Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	376,057	C→T	D258N (GAC→AAC)	SAUSA300_RS01715 ←	NADH‑dependent flavin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	376,057	0	C	T	100.0%	66.9 / NA	23	D258N (GAC→AAC)	SAUSA300_RS01715	NADH‑dependent flavin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/12);  total (11/12)

TTTGGTTAACTGGTCGACCGAAATGATCACCTGGTGTACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTCTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGTTGCT  >  NC_007793/375961‑376145                                                                                                 |                                                                                         tttGGTTAACTGGTCGACCGAAATGATCACCTGGTGTACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTgc                                                                                       <  1:5929506/100‑2 (MQ=255)       tagcTGGTCGACCGAAATGATCACCTGGTGTACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTc                                                                                 >  1:2582474/4‑100 (MQ=255)            ggTCGACCGAAATGATCACCTGGTGTACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGa                                                                            >  1:3997945/1‑100 (MQ=255)              ctgACCGAAATGATCACCTGGTGTACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGAcc                                                                          >  1:4012415/3‑100 (MQ=255)               cGACCGAAATGATCACCTGGTGTACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGAccc                                                                         >  1:4985011/1‑100 (MQ=255)                   cGAAATGATCACCTGGTGTACGAGACGTATTTTGATAAAGATGGCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCactg                                                                     <  1:2659301/100‑4 (MQ=255)                                         agaCGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCa                                               <  1:5525288/100‑1 (MQ=255)                                              tATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGt                                          <  1:2155957/100‑1 (MQ=255)                                                                gACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGcc                        <  1:5608560/100‑1 (MQ=255)                                                                ccccccAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGcc                        >  1:2857440/3‑100 (MQ=255)                                                                  ccccAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCctc                      <  1:5896349/100‑1 (MQ=255)                                                                  ccccAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCctc                      >  1:4933943/1‑100 (MQ=255)                                                                   cccAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCctct                     >  1:5697585/1‑100 (MQ=255)                                                                   cccAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCctat                     <  1:6102601/100‑3 (MQ=255)                                                                    ccAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCctcaa                    <  1:5987210/100‑3 (MQ=255)                                                                    ccAGCTAGCGATTGCTAAGTATTGGATGCTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTg                    <  1:1291227/100‑1 (MQ=255)                                                                     cagtcaGCGATTGCTAAGTATTGGATGTTTGAAACGCCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTgg                   >  1:5475923/6‑100 (MQ=255)                                                                     cAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTgg                   >  1:2878065/1‑100 (MQ=255)                                                                     cAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTgg                   >  1:1788658/1‑100 (MQ=255)                                                                          aGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTttct              >  1:1329621/1‑100 (MQ=255)                                                                                  cTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGt      <  1:5027079/100‑1 (MQ=255)                                                                                    aaGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGTTg    <  1:5312319/100‑1 (MQ=255)                                                                                      gTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGTTGCt  <  1:3204097/100‑1 (MQ=255)                                                                                                 |                                                                                         TTTGGTTAACTGGTCGACCGAAATGATCACCTGGTGTACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTCTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGTTGCT  >  NC_007793/375961‑376145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑