Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,786,770	T→A	intergenic (‑178/‑80)	SAUSA300_RS14315 ← / → SAUSA300_RS14320	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,786,770	0	T	A	100.0%	149.9 / NA	47	intergenic (‑178/‑80)	SAUSA300_RS14315/SAUSA300_RS14320	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (47/0);  total (47/0)

ACTAATTGAAGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTTAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAGGCGTTATATTTG  >  NC_007793/2786704‑2786852                                                                   |                                                                                   acatcatcaaGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAattatt                                                   >  1:3889999/9‑100 (MQ=255)       gccaGTACACAAAAAACAATGAAAATATTAATATCGCCCACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCtt                                             >  1:711434/4‑100 (MQ=255)       cacggTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCtt                                             >  1:2365593/5‑100 (MQ=255)        gtaGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTc                                            >  1:5718428/3‑100 (MQ=255)        ccaGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTc                                            >  1:3707719/3‑100 (MQ=255)         caGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCa                                           >  1:6011637/2‑100 (MQ=255)          tgTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:3048610/2‑100 (MQ=255)          ggTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:5095820/2‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:5012631/1‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:4475191/1‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:4352977/1‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:5296762/1‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:5641308/1‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:3054104/1‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:2793750/1‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:25299/1‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:781277/1‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:1565272/1‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:165425/1‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:1738413/1‑100 (MQ=255)          aGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCAc                                          >  1:2675857/1‑100 (MQ=255)          aGTACAC‑AAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACg                                         >  1:5581768/1‑100 (MQ=255)           gTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACg                                         >  1:5722647/1‑100 (MQ=255)           gTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACg                                         >  1:1455788/1‑100 (MQ=255)           gTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACg                                         >  1:1360304/1‑100 (MQ=255)           gTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACg                                         >  1:1018622/1‑100 (MQ=255)           gTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACg                                         >  1:5013751/1‑100 (MQ=255)            cacacAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGt                                        >  1:331654/2‑100 (MQ=255)             acacAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTa                                       >  1:5607382/1‑100 (MQ=255)             acacAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTa                                       >  1:4315945/1‑100 (MQ=255)             acacAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGACTATGGCAATATTGAAATAAATTATTTAGCTTCACGTa                                       >  1:228643/1‑100 (MQ=255)                caAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCa                                    >  1:1263972/1‑100 (MQ=255)                                      actCGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAg              >  1:1320228/3‑100 (MQ=255)                                       acggCCTACTTTTCTAATAATTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAAAATGGAga             >  1:1435848/4‑99 (MQ=255)                                       aaccccTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACACGGAga             >  1:5677449/5‑99 (MQ=255)                                         tgCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCTACTACTTTTCATTAACATGGAgatc           >  1:3288912/2‑97 (MQ=255)                                         tgCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAGGCg           >  1:2246560/2‑100 (MQ=255)                                         cGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAGGCg           >  1:3605984/1‑100 (MQ=255)                                         cGCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAGGCg           >  1:284557/1‑100 (MQ=255)                                          gCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAGGCGt          >  1:3627477/1‑100 (MQ=255)                                          gCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAGGCGt          >  1:3384074/1‑100 (MQ=255)                                          gCCTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAGGCGt          >  1:1800535/1‑100 (MQ=255)                                           ccTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAGGCGtt         >  1:5425516/1‑100 (MQ=255)                                            cTACTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAGGCGTta        >  1:3527511/1‑100 (MQ=255)                                              aCTTTTCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAGGCGTtata      >  1:5417602/1‑100 (MQ=255)                                              aCTTTTCTAATATTTATCTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGaagatcggaa      >  1:5593440/1‑91 (MQ=255)                                                  ttCTAATATTTATTTTTAAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAGGCGTTATATTTg  >  1:4957805/1‑100 (MQ=255)                                                                   |                                                                                   ACTAATTGAAGTACACAAAAAACAATGAAAATATTAATATCGCCTACTTTTCTAATATTTATTTTTTAAAATGATTATGGCAATATTGAAATAAATTATTTAGCTTCACGTATCAACTACTTTTCATTAACATGGAGGCGTTATATTTG  >  NC_007793/2786704‑2786852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑