Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,275,683	0	T	G	50.0%	42.2 / 15.4	36	T1108P (ACC→CCC)	unknown	Chromosome partition protein MukB
Reads supporting (aligned to +/- strand):  ref base T (7/11);  new base G (18/0);  total (25/11)
Fisher's exact test for biased strand distribution p-value = 1.06e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTACGCAACGCACCAAGGGCTTTGTCCGACATGGAACGTAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTACTGTTGAAAGAACTTTGCAATTGAATCAACACTTGATTGTATTGTGCATATTGCGCTTGAACCTGACGCAATTGCTCGCGCTGTGTTTCGCGT  >  1/1275454‑1275933                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                            gTACGCAACGCACCAAGGGCTTTGTCCGACATGGAACGTAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑ATTTTTCGCTTTCCCTTCAATTGGGGGGGGGTTGTTTTTCATTGTAAg                                                                                                                                                                                                                                         >  1:28876/1‑251 (MQ=255) gTACGCAACGCACCAAGGGCTTTGTCCGACATGGAACGTAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑ATTTTTCGCTTCGCTTTCAA‑TGAGGGGGGGGTGTTTTTTAATGTAAGa                                                                                                                                                                                                                                        >  1:524729/1‑251 (MQ=255) gTACGCAACGCACCAAGGGCTTTGTCCGACATGGAACGTAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑AATTTTCGCTTCGCTTTTTA‑TTAGGGGGAGTTGTTTTTCTATGTaaaa                                                                                                                                                                                                                                        >  1:529489/1‑249 (MQ=255)  tACGCACCCCACCAAGGGCTTTGTCCGACATGGAACGTAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCCCCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGaa                                                                                                                                                                                                                                       <  1:398264/251‑1 (MQ=255)  tACGCAACGCACCAAGGGCTTTGTCCGACATGGAACGTAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGaa                                                                                                                                                                                                                                       <  1:335454/251‑1 (MQ=255)  tACGCAACGCACCAAGGGCTTTGTCCGACATGGAACGTAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGaa                                                                                                                                                                                                                                       <  1:94424/251‑1 (MQ=255)        acgcaCCAAGGGCTTTGTCCGACATGGAACGTAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑ATTTTTCGCTTCGCTTTCTA‑TGAGGGGGGGGTGTTTTTTAATGTAAAAAAGGCa                                                                                                                                                                                                                                  >  1:373038/1‑250 (MQ=255)           caCCAAGGGCTTTGTCCGACATGGAACGTAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGGAGAATTTTCC‑CCTCGCTTTTCA‑TGAGGGGGGGGTGTTTTTTAATGTAAAAAAGGCACTgg                                                                                                                                                                                                                              >  1:596137/1‑250 (MQ=255)             ccAAGGGCTTTGTCCGACATGGAACGTAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACg                                                                                                                                                                                                                            <  1:90286/251‑1 (MQ=255)                       tGTCCGACATGGAACGTAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCCCCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGaa                                                                                                                                                                                                                  <  1:321529/251‑1 (MQ=255)                                       tAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAAc                                                                                                                                                                                                   <  1:89390/250‑1 (MQ=255)                                          cTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTc                                                                                                                                                                                                 <  1:409917/249‑1 (MQ=255)                                                   ggATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTc                                                                                                                                                                                                 <  1:256919/240‑1 (MQ=255)                                                           atGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑ATTTTTCGCTTCGCTTTCAA‑TGAGGGGGAGTTGTTTTTCAATGTAAGAAAGGCACTGa                                                                                                                                                                                                                              >  1:642689/1‑203 (MQ=255)                                                                        acgaTTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCCCCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCgcgc                                                                                                                                                                 <  1:85185/251‑1 (MQ=255)                                                                                  cGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑ATTTTCCCCTTCGCTTTCCA‑TTGGGGGGAGTTGTTTTTCAATGTAAAAACGGCACCGACGATTAGTGGAAAGTTGTTGATAAAACTCATCCCCACGGATTTTTGCACGTTTTTCCGCGCCTTTGTCgg                                                                                                                                                        >  1:628760/1‑250 (MQ=255)                                                                                              tCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGGGGGGTGGTTTTTAATGTAAGAACGGCCCTGACGATTAGGGGAAAGTTGTTGTTACAACTCATCCCGACCGATTTTTTCACGTTCTTTCGCGCCTTCGTCGGCACGTACAcccca                                                                                                                                           >  1:410297/1‑249 (MQ=255)                                                                                              tCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑AATTTCCGCTTCGCTTTTAA‑TGAGGGGGGGTTGTTTTTTTATTTAAGAAAGGCACCGACGATTTGTGGGAAATTGTTTATACAAATCATCCCCACGGATTTTTTCACGGTCTTTCGCGCCTTCGTCGGCACGTACAcccca                                                                                                                                           >  1:135500/1‑249 (MQ=255)                                                                                                             aaaCGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCCTCGCTTTCAA‑TGAGGGGGAGGTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGGTGTTGATAAAACTCATCCCGACGGATTTTTGCACGGTCTTCCGCGCCTTCGTCCGCACGTACACCCAATTCGCCGAtttt                                                                                                                               >  1:538732/1‑247 (MQ=255)                                                                                                                                 gCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑AATTTTCGCCTCGCTTTCAA‑TGAGGGGGAGTTGTTTTTCAATGTAAGGACGGCACTGACGATTAGTGGGAAGTTGTTGCTACAAATCATTCCGACGGAATTTTTCACGTTCTTCCTCGCCTTCGTCGGGACGTACACCCCATTCGCCGATTTTCTGCATTAATTCCTGCCACA‑ttt                                                                                                       >  1:442510/1‑251 (MQ=255)                                                                                                                                    cATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGGGAGTTGTTTTTTAATGTAAGAACGGCACTGACGATTAGTGGGAAGTTGTTGATACAACTCATCCCGACGGATTTTTTCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGt                                                                                                    >  1:453211/1‑251 (MQ=255)                                                                                                                                    cATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑ATTTTTCCCCTCGCTTTCAA‑TTCGGGGGGGTTGTTTTTTAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTTTTACAAATCATTCCCACGGGTTTTTTCACGTTTTTTCGCCCCTTCGTCGGCACGTAACCCCCATTCGCCGAGTTTCTGcctttatttcttctaccttttgt                                                                                                     >  1:629425/1‑229 (MQ=255)                                                                                                                                                                 gggTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCTCTTTCAA‑TGAGGGTGAGTTGTTTTTTAATGAAAGAACGGCAACGGCGCTTAGTGGAAAGTTGTTGAGACAACTCCACCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCTTCGGCACGTACAACCAATTTGCCGCTTTTCTGCCTTAAATTCTGCAAAA‑TTTGGGTTTTACTGTTGTAAGAACTTTGCAATTGa                                                                       >  1:484545/1‑251 (MQ=255)                                                                                                                                                                 gggTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑ATTTTTTGCTTCGCTTTCAA‑TGAGGGGGAGGTGTTTTTTAATGTAAGAACGGCACTGACGATTAGTGGGAAGGTGTTGATACAACTCATCCCGACGGGTTTTTTTACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTTGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTACTGTTTAAAGAACTTTGCAATTGa                                                                       >  1:333078/1‑251 (MQ=255)                                                                                                                                                                      ttGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGGGAGGTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGGAAATTGTTGATACAACTCATCCCGACGGATTTTTTCACGTTCTTCCGCGCCCTCGTCGGCACGTACACCCCATTCGCCGATTTCCTGCATTAATTCCTGCAACATTTTGGTTTTT‑CTTTTGAAAGAACTTTTCAATTTAATCaa                                                                  >  1:626601/1‑251 (MQ=255)                                                                                                                                                                      ttGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑ATTTTCCGCTTCGCTTTTAA‑TGAGGGGGAGGTGTTTTTTAATGTAAGAACGGCACTGACGATTAGTGGAAAGGTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTTATTTCTGCAACA‑TTTGGGTTTTACTTTTGAAAGAACTTTTCAATTTAATCaa                                                                  >  1:510685/1‑251 (MQ=255)                                                                                                                                                                      ttGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑ATTTTCCGCTTCGCTTTTAA‑TGAGGGGGAGGTGTTTTTTAATGTAAGAACGGCACTGACGATTAGTGGAAAATTGTTGATACAACTCATCCCCACGGATTTTTTCACGTTCTTCCCCGCCTTCGTCGGCACGTACACCCAAATCGCCCATTTCCCGCATTAATTCCTGCAACA‑TTTGGGTTTTACTGTTGAAAGAACTTTGCAATTGAATCaa                                                                  >  1:399049/1‑251 (MQ=255)                                                                                                                                                                        gTAGTCCCGTTCCGCTTTACGAATGCGGCGGGGAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGGGGGGTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGGAAGGTGTTGATACAACTCATCCCGACGGATTTTTTCACGTTCTTCCGCGCCCTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTTCTGTTGAAAGAACTTTGCAATTGAATCAaca                                                                >  1:368457/1‑251 (MQ=255)                                                                                                                                                                                 ttCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTACTGTTGAAAGAACTTTGCAATTGAATCAACACTTGattgt                                                       <  1:59022/251‑1 (MQ=255)                                                                                                                                                                                        ttACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTACTGTTGAAAGAACTTTGCAATTGAATCAACACTTGATTGTATTGTGc                                                <  1:200348/251‑1 (MQ=255)                                                                                                                                                                                                             aTTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTACTGTTGAAAGAACTTTGCAATTGAATCAACACTTGATTGTATTGTGCATATTGCGCTTGAACCTGa                             >  1:277082/1‑250 (MQ=255)                                                                                                                                                                                                                          cttTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTACTGTTGAAAGAACTTTGCAATTGAATCAACACTTGATTGTATTGTGCATATTg                                          >  1:624104/1‑224 (MQ=255)                                                                                                                                                                                                                              cAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTACTGTTGAAAGAACTTTGCAATTGAATCAACACTTGATTGTATTGTGCATATTg                                          >  1:69226/1‑220 (MQ=255)                                                                                                                                                                                                                              cAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTACTGTTGAAAGAACTTTGCAATTGAATCAACACTTGATTGTATTGTGCATATTGCGCTTGAACCTGACGCAATTGCTcgcg               >  1:167740/1‑247 (MQ=255)                                                                                                                                                                                                                                   gAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTACTGTTGAAAGAACTTTGCAATTGAATCAACACTTGATTGTATTGTGCATATTg                                          >  1:287316/1‑216 (MQ=255)                                                                                                                                                                                                                                    aGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTACTGTTGAAAGAACTTTGCAATTGAATCAACACTTGATTGTATTGTGCATATTGCGCTTGAACCTGACGCAATTGCTCGCGCTGTGttt       >  1:426454/1‑250 (MQ=255)                                                                                                                                                                                                                                        tGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTACTGTTGAAAGAACTTTGCAATTGAATCAACACTTGATTGTATTGTGCATATTGCGCTTGAACCTGACGCAATTGCTCGCGCTGTGTTTCGCgt  <  1:98887/251‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                            GTACGCAACGCACCAAGGGCTTTGTCCGACATGGAACGTAGCTCATCGGCGGATAAATATGCCAATTCACGACGATTTAAGCGTTTTTCCACATCAGAATTGCGAGATAAACGCAGCACCACGCACCAGCTCATTTTAGCCGCCGTTACCAATTCGCGCTGGGTTTTGTAGTCCCGTTCCGCTTTACGAATGCGGCGGGTAAG‑ATTTTCCGCTTCGCTTTCAA‑TGAGGGTGAGTTGTTTTTCAATGTAAGAACGGCACTGACGATTAGTGGAAAGTTGTTGATACAACTCATCCCGACGGATTTTTGCACGTTCTTCCGCGCCTTCGTCGGCACGTACACCCAATTCGCCGATTTCCTGCATTAATTCCTGCAACA‑TTTGGTTTTTACTGTTGAAAGAACTTTGCAATTGAATCAACACTTGATTGTATTGTGCATATTGCGCTTGAACCTGACGCAATTGCTCGCGCTGTGTTTCGCGT  >  1/1275454‑1275933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑