Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	245,065	C→A	intergenic (‑74/‑135)	SAUSA300_RS01085 ← / → SAUSA300_RS01090	FMN‑dependent NADH‑azoreductase/peptidase M23

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	245,065	0	C	A	100.0%	21.2 / NA	8	intergenic (‑74/‑135)	SAUSA300_RS01085/SAUSA300_RS01090	FMN‑dependent NADH‑azoreductase/peptidase M23
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (1/7);  total (1/7)

GCCATTTGAAAATTCTCCTCTGTTTCTGTTATTTTCTTAAGTATAATTATTATACTCGATATAAAATTTAATATCAATCAAAATATTCAAATTACCATCATTTTCTTCATCTATATTTGGCAGTACTACTAAAGTATGAGTGCATTTAATTATGAAATAGTT  >  NC_007793/244987‑245148                                                                               |                                                                                    gCCATTTGAAAATTCTCCTCTGTTTCTGTTATTTTCTTAAGTATAATTATTATACTCGATATAAAATTTAATATCAATAAAAATATTCAAATTACcatca                                                                <  1:1872558/100‑1 (MQ=255)                      gtttctgttATTTTCTTAAGTATAATTATTATACTCGATATAAAATTTAATATCAATAAAAATATTCAAATTACCATCATTTTCTTCATCTATATTTGGc                                           <  1:3439273/100‑1 (MQ=255)                      gtttctgttATTTTCTTAAGTATAATTATTATACTCGATATAAAATTTAATATCAATAAAAATATTCAAATTACCATCATTTTCTTCATCTATATTTGGc                                           <  1:5993828/100‑1 (MQ=255)                      gtttctgttATTTTCTTAAGTATAATTATTATACTCGATATAAAATTTAATATCAATAAAAATATTCAAATTACCATCATTTTCTTCATCTATATTTGGc                                           <  1:6197402/100‑1 (MQ=255)                        ttctgttATTTTCTTAAGTATAATTATTATACTCGATATAAAATTTAATATCAATAAAAATATTCAAATTACCATCATTTTCTTCATCTATATTTGGCAg                                         <  1:16981/100‑1 (MQ=255)                         tctgttATTTTCTTAAGTATAATTATTATACTCGATATAAAATTTAATATCAATAAAAATATTCAAATTACCATCATTTTCTTCATCTATATTTGGCAGt                                        <  1:2667991/100‑1 (MQ=255)                         tctgttATTTTCTTAAGTATAATTATTATACTCGATATAAAATTTAATATCAATAAAAATATTCAAATTACCATCATTTTCTTCATCTATATTTGGCAGt                                        <  1:2728886/100‑1 (MQ=255)                                                               aaaaTTTAATATCAATAAAAATATTCAAATTACCATCATTTTCTTCATCTATATTTGGCAGTACTACTAAAGTATGAGTGCATTTAATTATGAAATAGtt  >  1:3347855/1‑100 (MQ=255)                                                                               |                                                                                    GCCATTTGAAAATTCTCCTCTGTTTCTGTTATTTTCTTAAGTATAATTATTATACTCGATATAAAATTTAATATCAATCAAAATATTCAAATTACCATCATTTTCTTCATCTATATTTGGCAGTACTACTAAAGTATGAGTGCATTTAATTATGAAATAGTT  >  NC_007793/244987‑245148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑