Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	556,082	T→C	intergenic (+443/‑115)	SAUSA300_RS02660 → / → SAUSA300_RS02665	lysine‑‑tRNA ligase/5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	556,082	0	T	C	100.0%	53.4 / NA	22	intergenic (+443/‑115)	SAUSA300_RS02660/SAUSA300_RS02665	lysine‑‑tRNA ligase/5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/3);  total (19/3)

GAATTAGAGGGCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGTCAAAAACAGACGAAACACACTAAAAGCTGATGTGACAAAGTTTACATCAAAGTGTAAAATTAACTATTGCACCTTATTAA  >  NC_007793/555986‑556162                                                                                                 |                                                                                 gAATTAGAGGGCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCat                                                                               >  1:4797935/1‑99 (MQ=255)      agagGGCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAATAACa                                                                          >  1:518782/1‑100 (MQ=255)      agagGGCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAAACa                                                                          >  1:2804420/1‑100 (MQ=255)      agagGGCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAAACa                                                                          >  1:4087992/1‑100 (MQ=255)      acagGGCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCaaaacag                                                                          >  1:4872257/3‑97 (MQ=255)       gggggCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGGTTAAAAGTGTTGACTTTATCAACTGAATGAAGTAATATATAAAAGTCGCCAACAACAg                                                                         >  1:5958909/3‑100 (MQ=255)       acgggCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAAACAg                                                                         >  1:6208801/3‑100 (MQ=255)        agGGCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAGACAGa                                                                        >  1:2659338/1‑100 (MQ=255)         ttgCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAAACAGat                                                                       >  1:1857648/3‑99 (MQ=255)         gggCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCatacagatcg                                                                       >  1:1718760/1‑91 (MQ=255)          tgCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCATAAACAGatc                                                                      >  1:3727340/2‑98 (MQ=255)          ggCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAGGTAATATATAAAAGTCGCCgaaacagatcg                                                                      >  1:975284/1‑93 (MQ=255)           gCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAGTATATAAAAGTCGCCAAAATCAGatcg                                                                     >  1:5649130/1‑97 (MQ=255)           gCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCaatacagatcgg                                                                     >  1:5707143/1‑90 (MQ=255)           acccGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCATAAACAGatcg                                                                     >  1:480867/4‑97 (MQ=255)            taCGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCagaacagatcgga                                                                    >  1:2075657/2‑91 (MQ=255)             aCGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCGAAAACAGatcgga                                                                   >  1:3809665/1‑95 (MQ=255)              ccagaAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAGAAACAGatcggaa                                                                  >  1:2238863/3‑94 (MQ=255)                            tttGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAAACAGACGAAACACACTAAAAGCTGa                                                    <  1:651175/100‑1 (MQ=255)                             ttGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAAACAGACGAAACACACTAAAAGCTGaa                                                   <  1:5904209/100‑2 (MQ=255)                                                               tttATCAATTGAATGAAGTAATATATAAAAGTCGCCAAAAACAGACGAAACACACTAAAAGCTGATGTGACAAAGTTTACATCAAAGTGTAAAATTAACt                 <  1:715743/100‑1 (MQ=255)                                                                              aaGTAATATATAAAAGTCGCCAAAAACAGACGAAACACACTAAAAGCTGATGTGACAAAGTTTACATCAAAGTGTAAAATTAACTATTGCACCTTattaa  >  1:4220047/1‑100 (MQ=255)                                                                                                 |                                                                                 GAATTAGAGGGCACGAGAAATGTCAGTTTTGAAGAATAAAAAAGTTGATTAAAAGTGTTGACTTTATCAATTGAATGAAGTAATATATAAAAGTCGTCAAAAACAGACGAAACACACTAAAAGCTGATGTGACAAAGTTTACATCAAAGTGTAAAATTAACTATTGCACCTTATTAA  >  NC_007793/555986‑556162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑