Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077907580AG27.9% 232.8 / 105.3 197D160G (GAT→GGT) SAUSA300_RS14705replication protein
Reads supporting (aligned to +/- strand):  ref base A (133/9);  new base G (45/10);  total (178/19)
Fisher's exact test for biased strand distribution p-value = 1.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.62e-02

Only 100 of 270 total aligned reads displayed.

AAAAGGCGATAAAGACATTGAATCGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAAT  >  NC_007790/678‑857
                                                                                |                                                                                                    
                      ataacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaagg                                                            >  1:890634/5‑98 (MQ=255)
                       ctgctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAgg                                                           >  1:2943434/6‑100 (MQ=255)
                       ctgctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAgg                                                           >  1:1713272/6‑100 (MQ=255)
                       ctgctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAgg                                                           >  1:441617/6‑100 (MQ=255)
                        aacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaaggtt                                                          >  1:435161/3‑96 (MQ=255)
                        aacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaaggtt                                                          >  1:2484140/3‑96 (MQ=255)
                        aacAATCAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAGaaaaggtt                                                          >  1:5702444/3‑96 (MQ=255)
                         gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATGAAAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGt                                                          >  1:1362130/4‑100 (MQ=255)
                         gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                                                         >  1:5213278/4‑100 (MQ=255)
                         gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                                                         >  1:1148623/4‑100 (MQ=255)
                         gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                                                         >  1:3393980/4‑100 (MQ=255)
                         gctaTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGtt                                                         >  1:2027631/4‑100 (MQ=255)
                         ctgcccAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGttt                                                        >  1:2356951/6‑100 (MQ=255)
                            ctCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGGGATCTAGAAAAAGGTTTGt                                                      >  1:776831/2‑100 (MQ=255)
                            cccAAAGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTa                                                     >  1:5887239/3‑100 (MQ=255)
                            aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATGAAAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTg                                                       >  1:2803011/1‑100 (MQ=255)
                            aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGt                                                      >  1:681172/1‑100 (MQ=255)
                            aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGt                                                      >  1:3072070/1‑100 (MQ=255)
                            aTCAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGt                                                      >  1:837156/1‑100 (MQ=255)
                             acagaaggaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTAt                                                    >  1:4196737/8‑100 (MQ=255)
                              cAAGGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTAt                                                    >  1:2825272/1‑100 (MQ=255)
                                aaGAGACATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACGAAAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCg                                                  >  1:1048700/1‑100 (MQ=255)
                                  gagaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaa                                               >  1:2530701/1‑100 (MQ=255)
                                   tgaCATCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaaa                                              >  1:4534128/2‑100 (MQ=255)
                                   aaacatCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGaaaa                                              >  1:2843240/6‑100 (MQ=255)
                                    cacatCAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAAc                                             >  1:4276682/5‑100 (MQ=255)
                                         cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATg                                        >  1:3142804/1‑100 (MQ=255)
                                         cAAAATATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATg                                        >  1:1520127/1‑100 (MQ=255)
                                           aaaTATTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGtt                                      <  1:1312032/100‑1 (MQ=37)
                                               atTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGt                                  >  1:3561498/1‑100 (MQ=25)
                                               atTCCGTTAAATCATCTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGt                                  >  1:3731923/1‑100 (MQ=25)
                                                    ggggcctcatcTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtgg                             >  1:4377072/7‑100 (MQ=25)
                                                      ttccgatcttGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggt                            <  1:3754809/91‑1 (MQ=21)
                                                      tAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggt                            <  1:5398156/100‑1 (MQ=25)
                                                      tAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggt                            <  1:5911955/100‑1 (MQ=25)
                                                      tAAATCATCTGACTTTTTAACTGATAGCGATG‑AAAAAAATCAAGAAATTATCAGTGATCTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggt                            <  1:107290/100‑1 (MQ=25)
                                                       aggtcatcTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTAtggtgg                          <  1:5591435/97‑1 (MQ=25)
                                                        cttcctcTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGa                         >  1:4084710/6‑100 (MQ=25)
                                                              cTGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACtt                   >  1:4902097/1‑100 (MQ=25)
                                                               tGACTTTTTAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTa                  >  1:2735786/1‑100 (MQ=25)
                                                                gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                 >  1:5469410/4‑100 (MQ=25)
                                                                gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                 >  1:3484980/4‑100 (MQ=25)
                                                                gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                 >  1:4485980/4‑100 (MQ=25)
                                                                gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                 >  1:3331396/4‑100 (MQ=25)
                                                                gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                 >  1:739841/4‑100 (MQ=25)
                                                                gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                 >  1:5761198/4‑100 (MQ=25)
                                                                gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                 >  1:2705522/4‑100 (MQ=25)
                                                                gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                 >  1:2754568/4‑100 (MQ=25)
                                                                gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTaa                 >  1:2105153/4‑100 (MQ=25)
                                                                gactttttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGACTACTTaa                 >  1:2620762/4‑100 (MQ=25)
                                                                    gctccccTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaa             >  1:471303/7‑100 (MQ=25)
                                                                    agttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGCGGATTACTTAAAGaa             >  1:3338585/3‑100 (MQ=21)
                                                                     tttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaa            >  1:2641940/1‑100 (MQ=25)
                                                                      ttAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaa           >  1:4151814/1‑100 (MQ=25)
                                                                       tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag          >  1:5989366/1‑99 (MQ=25)
                                                                       tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag          >  1:5448502/1‑99 (MQ=25)
                                                                       tAACTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag          >  1:5530561/1‑99 (MQ=25)
                                                                       ggccTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAGCGTATGTTAAGTTATGGTGGATTACTTAAAGaaaag          >  1:5102536/4‑99 (MQ=255)
                                                                        taCTGATGACGACA‑AAAAAAATCAAGAAACTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc         >  1:557317/2‑98 (MQ=21)
                                                                        gaCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc         >  1:5041845/2‑98 (MQ=25)
                                                                        cgcTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc         >  1:4883555/3‑98 (MQ=25)
                                                                        agcTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc         >  1:2977837/3‑98 (MQ=25)
                                                                        aaCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGTAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc         >  1:1275808/1‑98 (MQ=25)
                                                                        aaCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGaaaagc         >  1:613247/1‑98 (MQ=25)
                                                                         aCTGATGACGACG‑AAAAAAATCAGGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa        >  1:1395574/1‑100 (MQ=21)
                                                                         aCTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCa        >  1:4776408/1‑100 (MQ=25)
                                                                          cTGTTGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt       >  1:1010559/1‑100 (MQ=21)
                                                                          cTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt       >  1:458975/1‑100 (MQ=25)
                                                                          cTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt       >  1:3809079/1‑100 (MQ=25)
                                                                          cTGATGACGACG‑AAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCAt       >  1:5190448/1‑100 (MQ=25)
                                                                           tgatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATa      >  1:1873540/1‑100 (MQ=25)
                                                                            ggtgacgacg‑aTAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:2999097/3‑100 (MQ=21)
                                                                            gctgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:4012274/3‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAGAGAAAAGCATaa     >  1:3240024/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:5149247/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:6019163/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:6048014/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:2074606/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:1229479/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:764587/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:4861944/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:1110078/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:4978720/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:803382/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:2320075/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:1453472/1‑100 (MQ=21)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGGTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:3697898/1‑100 (MQ=255)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGCTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:5578557/1‑100 (MQ=255)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTAGTATCGAAAACGAATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:3928237/1‑100 (MQ=255)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGCTTGTATCGAAAACGACTGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:4266193/1‑100 (MQ=255)
                                                                            gatgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGATTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaa     >  1:2377609/1‑100 (MQ=21)
                                                                             atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATagg    <  1:2492621/100‑3 (MQ=21)
                                                                             atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa    >  1:2202386/1‑100 (MQ=21)
                                                                             atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa    >  1:5864369/1‑100 (MQ=21)
                                                                             atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa    >  1:5775599/1‑100 (MQ=21)
                                                                             atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa    >  1:1839159/1‑100 (MQ=21)
                                                                             atgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaa    >  1:4224464/1‑100 (MQ=21)
                                                                              tgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa   >  1:2141456/1‑100 (MQ=21)
                                                                              tgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa   >  1:3428990/1‑100 (MQ=21)
                                                                              tgacgacg‑aAAAAAATCAAGAAATTTTAAGTGATCTAG‑AAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTACTTAAAGAAAAGCATaaaa   >  1:5302826/1‑100 (MQ=21)
                                                                                |                                                                                                    
AAAAGGCGATAAAGACATTGAATCGGCAATCAAAGAGACCTCAAAATATTCGGTTAAATCATCTGATTTTTTAACTGATGATGATG‑AAAAAAATCAAGAAATTGTAAGTGATTTAGAAAAAGGTTTGTATCGAAAACGTATGTTAAGTTATGGTGGATTGCTTAAACAAAAACATAAAAT  >  NC_007790/678‑857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑