| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 1,122,225 | G→A | intergenic (+28/+126) | rpmF → / ← SAUSA300_RS05535 | 50S ribosomal protein L32/iron‑regulated surface determinant protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 1,122,225 | 0 | G | A | 80.0% | 19.0 / 0.1 | 15 | intergenic (+28/+126) | rpmF/SAUSA300_RS05535 | 50S ribosomal protein L32/iron‑regulated surface determinant protein B |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (0/12); total (1/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
GAATACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCGCTTAAAATGATTGTTTTAAGTGGGACTTTTTATATTGCGAAAAATAATTGGCGAACGAGGTAACTGGAT > NC_007793/1122126‑1122294 | gAATACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCg < 1:1686358/100‑1 (MQ=255)gAATACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCg < 1:237357/100‑1 (MQ=255) aTACAAATTATCACACCGTGTATGT‑AAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACtt < 1:3574389/100‑1 (MQ=255) tACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACtt < 1:815728/100‑1 (MQ=255) tACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACtt < 1:5327262/100‑1 (MQ=255) tACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACtg < 1:1405680/100‑2 (MQ=255) ttATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACtcgtcata < 1:5398099/100‑8 (MQ=255) cacaCCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCGCTTAAAATGATTg > 1:4833897/1‑100 (MQ=255) acacCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACTTaaaacaggtgg < 1:470808/100‑8 (MQ=255) cacCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACTTaaaacaggtttg < 1:3391723/100‑9 (MQ=39) acCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACTTaaaacagcagtaa < 1:402281/100‑10 (MQ=39) tCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACTTAAAACAATCATTTTAAGCGGGACTTTTTATATTgac < 1:124105/100‑3 (MQ=17) cTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACTTAAAACAATCATTTTAAGCGGGACTTTTTatataaagt < 1:4845306/100‑6 (MQ=17) aCAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACTTAAAACAATCATTTTAAGCGGGACTTTTTATATTgggtccc < 1:2435404/100‑7 (MQ=17) aCAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCACTTAAAACAATCATTTTAAGCGGGACTTTTTATATTGCggggc < 1:6008003/100‑5 (MQ=17) agaagTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCGCTTAAAATGATTGTTTTAAGTGGGACTTTTTATATTGCGAAAAATAATTGGCg < 1:4950035/100‑1 (MQ=255) taatTTTAGTTACTCAATATAAAAAGTCCCGCTTAAAATGATTGTTTTAAGTGGGACTTTTTATATTGCGAAAAATAATTGGCGAACGAGGTAACTGGAt < 1:3039796/100‑1 (MQ=255) | GAATACAAATTATCACACCGTGTATGTAAAAACTGTGGTTCTTACAATGGCGAAGAAGTAGCAGCTAAATAATTTTAGTTACTCAATATAAAAAGTCCCGCTTAAAATGATTGTTTTAAGTGGGACTTTTTATATTGCGAAAAATAATTGGCGAACGAGGTAACTGGAT > NC_007793/1122126‑1122294 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |