Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,990,389	C→T	G479G (GGG→GGA)	SAUSA300_RS09895 ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,990,389	0	C	T	100.0%	42.9 / NA	16	G479G (GGG→GGA)	SAUSA300_RS09895	ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/15);  total (1/15)

CCCCTTGATTGATTTCAAGATTGATATCTTTTAAAACTTCATTATCTCCAAAAACTTTATTAAGATTGTTTATTTTAATCACTGGCATTCAATCTCCCTTCAATCATGTTCATAATGCGTGTAAGTACAAATGTTAAGACAAAGTATAATGCTGCTGCCACTATTAATGGTGTGAATGGGTCAAATG  >  NC_007793/1990294‑1990480                                                                                                |                                                                                            ccccTTGATTGATTTCAAGATTGATATCTTTTAAAACTTCATTATCTCCAAAAACTTTATTAAGACTGTTTATTTTAATCACTGGCATTCAATCTTCCtt                                                                                         >  1:5132774/1‑100 (MQ=255)                     ttGATATCTTTTAAAACTTCATTATCTCCAAAAACTTTATTAAGATTGTTTATTTTAATCACTGGCATTCAATCTTCCTTCAATCATGTTCATAATGCgt                                                                     <  1:5125913/100‑1 (MQ=255)                       gATATCTTTTAAAACTTCATTATCTCCAAAAACTTTATTAAGATTGTTTATTTTAATCACTGGCATTCAATCTTCCTTCAATCATGTTCATAATGCgtgt                                                                   <  1:4555528/100‑1 (MQ=255)                       gATATCTTTTAAAACTTCATTATCTCCAAAAACTTTATTAAGATTGTTTATTTTAATCACTGGCATTCAATCTTCCTTCAATCATGTTCATAATGCgtgt                                                                   <  1:5847011/100‑1 (MQ=255)                        atatCTTTTAAAACTTCATTATCTCCAAAAACTTTATTAAGATTGTTTATTTTAATCACTGGCATTCAATCTTCCTTCAATCATGTTCATAATGCgtgga                                                                  <  1:3373467/100‑3 (MQ=255)                        atatCTTTTAAAACTTCATTATCTCCAAAAACTTTATTAAGATTGTTTATTTTAATCACTGGCATTCAATCTTCCTTCAATCATGTTCATAATGCGTGTa                                                                  <  1:2190065/100‑1 (MQ=255)                        atatCTTTTAAAACTTCATTATCTCCAAAAACTTTATTAAGATTGTTTATTTTAATCACTGGCATTCAATCTTCCTTCAATCATGTTCATAATGCGTGTa                                                                  <  1:5955770/100‑1 (MQ=255)                        atatCTTTTAAAACTTCATTATCTCCAAAAACTTTATTAAGATTGTTTATTTTAATCACTGGCATTCAATCTTCCTTCAATCATGTTCATAATGCGTGTa                                                                  <  1:6189616/100‑1 (MQ=255)                                                         ttattaAGATTGTTTATTTTAATCACTGGCATTCAATCTTCCTTCAATCATGTTCATAATGCGTGTAAGTACAAATGTTAAGACAAAGTATAAtgctgca                                 <  1:1154305/100‑2 (MQ=255)                                                            ttaAGATTGTTTATTTTAATCACTGGCATTCAATCTTCCTTCAATCATGTTCATAATGCGTGTAAGTACAAATGTTAAGACAAAGTATAATGCTGCTGcc                              <  1:4744970/100‑1 (MQ=255)                                                                gATTGTTTATTTTAATCACTGGCATTCAATCTTCCTTCAATCATGTTCATAATGCGTGTAAGTACAAATGTTAAGACAAAGTATAATGCTGCTGCCAcaa                          <  1:503621/100‑3 (MQ=255)                                                                  ttGTTTATTTTAATCACTGGCATTCAATCTTCCTTCAATCATGTTCATAATGCGTGTAAGTACAAATGTTAAGACAAAGTATAATGCTGCTGCCACTAtt                        <  1:5598057/100‑1 (MQ=255)                                                                           ttAATCACTGGCATTCAATCTTCCTTCAATCATGTTCATAATGCGTGTAAGTACAAATGTTAAGACAAAGTATAATGCTGCTGCCACTATTAATGGtgtg               <  1:961866/100‑1 (MQ=255)                                                                                  cTGGCATTCAATCCTCCTTCAATCATGTTCATAATGCGTGTAAGTACAAATGTTAAGACAAAGTATAATGCTGCTGCCACTATTAATGGTGTGAATGGGt        <  1:551445/100‑1 (MQ=255)                                                                                    ggCATTCAATCTTCCTTCAATCATGTTCATAATGCGTGTAAGTACAAATGTTAAGACAAAGTATAATGCTGCTGCCACTATTAATGGTGTGAATGGGTCa      <  1:4621290/100‑1 (MQ=255)                                                                                        ttCAATCTTCCTTCAATCATGTTCATAATGCGTGTAAGTACAAATGTTAAGACAAAGTATAATGCTGCTGCCACTATTAATGGTGTGAATGGGTCAAATg  <  1:5123370/100‑1 (MQ=255)                                                                                                |                                                                                            CCCCTTGATTGATTTCAAGATTGATATCTTTTAAAACTTCATTATCTCCAAAAACTTTATTAAGATTGTTTATTTTAATCACTGGCATTCAATCTCCCTTCAATCATGTTCATAATGCGTGTAAGTACAAATGTTAAGACAAAGTATAATGCTGCTGCCACTATTAATGGTGTGAATGGGTCAAATG  >  NC_007793/1990294‑1990480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑