Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	503,675	0	A	T	72.2%	25.1 / 9.1	20	noncoding (25/93 nt)	SAUSA300_RS02390	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base T (13/0);  total (20/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGTTTAGGAGGAATACCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATAT  >  NC_007793/503643‑503768                                 |                                                                                              agtgtTTAGGAGGAATACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGCATCCCTCTTCCTCCGcc                            >  1:3154321/2‑100 (MQ=255)      catggaggaATACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCaa                       >  1:5374655/4‑100 (MQ=255)       agcaaggaATACCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAat                      >  1:2167218/5‑100 (MQ=255)              taTACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTtata               >  1:4733326/2‑100 (MQ=255)               aTACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTtattt              >  1:1895027/1‑98 (MQ=255)                 aCCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTa            >  1:5448607/1‑100 (MQ=255)                 aCCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTa            >  1:2979825/1‑100 (MQ=255)                 aCCCAAGTCCGGCTGACGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTa            >  1:4724098/1‑100 (MQ=255)                 aCCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGAAACCCTCTTCCTCCGCCATCAATATTtaaagaa            >  1:2641220/1‑95 (MQ=14)                    cAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCTCTCTTCCTCCGCCATCAATATTtaagggcggg         >  1:4248953/1‑92 (MQ=14)                      ggTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTc       >  1:6091012/2‑100 (MQ=255)                      aGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTtattaaaagaag       >  1:87229/1‑91 (MQ=255)                       tcccGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCt      >  1:4794045/3‑100 (MQ=255)                       atCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCt      >  1:4145861/2‑100 (MQ=255)                        cccGGCTGAGGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAAATCta     >  1:3354288/2‑100 (MQ=255)                        cccGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCta     >  1:3863304/2‑100 (MQ=255)                         ccGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGGGGGGGGTCGAATCCCTCTTTCTCCGCCATCAATAATTATATTAAATTCtat    >  1:1995744/1‑100 (MQ=18)                           ggCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCtatat  >  1:2463666/1‑100 (MQ=255)                           ggCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCtatat  >  1:246233/1‑100 (MQ=255)                           ggCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAAATCtatat  >  1:1470241/1‑100 (MQ=255)                                 |                                                                                              TGTGTTTAGGAGGAATACCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATAT  >  NC_007793/503643‑503768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑