Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	658,465	T→A	intergenic (+30/‑266)	SAUSA300_RS03130 → / → SAUSA300_RS03135	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	658,465	0	T	A	100.0%	20.3 / NA	8	intergenic (+30/‑266)	SAUSA300_RS03130/SAUSA300_RS03135	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (3/5);  total (3/5)

TTATGAATATGCATTCAATTATAGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTAATTTAGGGGTGGTAAACAGTGCTTTGTGAATCTAGACAAATTTATAAAAATCCTAAATATCGAGTTATTAGATATAATAATGA  >  NC_007793/658374‑658546                                                                                            |                                                                                  ttatgAATATGCATTCAATTATAGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAGGggt                                                                           <  1:1834289/100‑1 (MQ=255) ttatgAATATGCATTCAATTATAGAT‑AAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAggggca                                                                          <  1:168881/100‑3 (MQ=255)    tgAATATGCATTCAATTATAGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAGGggtggt                                                                        <  1:354686/100‑1 (MQ=255)     gAATATGCATTCAATTATAGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAGGGGTGGTa                                                                       <  1:4459849/100‑1 (MQ=255)         atGCATTCAATTATAGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAGGGGTGGTAAACa                                                                   >  1:4512039/1‑100 (MQ=255)                     ataGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAGGGGTGGTAAACAGTGCTTTGTGaa                                                       >  1:6227822/1‑100 (MQ=255)                      taGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTTAATTAGGGGTGGTAAACAGTGCTTTGTGaag                                                      <  1:226439/100‑2 (MQ=255)                                                                          gttagttTTCAATTGTTAATTAGGGGTGGTAAACAGTGCTTTGTGAATCTAGACAAATTTATAAAAATCCTAAATATCGAGTTATTAGATATAATAATGa  >  1:6148773/1‑100 (MQ=255)                                                                                            |                                                                                  TTATGAATATGCATTCAATTATAGATAAAAAAGTACATATATTCTTAAAGTCTAATAAATAGTTACAAATTTAGTTAGTTTTCAATTGTAATTTAGGGGTGGTAAACAGTGCTTTGTGAATCTAGACAAATTTATAAAAATCCTAAATATCGAGTTATTAGATATAATAATGA  >  NC_007793/658374‑658546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑