Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	76,319	C→T	A17A (GCC→GCT)	SAUSA300_RS00345 →	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	76,319	0	C	T	93.1%	107.1 / 0.7	58	A17A (GCC→GCT)	SAUSA300_RS00345	transposase
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (7/47);  total (9/49)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-01

AAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCG  >  NC_007793/76225‑76414                                                                                               |                                                                                                aaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                                                                            <  1:2967658/100‑1 (MQ=32)                                                          gATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACg                                   <  1:208621/100‑1 (MQ=17)                                                             ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgg                                <  1:1305875/100‑1 (MQ=17)                                                              taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggt                               <  1:4360896/100‑1 (MQ=17)                                                              taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggt                               <  1:3382833/100‑1 (MQ=17)                                                              taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggt                               >  1:3272674/1‑100 (MQ=17)                                                              taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggt                               <  1:4851102/100‑1 (MQ=17)                                                              taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggt                               <  1:571103/100‑1 (MQ=17)                                                              taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggg                               <  1:5153223/100‑2 (MQ=17)                                                              taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggc                               <  1:5434473/100‑2 (MQ=17)                                                              taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgcg                               <  1:5752216/100‑3 (MQ=17)                                                              aaaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggt                               >  1:270589/2‑100 (MQ=17)                                                               aaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTTCTATCTAAGATATGCATTGAGTTATCGTGTTATATCTGAAATATTAAGGGAACgtggtg                              <  1:2743422/100‑1 (MQ=255)                                                               aaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATTGTGATATATCTGAAATATTAAGGGAACgtggtg                              >  1:5126565/1‑100 (MQ=255)                                                               aaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggtg                              <  1:4089008/100‑1 (MQ=17)                                                               aaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggtg                              <  1:5743813/100‑1 (MQ=17)                                                               aaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggtg                              <  1:1521004/100‑1 (MQ=17)                                                               aaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggtg                              >  1:4992168/1‑100 (MQ=32)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGTTATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:3363738/100‑1 (MQ=12)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggtgg                             <  1:303990/100‑2 (MQ=17)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgggta                             <  1:644375/100‑4 (MQ=12)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:5216920/100‑1 (MQ=17)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:5668262/100‑1 (MQ=17)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:4833222/100‑1 (MQ=17)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:600857/100‑1 (MQ=17)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:4297084/100‑1 (MQ=17)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:4150240/100‑1 (MQ=17)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:1204234/100‑1 (MQ=17)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:4011093/100‑1 (MQ=17)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:1134671/100‑1 (MQ=17)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:3090941/100‑1 (MQ=17)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:3309819/100‑1 (MQ=17)                                                                aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                             <  1:3415491/100‑1 (MQ=17)                                                                 aCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgtg                            <  1:922632/100‑2 (MQ=17)                                                                 aCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                            <  1:4738863/100‑1 (MQ=17)                                                                 aCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                            <  1:1421987/100‑1 (MQ=17)                                                                 aCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                            <  1:5692981/100‑1 (MQ=17)                                                                 aCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                            <  1:199597/100‑1 (MQ=17)                                                                 aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                            <  1:4334572/100‑1 (MQ=32)                                                                 aCAATTTAACAAGAATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgtg                            <  1:1388618/100‑2 (MQ=255)                                                                  cAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTag                           <  1:407015/100‑2 (MQ=17)                                                                  cAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaa                           <  1:1734429/100‑1 (MQ=17)                                                                   aaTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaag                          <  1:3903814/100‑2 (MQ=17)                                                                         aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtca                    >  1:1578454/1‑100 (MQ=18)                                                                           aaGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatc                  <  1:4985052/100‑1 (MQ=18)                                                                                 gTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCaa            >  1:5228943/1‑100 (MQ=18)                                                                                   tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACg          >  1:4576059/1‑100 (MQ=32)                                                                                     tctcTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGt        >  1:5005924/4‑100 (MQ=12)                                                                                       aCTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt      <  1:5586135/100‑1 (MQ=17)                                                                                       aCTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt      <  1:2432066/100‑1 (MQ=17)                                                                                       aCTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt      <  1:2868129/100‑1 (MQ=17)                                                                                       aCTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt      <  1:6161324/100‑1 (MQ=17)                                                                                       aCTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt      <  1:350354/100‑1 (MQ=17)                                                                                       aCTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt      <  1:75056/100‑1 (MQ=17)                                                                                       aCTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt      <  1:4304184/100‑1 (MQ=17)                                                                                        cTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTa     <  1:2716940/100‑1 (MQ=17)                                                                                         tGTAGCTGTTGGTTTCTATCTAAGATATGCATTTAGTTAACGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTag    <  1:2004645/100‑2 (MQ=11)                                                                                         tGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTag    <  1:5469457/100‑2 (MQ=17)                                                                                           tAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCg  >  1:2879779/1‑100 (MQ=17)                                                                                               |                                                                                                AAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCG  >  NC_007793/76225‑76414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑