Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,846,875	G→A	S10F (TCT→TTT)	SAUSA300_RS09160 ←	iron‑regulated surface determinant protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,846,875	0	G	A	100.0%	281.4 / NA	86	S10F (TCT→TTT)	SAUSA300_RS09160	iron‑regulated surface determinant protein H
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/85);  total (1/85)

TGCTGCTTGTGCTTGATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAGACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCTATTATTAGTGTTAATTGTAGCGCATCCCAATTAAATATTGCAACC  >  NC_007793/1846781‑1846964                                                                                               |                                                                                          tgctgcTTGTGCTTGATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCtt                                                                                      <  1:1933168/100‑1 (MQ=255)  gctgctTGTGCTTGATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCttt                                                                                     <  1:5282240/100‑2 (MQ=255)  gctgctTGTGCTTGATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTa                                                                                     <  1:2376550/100‑1 (MQ=255)          tgcttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTggg                                                                             <  1:4819053/100‑1 (MQ=255)          tgcttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTggg                                                                             <  1:3404870/100‑1 (MQ=255)          tgcttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTggg                                                                             <  1:5667087/100‑1 (MQ=255)            tgtgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:6052344/98‑1 (MQ=255)            tccgATCTTGAGAGGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:4269546/97‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTTACAATGACCTATGCAACGCCTAGAGTTTATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:4227519/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:234010/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:4398885/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:5488601/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:3808786/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:5195548/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:2841911/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:4539918/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:3071762/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:3457625/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:5175702/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:5073541/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:4500452/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:5550155/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:739943/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:1355151/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:6056650/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:4714515/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:1638662/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:1643128/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:4647355/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:1915055/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:1970426/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:463563/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:2020043/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:5648097/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:2155241/100‑1 (MQ=255)            cttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:5538540/100‑1 (MQ=255)            cttgATATTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:4530908/100‑1 (MQ=255)            ctcgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTCGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTg                                                                           <  1:3292972/97‑1 (MQ=255)             ttgGTGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTgc                                                                          <  1:3714143/100‑2 (MQ=255)             ttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTggggta                                                                          <  1:1067648/100‑4 (MQ=255)             ttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTgg                                                                          <  1:247401/100‑2 (MQ=255)             ttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTgc                                                                          <  1:93795/100‑2 (MQ=255)             ttgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGa                                                                          <  1:2521007/100‑1 (MQ=255)              tgttGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGCCCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGGGAt                                                                         >  1:4979733/4‑100 (MQ=255)              tgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTggg                                                                         <  1:289635/100‑3 (MQ=255)              tgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGag                                                                         <  1:399920/100‑2 (MQ=255)              tgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGag                                                                         <  1:3887569/100‑2 (MQ=255)              tgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGag                                                                         <  1:4281053/100‑2 (MQ=255)              tgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGag                                                                         <  1:3786366/100‑2 (MQ=255)              tgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGag                                                                         <  1:3250097/100‑2 (MQ=255)              tgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGag                                                                         <  1:1599521/100‑2 (MQ=255)              tgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGaa                                                                         <  1:5768568/100‑2 (MQ=255)              tgATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAt                                                                         <  1:378519/100‑1 (MQ=255)              tctgtttGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGag                                                                         <  1:6073720/95‑2 (MQ=255)              atctGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAt                                                                         <  1:1973552/97‑1 (MQ=255)               gATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAtg                                                                        <  1:5132210/100‑1 (MQ=255)               gATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAtg                                                                        <  1:5018043/100‑1 (MQ=255)               gATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAtg                                                                        <  1:4016873/100‑1 (MQ=255)               gATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAtg                                                                        <  1:483403/100‑1 (MQ=255)               gATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAtg                                                                        <  1:4828667/100‑1 (MQ=255)               gATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAta                                                                        <  1:513176/100‑2 (MQ=255)               cttGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGagt                                                                        <  1:1837154/98‑3 (MQ=255)                aTGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAtgt                                                                       <  1:4528761/100‑1 (MQ=255)                 tGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAtgtt                                                                      <  1:6173429/100‑1 (MQ=255)                   ttGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATCTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAtgtttg                                                                    <  1:2255808/100‑1 (MQ=255)                    tGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGGGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAtgtttgg                                                                   <  1:2758201/100‑2 (MQ=255)                    tGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAtgtttgg                                                                   <  1:5399278/100‑2 (MQ=255)                    tGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAtgtttgg                                                                   <  1:754266/100‑2 (MQ=255)                    tGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGAtgtgtgc                                                                   <  1:2392425/100‑2 (MQ=255)                          gTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCAtgt                                                             <  1:2783567/100‑1 (MQ=255)                          gTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCAtgt                                                             <  1:774934/100‑1 (MQ=255)                             aTTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCAtgttgt                                                          <  1:1352098/100‑1 (MQ=255)                              ttAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCATgttgtt                                                         <  1:4841657/100‑1 (MQ=255)                                           aCTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCtatt                                            <  1:209502/100‑1 (MQ=255)                                           aCTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCtatt                                            <  1:3535201/100‑1 (MQ=255)                                            cTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCtatta                                           <  1:5441196/100‑1 (MQ=255)                                            cTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAACAGAAAAACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCtattt                                           <  1:5779393/100‑2 (MQ=255)                                              gACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCTattatt                                         <  1:1427992/100‑1 (MQ=255)                                                gctcttccGATCTAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTAATGTTTGTTCATGTTGTTAACAACTCCTAttattat                                       <  1:1068665/94‑2 (MQ=255)                                                cAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCTattattgg                                       <  1:2218734/100‑3 (MQ=255)                                                cAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCTattattgc                                       <  1:1859325/100‑3 (MQ=255)                                                cAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCTattattgc                                       <  1:4865068/100‑3 (MQ=255)                                                   tGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCTATTATTAgcaa                                    <  1:5745659/100‑4 (MQ=255)                                                              aCGCCTAGAGTTGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCTATTATTAGTGTTAATTGTAgcg                         <  1:2032296/100‑1 (MQ=255)                                                                        ttGATTTTCTAATAGAATAGAAAAACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCTATTATTAGTGTTAATTGTAGCGCATCCCAAtt               <  1:3642648/100‑1 (MQ=255)                                                                                     agaatagaaAAACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCTATTATTAGTGTTAATTGTAGCGCATCCCAATTAAATATTGCAAcc  <  1:1233688/100‑1 (MQ=255)                                                                                               |                                                                                          TGCTGCTTGTGCTTGATGTTGAGAAGTAATTAAAAATAGTGTACTGACAATGACCGATGCAACGCCTAGAGTTGATTTTCTAATAGAATAGAAAGACCTTAATTTTGGGTGATGTTTGTTCATGTTGTTAACAACTCCTATTATTAGTGTTAATTGTAGCGCATCCCAATTAAATATTGCAACC  >  NC_007793/1846781‑1846964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑