Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007790	561	A→G	A94A (GCA→GCG)	SAUSA300_RS14705 →	replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007790	561	0	A	G	100.0%	26.7 / NA	12	A94A (GCA→GCG)	SAUSA300_RS14705	replication protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/2);  total (10/2)

TTGTTGGATTTATGCGTTCAACAGAAGTTACCGTTAATAAAAATGACGGTAGTTATAATCAGCACATGCATGTTTTGTTATGTGTTGAAAATGCATATTTTAGAAAAAAAGAGAATTATATAACTCAAGAAGAATGGGTTAATTTAT  >  NC_007790/467‑613                                                                                               |                                                     taattgGCTTTATGCATTCAACTGAAGTAACAGTTAATAAAAATGACGGTAGTTATAATCAACATATGCACGTTTTGTTGTGTGTTGAAAATGCGTAttg                                                 <  1:2669878/97‑2 (MQ=255)  atctgGCTTTATGCATTCAACTGAAGTAACAGTTAATAAAAATGACGGTAGTTATAATCAACATATGCACGTTTTGTTGTGTGTTGAAAATGCGTAtttt                                                >  1:117719/4‑100 (MQ=255)   attgGCTTTATGCATTCAACTGAAGTAACAGTTAATAAAAATGACGGTAGTTATAATCAACATATGCACGTTTTGTTGTGTGTTGAAAATGCGTATTTTa                                               >  1:3714068/2‑100 (MQ=255)     tgGCTTTATGCATTCAACTGAAGTAACAGTTAATAAATATGACGGTAGTTATAATCAACATATGCACGTTTTGTTGTGTGTTGAAAATGCGTATTTTAGa                                             >  1:2329207/1‑100 (MQ=255)     tgGCTTTATGCATTCAACTGAAGTAACAGTTAATAAAAATGACGGTAGTTATAATCAACATATGCACGTTTTGTTGTGTGTTGAAAATGCGTATTTTAGa                                             >  1:2796635/1‑100 (MQ=255)      ggctttATGCATTCAACTGAAGTAACAGTTAATAAAAATGACGGTAGTTATAATCAACATATGCACGTTTTGTTGTGTGTTGAAAATGCGTATTTTAGaa                                            >  1:3346175/4‑100 (MQ=255)        ctttATGCATTCAACTGAAGTAACAGTTAATAAAAATGACGGTAGTTATAATCAACATATGCACGTTTTGTTGTGTGTTGAAAATGCGTATTTTAGaaag                                          <  1:3057607/99‑2 (MQ=255)           tATGCATTCAACTGAAGTAACAGTTAATAAAAATGACGGTAGTTATAATCAACATATGCACGTTTTGTTGTGTGTTGAAAATGCGTATTTTAgaaagaaa                                       >  1:670059/1‑100 (MQ=255)                                           ctGACGGTAGTTATAATCAACATATGCACGTTTTGTTGTGTGTTGAAAATGCGTATTTTAGAAAGAAA‑‑‑AAATATATTACACAAACAGAATTGGTTGAttt    >  1:3522059/2‑100 (MQ=255)                                           aTGACGGTAGTTATAATCAACATATGCACGTTTTGTTGTGTGTTGAAAATGCGTATTTTAGAAAGAAA‑‑‑AAATATATTACACAAACAGAATTGGTTGAttt    >  1:6019266/1‑100 (MQ=255)                                            tGACGGTAGTTATAATCAACATATGCACGTTTTGTTGTGTGTTGAAAATGCGTATTTTAGAAAGAAA‑‑‑AAATATATTACACAAACAGAATTGGTTGATTTa   >  1:5942142/1‑100 (MQ=255)                                             gACGGTAGTTATAATCAACATATGCACGTTTTGTTGTGTGTTGAAAATGCGTATTTTAGAAAGAAA‑‑‑AAATATATTACACAAACAGAATTGGTTGATTTAt  >  1:4278829/1‑100 (MQ=255)                                                                                               |                                                     TTGTTGGATTTATGCGTTCAACAGAAGTTACCGTTAATAAAAATGACGGTAGTTATAATCAGCACATGCATGTTTTGTTATGTGTTGAAAATGCATATTTTAGAAAAAAAGAGAATTATATAACTCAAGAAGAATGGGTTAATTTAT  >  NC_007790/467‑613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑