Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1247,6860AC38.8% 87.7 / 21.6 67intergenic (+111567/+15467)unknown/unknowntRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (28/13);  new base C (1/25);  total (29/38)
Fisher's exact test for biased strand distribution p-value = 1.03e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCTGCCAGCCCGTGCTGATTTGGCTATTGATTTGCTGCCTGATTTCCTCCATTATTTCACGCCAATTT‑AATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCC‑ATCGGATTCAAGAAGAAATGCAACAAGACATGAAGGACAACTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGTCCGATCGCGTGCAAAACGGCATGTTCGGCGCC  >  1/247438‑247930
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      
gCTGCCAGCCCGTGCTGATTTGGCTATTGATTTGCTGCCTGATTTCCTCCATTATTTCACGCCAATTT‑AATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCC‑ATCGGATTCAAGAAGAAATGCAACAAGACATGAAGGACAACTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCAc                                                                                                                                                                                                                                                       <  1:124068/250‑1 (MQ=255)
 cTGCCAGCCCGTGCTGATTTGGCTATTGATTTGCTGCCTGATTTCCTCCATTATTTCACGCCAATTT‑AATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCCCC‑CTCGGGTTCAAGAAGAAAAGCAACAAAAAATGAAGGACAAATCGTAAAAAAGCCTCAAAA‑ACCACCCCCC‑TTTTTTTCCGGACCCTTCGCCGGGGCACCCCTGTTTGGCTGGACGACGCGCCCTTGCCGTTA‑TTTTCAcc                                                                                                                                                                                                                                                      >  1:456660/1‑250 (MQ=255)
  tGCCAGCCCGTGCTGATTTGGCTATTGATTTGCTGCCTGATTTCCTCCATTATTTCACGCCAATTT‑AATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCC‑ATCGGATTCAAGAAGAAATGCAACAAGACATGAAGGACAACTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGc                                                                                                                                                                                                                                                    <  1:755724/251‑1 (MQ=255)
                             ttttGCTGCCTGTTTCCCCCCATTTTTCCCCGCAAATTT‑AACCCGAGGCGTTTCTATCATTTTGCGGCGGGGCGCTTCCGCC‑ATGGGATTCAAGAAAAAATGCAACAAGACATGAAGGACAACTCGAAACCATGCCTAAAAA‑CCCCCCGCCT‑TTTTAGCGCGGACGTTCCCCCGTGGCCCCCATGTTGGCCGGGACGCCGCGCCATTGCCGTTA‑TTTCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTac                                                                                                                                                                                                                        <  1:840730/250‑1 (MQ=255)
                               ttGCTGCCTTTTTTCCCCCATTATTCCCCCCAAATTT‑AACCCGAAGCCTTTCTATATTTTTGCGGCGGGGCGCTCCCGCC‑ATCGGATCAAAAAAAAAAGGCAAAAAGACAGGAGGGAAACCTCGAAAACATCCCTCAAAC‑CCCCCCGCTC‑TTTTACCGCGGACGCTTCGCCGGGCCCCCCATGTTGGACTGGCCGACGCGCCATTGCCTTTA‑TTCCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTaca‑c                                                                                                                                                                                                                     <  1:460755/251‑1 (MQ=255)
                               ttGCTGCCTGTTTTCCTCCATTATTCCCCGCCAATTT‑AACCCGAAGCCGTTCTATCATTTTGGGGCGGGGCGCTCCCGCC‑ATCGGATAAAAGAAAAAATGCAAAAAGACATGAAGGACAACTCGAAAACATGCCTAAAAC‑CCCCCCCCAC‑TTTTATCGCGGACGCTTCGCCGTGCCCCCCATGTTGGACTGGACGCCGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTaca‑c                                                                                                                                                                                                                     <  1:457850/251‑1 (MQ=255)
                               ttGCTGCCTGTTTTCCCCCATTATTTCACGCCAATTT‑AATCCGAAGCCTTTCTATCATTTGGCGGGGGGGCGCTTCCCCC‑ATGGGATTAAAAAAGAAATGCAAAAAGACATGAAGGACAACTCGAAAACATGCCTCAAAC‑CCCCCCGCTC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCTTGTTGGACTGGACGCCGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTaca‑c                                                                                                                                                                                                                     <  1:594235/251‑1 (MQ=255)
                                       tgtttccctccATTTTTTCACCCCAATTT‑AATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGTTTCCGCC‑ATCGGATTAAAGAAGAAATGCAACAAGACATAAAGGACAACTCGAAAACATGCCTCAAAC‑CCCCCCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTCCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAAtgg                                                                                                                                                                                                             <  1:753357/245‑1 (MQ=255)
                                         tttccctccATTA‑TTCCCCCCAATTTAAACCCGAGGCCGTTCTATCATTTTGCGGCGGGGCGCTCCCGCC‑ATCGAATTAAAAAAAAAAGGAAAAAAAACATGAAGGACAACCCGAAAACATGCCTAAAAC‑CCCCCCGCCC‑TTTTACCGCGGACGCTTCGCCGTGGCACCCATGTGGGACGGGACGACGCGCCATTGCCGTTA‑TTTCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAAtggtg                                                                                                                                                                                                           <  1:749968/247‑1 (MQ=255)
                                              ctccTTTATTTCACGCAAATTA‑AATCCAAGGCCGTTTTATAATTTGGGGGCGGGGCCCTTCCGCC‑ATCGGATAAAAGAAGAAATGCAACAAGACATGAAGGACACCTCGTAAACATGCCTCAAAC‑CCCCCCGCAT‑TTTTATCGCGGACGCTTCGCCGTGGCCCCCATGTTGGACGGGACGCCGCGCCATTGCCTTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGac                                                                                                                                                                                                      <  1:461023/251‑1 (MQ=255)
                                              ctccATTATTCCCCG‑CAATTTAAACCCGAAGCCGTTCTATCATTTGGGGGGGGGGCGCTTCCGCC‑ATGGGATTCAAAAAGAAATGAAAAAAGACATGAAGAACAACTCGTAAACATGCCTCAAAC‑CCCCCCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCCCCCATGTTGGACGGGACGACGCGCAATTGCCGTTA‑TTCCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGac                                                                                                                                                                                                      <  1:763101/251‑1 (MQ=255)
                                                     ttttCACGCCAATTT‑ACCCCGAAGCCTTTCTATCATTTTGCGGCGGGGCGCTTCCGCA‑ATCGAATTCAAAAAGAAATGCAAAAAGACAGGAAGGACACCTCGAAAACATGCCTAAAAA‑CCCACCGCCC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGGCGCGCCATTGCCGTTT‑TTTCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGcc                                                                                                                                                                                               <  1:213107/250‑1 (MQ=255)
                                                      tttccccgcaaattaaacccGAAGCCTTTCTATCTTTTTGCGGGGGGGCGCTCCCGCC‑ATCGGTTTAAAAAAAAAATGAAAAAAGACATGAAGAACAACTCGAAAACATGCCTCAAAC‑CCCCCCGCAT‑TTTTATCGCGGACGCTTCGCCGTGGCCCCCATGTTGGACTGGACGACGCGCCATTGCCGTTT‑TTTCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGcc                                                                                                                                                                                               <  1:18047/233‑1 (MQ=255)
                                                       tccccGCCAATTA‑AACCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCC‑ATCGGATTAAAGAAAAAATGCAAAAAAACATGAAGGAAAACTCGAAAACATCCCTAAAAC‑CCCCCCGCCC‑TTTTATCGCGGCCGCTTCGCCGGGGCCCCCATGTTGGACGGGACGACGCCCCATTGCCTTTA‑TTTCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCcat                                                                                                                                                                                             <  1:619437/247‑1 (MQ=255)
                                                            cccaaattaaacccGAAGCCGTTCTATCATTTGGCGGCGGGGCGCTTCCCCC‑ATCGAATAAAAAAAAAAAGGCAAAAAGAAATGAAGGACACCTCGAAACCATCCCTCAAAC‑CCCCCCGCAT‑TTTTACCGCGGCCGCTTCGCCGGGGCCCCCATGTTGGACTGGACGCCGCGCCATTGCCGTTA‑TTCCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCatcatc                                                                                                                                                                                         <  1:84211/239‑1 (MQ=255)
                                                            ccaaaTTT‑AACCCGAAGCCGTTCTATCATTTTGCGGGGGGGCGCTTCCGCC‑ATCGGATTAAAGAAAAAATGCAACAAGACATGAAGAACACCTCGTAAACATGCCTCAAAC‑CCCACCGCCC‑TTTTATCGCGGACGCTTCGCCGGGGCCCCCATGTTGGACTGGACGACGCGCCATTGCCGTTT‑TTTCCACCGCA‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATcc                                                                                                                                                                                        <  1:346568/248‑1 (MQ=255)
                                                              aaaTTA‑AATCCGAAGCCTTTCTATCATTTTGCGGCGGGGCGCTCCCGCC‑ATCGGATCAAAGAAAAAATGAAACAAGACATGAGGGACAACTCGTAAACATGCCTCAAAC‑CCCCCCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCCCCCATGTTGGACTGGACGACGCGCAATTGCCGTTA‑TTTCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCAc                                                                                                                                                                                      <  1:105603/250‑1 (MQ=255)
                                                               atttt‑AACCCGAAGCCGTTCTATCATTTGGGGGGGGGGCGCTTCCGCC‑GTCGGATCCAAGAAGAAATGAAACAAGACATAAAGGACAACGCGTAAACATGCCTCAAAC‑CCCCCCGCAC‑TTTTATCGCGGACGTTTCGCCGTGGCCCCAATTTGGGACGGGCCGGCGCGCCATTGCCGTTA‑TTCCCACCGCT‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAAtgg                                                                                                                                                                                                             <  1:585382/225‑1 (MQ=255)
                                                               aaTTT‑AATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCC‑AACGGATTCAAAAAAAAAAGCAACAAGACATGAAGGACAACACGTAAAAATGCCTCAAAA‑ACCACCCCCC‑TTTTATTGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTTCCGTTA‑TTTTCACCGCC‑AAATTTCCCCCCATGCCTTGCTTTACACCGGAAA‑GGGGGGGGCAAAAGACATCATCCAc                                                                                                                                                                                      >  1:107060/1‑250 (MQ=255)
                                                                aTTT‑AATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCC‑ATCGGATTCAAGAAGAAAAGCAACAAAACATGAAGGACAACTCGTAAACATGCCTCAAAA‑ACCACCCCCC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCCTGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTTCACCCCC‑CATTTTCCCGCCATGCCTTGCTTTTCA‑CGGAAATGGTGGCGACACaaacc                                                                                                                                                                                               >  1:170443/1‑237 (MQ=255)
                                                                 ttt‑AATCCGAAGCCTTTCTATCATTTTGCGGCGGGGCGCTTCCCCC‑ATCGGATTCAAAAAGAAATGCAACAAGACATGAAGGACACCTCGTAAACATCCCTCAAAC‑CCCCCCGCCC‑TTTTATCGCGGACGCTTCGCCGTGGCCCCCATGTTGGACGGGCCGCCGCGCCTTTGCCGTTA‑TTTCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGcc                                                                                                                                                                                   <  1:623954/251‑1 (MQ=255)
                                                                 ttt‑AACCCGAAGCGTTTCTATCATTTGGCGGCGGGGCGTTCCCGCC‑ATGGGATACAAAAAAAAATGCAACAAGACAGAAAGGACAACTCGAAAACATGCCTCAAAC‑CCCACCGCAC‑TTTTACCGCGGACGCTCCGCCGTGGCCCCCATGTTGGACGGGACGACGCGCCATTGCGTTTT‑TTTCCCCCGCC‑AATTTCCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGcc                                                                                                                                                                                   <  1:3008/251‑1 (MQ=255)
                                                                      acccGAAGCCGTTCTATCTTTTTGCGGCGGGGCCCTTCCGCC‑ATCGGATTAAAAAAGAAATCCACCAAGAAATGAAGAACACCTCTTAAACATCCCTCAAAC‑CCCCCCGCCC‑TTTTACCGCGGCCGCTTCGCCGTGCCCCCCATTTTGGACTGGACGACGCGCCTTTGCCGTTA‑TTTCCCCCCCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGcc                                                                                                                                                                                               <  1:361614/233‑1 (MQ=255)
                                                                      aTCCGAAGCCGTTCTATCATTTTGCGGCGGGGGGCCTCCCCC‑CTCGGGTTCAAGAAGAAAAGCAACAAAAAAAGAAGGACAAATCGTAAACAAGCCCCAAAA‑CCCACCGCAC‑CTTTTTTGCGGGCGCTTCGCCCTGGCACCCCTGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTTCACCCCC‑CATTTTTCCCCCCCGCCCTTCTTTTCA‑CGGAAAAGGTGGGGACACAAACCATCATCCACGcccaaa                                                                                                                                                                               >  1:403479/1‑247 (MQ=255)
                                                                       cccgagccctttCTATCATTTTGGGGCGGGGCGCTCCCGCC‑ATCGAATTCAAAAAAAAAGGCACCAAGAAATGAGGGACACCCCGAAACCATGCCAAAAAC‑CCCCCCGCTC‑TTTTACCGCGGACGCTCCGCCGTGGCCCCCATGTGGGACGGGACGCCGCGCATTTGCGGTTT‑TTTCCACCGCA‑ATTTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAata                                                                                                                                                                              <  1:220160/241‑1 (MQ=255)
                                                                       cccaaaGCCGTCTTATCTTTTGGCGGCGGGGCGCTCCCGCC‑ATGGGATTAAAAAAAAAATCAAAAAAGACATGAAGGACAACTCGAAAACATCCCTAAAAC‑CCCCCCGCAC‑TTTTACCGCGGACGCTTCCCGGTGGCCCCCATTTTGGCCTGGACGCCGCCCCATTGCGTTTT‑TTTCCACCGCC‑ATTTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAata                                                                                                                                                                              <  1:349915/247‑1 (MQ=255)
                                                                       cccGAGGCCGTTCTTTCATTTGGCGGCGGGGCGCTTCCGCC‑TGGGGATCCAAAAAAAAATGAAACAAGACTTGAAGGACACCTCGAAATCATGCCTCAAAC‑CCCCCCGCAC‑TTTTATCGCGACCGCTTCGCCGGGGCCCCCATTTGGGACTGGCCGACGCGCCATTGCGGTTA‑TTTCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAata                                                                                                                                                                              <  1:782716/250‑1 (MQ=255)
                                                                           aagccgttttattttttggggggggggCGCTTCCCCC‑ATCGAATTCAAAAAAAAATGCAAAAAGACAGGAAGGAAAACCCGTAAACAGGCCCCAAAA‑CCCCCCGCCC‑TTTTTCCGGGGCCGCTTCGCCGTGGCCCCCATGTTGGACTGGACGACGCCCCATTGCCTTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGat                                                                                                                                                                          <  1:679881/228‑1 (MQ=255)
                                                                           aaGCCTTTCTATCATTTGGCGGCGGGGCCCTCCCCCC‑CTCGAATTCAAAAAAAAATCCAACAAGAAATGAAGAACACCTCTTAACCATCCCTAAAAC‑CCCCCCGCCC‑TTTTACCGCGGCCCCTCCCCCGTGGCCCCCATGTTGGCCTGGACGCCGCCCCTTTGCCTTTA‑TTCCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGat                                                                                                                                                                          <  1:180700/251‑1 (MQ=255)
                                                                           aaGCCGTTCTATCTTTTGGCGGCGGGGCCCTTCCCCC‑ATCGAATCAAAGAAAAAATCAAACAAGACAGGAAGGACACCTCGTAAACATGCCTAAAAC‑CCCCCCGCCC‑TTTTACCGCGGCTGCTTCGCCGGGCCCCCCATGTTGGACGGGACGCCGCGCCATTGCCGTTA‑TTCCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGat                                                                                                                                                                          <  1:452882/251‑1 (MQ=255)
                                                                           aaGCCGTTCTATCATTTTGCGGCGGGGCTCTCCCGCC‑TTCGAATTAAAGAAGAAATGCAAAAAGACATGAAGGACAACTCGTAAACATGCCTAAAAC‑CCCCCCGCAC‑TTTTACCGCGGCCGCTTCGCCGTGGCCCCCATTTTGGACTGGACGCCGCGCCTTTGCCTTTA‑TTCCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGat                                                                                                                                                                          <  1:99757/251‑1 (MQ=255)
                                                                           aaGCCGTTCTATATTTTTGCGGCGGGGCG‑TTCCCCCAAGCGAATAAAAAAAAAAAGGCAAAAAAACATGAAGGACACCTCGTAAACATGCCTCAAAC‑CCCCCCGCAC‑TTTTATCGCGGCCGCTTCGCCGTGGCCCCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTCCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGat                                                                                                                                                                          <  1:419851/251‑1 (MQ=255)
                                                                             gCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCC‑CTCCGGTTTAAGAAGAAAAGCAACAAGACAAGAAGGACAACCCGTAAAAAATCCTCAAAA‑ACCACCCCAC‑TTTTTTCGCGGACGCGTCGCCGCGGCACCCCCGTTGGGCTGGACGACGCGCCCTTGCCGTTA‑TTTTCACCCCCAAATTTTCCC‑CCATGCCTTGCTTTTTA‑CGGAAATGGGGGGGACACAAACCCTCATCCCCCCCCAAAATGatca                                                                                                                                                                        >  1:550383/1‑251 (MQ=255)
                                                                                tttCTATCATTTGGCGGGGGGGCGCTTCCCCC‑ATCGGATTAAAAAAAAAATGCAACAAGACATGAAGGACAACTCGAAAACATGCCTCAAAC‑CCCCCCGCTT‑TTTTATCGCGGACGCTTCGCCGTGGCCCCCATGTTGGACTGGACGCCGCGCCATTGCCGTTA‑TTCCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGcc                                                                                                                                                                                               <  1:608881/224‑1 (MQ=255)
                                                                                 ttCTATCATTTTGCGGCGGGGGGCTTCCGCC‑ATCGGGTTCAAGAAGAAAAGCAACAAGACATGAAGGACAACTCGTAAAAAAGCCTCAAAA‑ACCACCGCAC‑CTTTATCGCGGACGCTTCGCCGTGGCACCCACGTTGGACTGGACGACGCGCCCTTGCCGTTATTTTCCCCCCCC‑ATTTTTCCC‑CCCTGCCTTGc                                                                                                                                                                                                                             >  1:118317/1‑195 (MQ=255)
                                                                                 ttCTATCATTTTGCGGCGGGGCGCTTCCGCC‑ATCGGATTCAAGAAGAAATGCAACAAAACATGAAGGACAAATCGTAAACATGCCTCAAAA‑ACCACCGCAC‑TTTTTTCGCGGACGCTTCGCCGTGGGACCCCTGTTTGACTGGACGACGCGCCCTTGCCGTTA‑TTTTCACCCCC‑CAtttttcccccctgcc                                                                                                                                                                                                                                 >  1:846157/1‑180 (MQ=255)
                                                                                           ttGCGGGGGGGCGCTCCCGCC‑ATGGAATTAAAAAAAAAATGCAACAAGAAATAAAGGACAACTCAAAAACATGCCTAAAAC‑CCCCCCGCTT‑TTTTATCGCGGCCGCTCCGCGGTGGCCCCCATGTTGGACGGGACGCCGCGCCTTTGCCGTTA‑TTTCCCCCGCA‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGAcc                                                                                                                                                          <  1:462423/251‑1 (MQ=255)
                                                                                                        ctcccGCC‑ATGGGATTAAAGAAAAAATGCAACAAGACATGAAGGACAACTCGAAAACATCCCTCAAAA‑CCCCCCGCAC‑TTTTATCGCGGCCGCTCCGCCGTGGCCCCCATGTTGGACGGGACGACGCGCCATTGCCGTTA‑TTCCCACCCCA‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGcc                                                                                                                                             <  1:292551/248‑1 (MQ=255)
                                                                                                         ttCCGCC‑ATGGAATTAAAAAAGAAAGCCAAAAAGACATGAAGGACAACTCGAAAACAT‑CCCCAAAA‑CCCCCCCCCCTTTTTACCGCGGCCGCTTCGCCGTGGCCCCCATGTTGGACTGGCCGACGCCCCTTTGCCGTTT‑TTCCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCt                                                                                                                                            <  1:739854/251‑1 (MQ=255)
                                                                                                                catcgaattaaaaaaaaaaTCCAACAAGACATGAAGGACACCTCGTAACCAT‑CCCCAAAA‑CCCCCCCCCCTTTTTATCGCGGCCGCTTCGCCGTGCCCCCCTTGTTGGACTGGACGCCGCCCCATTGCCGTTA‑TTCCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCg                                                                                                                                      <  1:113101/235‑1 (MQ=255)
                                                                                                                       taaaagaaaAAATGAAACAAGACATGAAGGACACCTCGTAAACATGCCCCAAAC‑CCCCCCGCCT‑TTTTATCGCGGCCGCTTCGCCGTGCCACCCATGTTGGACTGGACGACGCCCCATTGCCGTTT‑TTCCCCCCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAAc                                                                                                                               <  1:714828/248‑1 (MQ=255)
                                                                                                                       taaaaaaaaaaaggcacaaaGACATGAAGAACACCTCGAAACCAT‑CCCCAAAACCCCCCCGCCT‑TTTTTTCGCGGCCGCTTCGCCGTGGCCCCCTTGTTGGACTGGCCGCCGCGCCTTTGCCTTTT‑TTCCCCCCGCC‑ATTTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAAc                                                                                                                               <  1:701765/233‑1 (MQ=255)
                                                                                                                               gaaATGCAACAAGACATGAAGGACAACTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGggcgg                                                                                                                        >  1:674335/1‑250 (MQ=255)
                                                                                                                                     caacaaGACATGAAGGACAACTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGa                                                                                                                  >  1:751002/1‑250 (MQ=255)
                                                                                                                                           gACATGAAGGACAACTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGc                                                                                                            >  1:694878/1‑250 (MQ=255)
                                                                                                                                            aCATGAAGGACAACTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGcc                                                                                                           >  1:188829/1‑250 (MQ=255)
                                                                                                                                            aCATGAAGGACAACTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGcc                                                                                                           >  1:429447/1‑250 (MQ=255)
                                                                                                                                                        aCTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTgg                                                                                                                                        >  1:732267/1‑209 (MQ=255)
                                                                                                                                                        aCTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACAtt                                                                                              >  1:636416/1‑251 (MQ=255)
                                                                                                                                                         cTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTTCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTg                                                                                             >  1:834931/1‑251 (MQ=255)
                                                                                                                                                         cTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTg                                                                                             >  1:742633/1‑251 (MQ=255)
                                                                                                                                                         cTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTg                                                                                             >  1:836358/1‑251 (MQ=255)
                                                                                                                                                         cTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTg                                                                                             >  1:387595/1‑251 (MQ=255)
                                                                                                                                                                                  ccGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACgggg                                                                     >  1:230337/1‑251 (MQ=255)
                                                                                                                                                                                       c‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCatcatc                                                                                                                                                                                         >  1:94832/1‑130 (MQ=255)
                                                                                                                                                                                       c‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTAt                                                                 >  1:504957/1‑250 (MQ=255)
                                                                                                                                                                                                                       aTGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGt                                  >  1:267790/1‑250 (MQ=255)
                                                                                                                                                                                                                       aTGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGt                                  >  1:418478/1‑250 (MQ=255)
                                                                                                                                                                                                                       aTGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGt                                  >  1:313842/1‑250 (MQ=255)
                                                                                                                                                                                                                       aTGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGt                                  >  1:745919/1‑250 (MQ=255)
                                                                                                                                                                                                                       aTGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGt                                  >  1:693958/1‑250 (MQ=255)
                                                                                                                                                                                                                            ggactggacGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGTCCGAt                             >  1:704222/1‑250 (MQ=255)
                                                                                                                                                                                                                            ggactggacGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGTCCGAt                             >  1:537/1‑250 (MQ=255)
                                                                                                                                                                                                                              acggcacgacgCCCTATTGCCTTTT‑TTCCCCCCGCC‑ATTTTCCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGTCCGATcg                           <  1:473865/245‑1 (MQ=255)
                                                                                                                                                                                                                                            cATTCCCGTTA‑TTCCCCCCACC‑AATTTCCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGTCCGATCGCGTGCAAAACGGCAt            <  1:120128/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    ttA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGTTCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGc                                                                    >  1:736538/1‑187 (MQ=255)
                                                                                                                                                                                                                                                       ttttCCCCCCCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGTCCGATCGCGTGCAAAACGGCATGTTCGGCGcc  <  1:680571/250‑1 (MQ=255)
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      
GCTGCCAGCCCGTGCTGATTTGGCTATTGATTTGCTGCCTGATTTCCTCCATTATTTCACGCCAATTT‑AATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCC‑ATCGGATTCAAGAAGAAATGCAACAAGACATGAAGGACAACTCGTAAACATGCCTCAAAA‑CCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCCGTTA‑TTTCCACCGCC‑AATTTTCCCGCCATGCCTTGCTTTACA‑CGGAAATGGTGGCGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGTCCGATCGCGTGCAAAACGGCATGTTCGGCGCC  >  1/247438‑247930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑