Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,269,542	A→T	I18I (ATA→ATT)	polC →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,269,542	0	A	T	100.0%	127.5 / NA	39	I18I (ATA→ATT)	polC	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (38/1);  total (38/1)

GAACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATATCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGAA  >  NC_007793/1269449‑1269623                                                                                              |                                                                                  caaCACGTAAAAGTAACTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATttcaaat                                                                             >  1:4662473/2‑100 (MQ=255)     acGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAAt                                                                         >  1:369316/1‑100 (MQ=255)                      aTTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTa                                                        >  1:1438658/1‑100 (MQ=255)                      aTTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTa                                                        >  1:1481583/1‑100 (MQ=255)                         tAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAAt                                                     >  1:544976/1‑100 (MQ=255)                                        cctGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGaca                                      >  1:463660/3‑100 (MQ=255)                                        cTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGaca                                      >  1:4112669/1‑100 (MQ=255)                                         ttGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGacac                                     >  1:5119745/1‑100 (MQ=255)                                         ctGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGacac                                     >  1:5979518/2‑100 (MQ=255)                                         ctGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGacac                                     >  1:5145543/2‑100 (MQ=255)                                         atGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGacac                                     >  1:1737943/2‑100 (MQ=255)                                          tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGGAATTTTAAATTCAGGTGAACTGACACg                                    >  1:1810580/1‑100 (MQ=255)                                          tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:5234390/1‑100 (MQ=255)                                          tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:5435143/1‑100 (MQ=255)                                          tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:5839784/1‑100 (MQ=255)                                          tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:3888340/1‑100 (MQ=255)                                          tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:1124891/1‑100 (MQ=255)                                          tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:1120798/1‑100 (MQ=255)                                          tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:1400308/1‑100 (MQ=255)                                          tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:1510024/1‑100 (MQ=255)                                           ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:3444849/1‑100 (MQ=255)                                           ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:2462497/1‑100 (MQ=255)                                           ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:3564834/1‑100 (MQ=255)                                           ggCAATGACAGAGCAACAAAAATTTAAAGCGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:4473005/1‑100 (MQ=255)                                             cAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGtat                                 <  1:3742410/100‑1 (MQ=255)                                              gaTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGtata                                >  1:2927390/2‑100 (MQ=255)                                               aTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAg                               >  1:3045752/1‑100 (MQ=255)                                               aTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAg                               >  1:1575486/1‑100 (MQ=255)                                               aTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAg                               >  1:2319894/1‑100 (MQ=255)                                                    agggCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTACAGAtcgg                          >  1:3315790/4‑97 (MQ=255)                                                      ggCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTc                        >  1:5822920/2‑100 (MQ=255)                                                      agCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTc                        >  1:946605/1‑100 (MQ=255)                                                         gacaaAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTaa                     >  1:2434744/2‑100 (MQ=255)                                                          acaaAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAAc                    >  1:170607/1‑100 (MQ=255)                                                          acaaAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAAc                    >  1:4993325/1‑100 (MQ=255)                                                                       tagcttgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACAt       >  1:4767457/3‑100 (MQ=255)                                                                         gcttgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATgg     >  1:3300954/1‑100 (MQ=255)                                                                           ttgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGa   >  1:2596993/1‑100 (MQ=255)                                                                            tgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGaa  >  1:4406847/1‑100 (MQ=255)                                                                            tgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGaa  >  1:3746654/1‑100 (MQ=255)                                                                                              |                                                                                  GAACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATATCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGAA  >  NC_007793/1269449‑1269623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑