Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	277	G→T	intergenic (–/‑3)	– / → SAUSA300_RS14705	–/replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	277	0	G	T	100.0%	151.3 / NA	52	intergenic (–/‑3)	–/SAUSA300_RS14705	–/replication protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (52/0);  total (52/0)

GTTTTGTAAATCAAAATTATGTCCGGTTTGTAATTGGAGACGTGCTATGAAAAATAGTTATCAAGCTCAAAAAGTGATTGAAAAAGTAATTAAGGAAAAGCCAAAAGCACGTTGGTTGTTTTTAACACTTTCAA  >  NC_007790/234‑367                                            |                                                                                           cttctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAg                                    >  1:4033750/9‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                  >  1:99043/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                  >  1:394625/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                  >  1:3588118/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                  >  1:1443954/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                  >  1:4033924/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                  >  1:4453865/7‑100 (MQ=255)   tctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                  >  1:2412437/7‑100 (MQ=255)   gctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGcc                                  >  1:3748665/7‑100 (MQ=255)    ctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccg                                 >  1:5569875/6‑99 (MQ=255)    ctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccg                                 >  1:1325676/6‑99 (MQ=255)    ctggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccg                                 >  1:3983070/6‑99 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGccgca                               >  1:4380953/4‑97 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:5329854/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:5165880/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:4844862/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:4598836/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:5812605/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:5832145/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:704044/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:3980831/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:932323/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:1084863/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:37198/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:3717605/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:3616043/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:3361949/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:1884839/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:3208911/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:2633601/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGCTTAAAGAAAAGCCgaa                               >  1:1282400/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGAGTTTAAAGAAAAGCCgaa                               >  1:3745117/4‑100 (MQ=255)      ggtaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGAATGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:4018120/4‑100 (MQ=255)      ggcaaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGCTCTATGAAAAATAGGTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:817120/4‑100 (MQ=255)      gataaTCAAAGTTATGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCgaa                               >  1:2941922/4‑100 (MQ=255)                   aTGTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAACGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCgttggttg                  >  1:4800303/1‑100 (MQ=255)                    gtgccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgt                 >  1:2818388/7‑100 (MQ=255)                     gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGCAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                >  1:26516/1‑100 (MQ=255)                     gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                >  1:4454546/1‑100 (MQ=255)                     gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                >  1:4435171/1‑100 (MQ=255)                     gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                >  1:2111821/1‑100 (MQ=255)                     gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                >  1:4209494/1‑100 (MQ=255)                     gTCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGgttgtt                >  1:4088399/1‑100 (MQ=255)                      tCCAGTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGTATTCTCGTTGGTTGttt               >  1:2924566/1‑100 (MQ=255)                       ccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGtttc              >  1:6040936/4‑99 (MQ=255)                       ccagTTTGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGtttc              >  1:1310397/4‑99 (MQ=255)                            ttGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTaca         >  1:1903985/1‑100 (MQ=255)                            ttGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTaca         >  1:1031837/1‑100 (MQ=255)                             tGTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTacat        >  1:581826/1‑99 (MQ=255)                              gTAATTGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTacatt       >  1:4934173/1‑98 (MQ=255)                                   tGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATcag  >  1:4141711/1‑99 (MQ=255)                                   tGGAGACGTTCTATGAAAAATAGTTATCAATCCCAAAAAGTGATTGAAGAAGTGTTTAAAGAAAAGCCGAATTCTCGTTGGTTGTTTCTTACATTATcaa  >  1:910245/1‑100 (MQ=255)                                            |                                                                                           GTTTTGTAAATCAAAATTATGTCCGGTTTGTAATTGGAGACGTGCTATGAAAAATAGTTATCAAGCTCAAAAAGTGATTGAAAAAGTAATTAAGGAAAAGCCAAAAGCACGTTGGTTGTTTTTAACACTTTCAA  >  NC_007790/234‑367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑