                                     gaccAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaaggt                           >  1:3737961/4‑97 (MQ=255)                                                                     gaacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaaggt                           >  1:5576103/4‑97 (MQ=255)                                                                     ctgctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAgg                           >  1:6018524/6‑100 (MQ=255)                                                                     ctgctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAgg                           >  1:2943434/6‑100 (MQ=255)                                                                     ctgctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAgg                           >  1:441617/6‑100 (MQ=255)                                                                     ctgctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAgg                           >  1:3210335/6‑100 (MQ=255)                                                                     ctgctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAgg                           >  1:1713272/6‑100 (MQ=255)                                                                      aacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaaggtt                          >  1:435161/3‑96 (MQ=255)                                                                      aacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaaggtt                          >  1:2484140/3‑96 (MQ=255)                                                                      aacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaaggtt                          >  1:5702444/3‑96 (MQ=255)                                                                       gctaTCAAGGTGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:5533489/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATGAAAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGt                          >  1:1362130/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:4017731/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:3267193/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:3393980/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:1148623/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:3945046/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:779373/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:426309/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:4370099/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:5603125/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:5213278/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:5367808/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:2945307/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:1595894/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:629766/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:899092/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                         >  1:2027631/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACAGATAGCTATG‑AAAAAAATCAAGAAATTATCAGTGTCCTAGAAAAAGGtt                         >  1:5883051/4‑100 (MQ=255)                                                                       gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGA‑CTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGttt                        >  1:2773697/4‑100 (MQ=255)                                                                       ctgcccAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGttt                        >  1:2356951/6‑100 (MQ=255)                                                                       acAATCAAAGAGACATCAAAATATTCCTTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTTAGTGATCTAG‑AAAAGGttt                        <  1:2712678/99‑1 (MQ=255)                                                                        gggggagggagaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGttt                        >  1:6015640/9‑100 (MQ=255)                                                                        cccgcAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTg                       >  1:4798793/5‑100 (MQ=255)                                                                          ctCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGGGATCTAGAAAAAGGTTTGt                      >  1:776831/2‑100 (MQ=255)                                                                          cgcAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTa                     >  1:5761228/3‑100 (MQ=255)                                                                          cccAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTa                     >  1:5887239/3‑100 (MQ=255)                                                                          aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATGAAAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTg                       >  1:2803011/1‑100 (MQ=255)                                                                          aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGt                      >  1:837156/1‑100 (MQ=255)                                                                          aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGt                      >  1:1091563/1‑100 (MQ=255)                                                                          aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGt                      >  1:5415962/1‑100 (MQ=255)                                                                          aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGt                      >  1:681172/1‑100 (MQ=255)                                                                          aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGt                      >  1:2336604/1‑100 (MQ=255)                                                                          aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGt                      >  1:3072070/1‑100 (MQ=255)                                                                          aTCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTCTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTa                     >  1:1087240/1‑100 (MQ=255)                                                                           ccAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGAAAAAgatgagat                     >  1:771387/2‑93 (MQ=255)                                                                           acagaaggaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTAt                    >  1:4196737/8‑100 (MQ=255)                                                                            cAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTAt                    >  1:2825272/1‑100 (MQ=255)                                                                            cAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATc                   <  1:5588955/100‑1 (MQ=255)                                                                            cAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCCAGaaaagatcggaaga                    >  1:5457938/1‑90 (MQ=255)                                                                            cAAAGAGACATCAAAATATTCCGTTAAATCATCTGACCTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGCTTGTATc                   >  1:2826308/1‑100 (MQ=255)                                                                             ccagagACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCg                  >  1:3232872/3‑100 (MQ=255)                                                                             atatgggggtCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCg                  >  1:2982748/10‑100 (MQ=255)                                                                              aaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACGAAAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCg                  >  1:1048700/1‑100 (MQ=255)                                                                              aaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACA‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGa                 >  1:2701390/1‑100 (MQ=255)                                                                                gagaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaa               >  1:2530701/1‑100 (MQ=255)                                                                                 tgaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaaa              >  1:4534128/2‑100 (MQ=255)                                                                                 agaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGaaa               >  1:489545/1‑100 (MQ=255)                                                                                 aaacatCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaaa              >  1:2843240/6‑100 (MQ=255)                                                                                  gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACGAAAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaaa              >  1:741493/1‑100 (MQ=255)                                                                                  gaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGaaaa              >  1:889749/1‑100 (MQ=255)                                                                                  cacatCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAAc             >  1:4276682/5‑100 (MQ=255)                                                                                    catCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGt           <  1:2112820/98‑1 (MQ=255)                                                                                       cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑ATAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATg        >  1:4093208/1‑100 (MQ=255)                                                                                       cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTaat        <  1:294743/100‑3 (MQ=255)                                                                                       cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATg        >  1:3142804/1‑100 (MQ=255)                                                                                       cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATg        >  1:1520127/1‑100 (MQ=255)                                                                                       cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTAt         <  1:4273866/100‑1 (MQ=255)                                                                                         aaaTATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGtt      <  1:1312032/100‑1 (MQ=37)                                                                                         aaaTATTCCGTTAAAGCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGtt      <  1:1792724/100‑1 (MQ=37)                                                                                             atTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGt  >  1:3731923/1‑100 (MQ=25)                                                                                             atTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGt  >  1:3561498/1‑100 (MQ=25)                                                                                             atTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGt  >  1:1238017/1‑100 (MQ=25)                                                                                                 |                                                                                                  TGACTATCGACCTGTTGCTAATGTTAAAGCGATCAAACCGAATAGAAAAGGCGATAAAGACATTGAATCGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGT  >  NC_007790/633‑825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑