Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	903,334	T→C	intergenic (+41/‑149)	SAUSA300_RS04430 → / → SAUSA300_RS04435	Fe‑S cluster assembly protein SufB/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	903,334	0	T	C	100.0%	39.8 / NA	17	intergenic (+41/‑149)	SAUSA300_RS04430/SAUSA300_RS04435	Fe‑S cluster assembly protein SufB/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/0);  total (17/0)

TACGCGGTCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAACAAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTA  >  NC_007793/903237‑903433                                                                                                  |                                                                                                    tACGCGGTCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACgg                                                                                                   >  1:1439434/1‑100 (MQ=255) tACGCGGTCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACgg                                                                                                   >  1:5041639/1‑100 (MQ=255) tACGCGGTCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATCCTTAACTGTCGAAAACgg                                                                                                   >  1:4588079/1‑100 (MQ=255)     cgGTCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTAt                                                                                               >  1:4975881/1‑100 (MQ=255)               gAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCAtt                                                                                     >  1:4082568/1‑100 (MQ=255)               agaCCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCAtt                                                                                     >  1:3174432/3‑100 (MQ=255)                 tgggTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCAtttt                                                                                   >  1:3556371/4‑100 (MQ=255)                       tAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCATTTTGATAcc                                                                             >  1:1218264/1‑99 (MQ=255)                         tgcAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCATTTTGATAccat                                                                           >  1:1443175/3‑97 (MQ=255)                         aTCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCATTTTGATAccat                                                                           >  1:1724857/1‑97 (MQ=255)                          acAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCATTTTGATAccatt                                                                          >  1:399621/2‑96 (MQ=255)                           cAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCATTTTGATAccattt                                                                         >  1:5878064/1‑95 (MQ=255)                           cAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCATTTTGATAccattt                                                                         >  1:38147/1‑95 (MQ=255)                           cAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCATTTTGATAccattt                                                                         >  1:3761859/1‑95 (MQ=255)                            caGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCATTTTGATAccattta                                                                        >  1:1486312/2‑94 (MQ=255)                             aGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCATTTTGATAccatttac                                                                       >  1:1585063/1‑93 (MQ=255)                             aGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCTATTCTTAACTGTCGAAAACGGTTATTGATACCATTTTGATAccatttac                                                                       >  1:5115941/1‑93 (MQ=255)                                                                                                  tGGCTATTGATACCATTTTGATACTGAATATAACAAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTa  <  1:1968248/100‑1 (MQ=255)                                                                                                  |                                                                                                    TACGCGGTCGAAATGAACCGTTTAATCAAGTTCGAAATGGAAGGTAGTATTGGATAGCTTTAAACCGCGTTGTTAAGCCATTCTTGACTTCCGAAAATGGCTATTGATACCATTTTGATACTGAATATAACAAAAAGCCACATTACTGTGGCTTTTTTTGTTTTATAACTAAATCGGATTGATAGATAAGCTTTGTA  >  NC_007793/903237‑903433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑