Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	376,231	T→G	S200R (AGT→CGT)	SAUSA300_RS01715 ←	NADH‑dependent flavin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	376,231	0	T	G	100.0%	54.2 / NA	21	S200R (AGT→CGT)	SAUSA300_RS01715	NADH‑dependent flavin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/11);  total (10/11)

CTGGCGTTGCTCGAAATCCTAAAATAAAATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACTATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAATT  >  NC_007793/376135‑376325                                                                                                 |                                                                                               ctGGCGTTGCTCGAAATCCTAAAATAAAATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGAtt                                                                                             >  1:4162857/1‑100 (MQ=255)   ggCGTTGCTCGAAATCCTAAAATAAAATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGAtttt                                                                                           >  1:5381082/1‑100 (MQ=255)       ttGCTCGAAATCCTAAAATAAAATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTaa                                                                                       >  1:4560409/1‑100 (MQ=255)                            aatTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTc                                                                  <  1:2246904/100‑1 (MQ=255)                             atTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCt                                                                 <  1:434038/100‑1 (MQ=255)                               tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                               <  1:3680555/100‑1 (MQ=255)                               tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                               <  1:802683/100‑1 (MQ=255)                               tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                               <  1:45478/100‑1 (MQ=255)                                                   atcaCTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTg                                           <  1:2751557/100‑1 (MQ=255)                                                   atcaCTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTg                                           <  1:5482261/100‑1 (MQ=255)                                                            tGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATg                                  >  1:5012084/1‑100 (MQ=255)                                                                    acgcaTAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATc                          <  1:2145135/100‑1 (MQ=255)                                                                         tAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCaa                     <  1:995645/100‑1 (MQ=255)                                                                                 aaaCATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTtgtg             <  1:5330944/100‑1 (MQ=255)                                                                                 aaaCATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTtgtg             <  1:3769798/100‑1 (MQ=255)                                                                                            tGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAAtt  >  1:5874580/1‑100 (MQ=255)                                                                                            tGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAAtt  >  1:6150986/1‑100 (MQ=255)                                                                                            tGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAAtt  >  1:2344393/1‑100 (MQ=255)                                                                                            tGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAAtt  >  1:923717/1‑100 (MQ=255)                                                                                            tGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAAtt  >  1:972637/1‑100 (MQ=255)                                                                                            tGCACGATTTTTTAATGAGTCGGCACCGTAATGGACTGTACGTTTATTCGAAAAAGGTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAAtt  >  1:2282783/1‑100 (MQ=255)                                                                                                 |                                                                                               CTGGCGTTGCTCGAAATCCTAAAATAAAATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACTATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAATT  >  NC_007793/376135‑376325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑