Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,147,5160CT54.5% 19.3 / 48.5 33V139V (GTG→GTA) unknownABC‑type Fe3+ transport system, periplasmic component
Reads supporting (aligned to +/- strand):  ref base C (0/15);  new base T (18/0);  total (18/15)
Fisher's exact test for biased strand distribution p-value = 9.64e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-08
Rejected as consensus: Frequency below/above cutoff threshold.

AGTCAACGGCCGATGATGCCAGTAAGTCACCGGAAGCGGTATCATCCACGACCTGACCGGCGCCTTTACCGATTTTGGTTTTATTTTCTTTTAACTTTTCAAAGAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCGTATCGGCGT‑AAAAGAACCGTC‑GAAATCGGGAATCGGATTAATGATATTTTCTACTTC‑GGGCGAAATATAAGTTTCTAGCATGCC‑GGTTTCTTTCATATTGAAGAAGTCAGGGACTTCACTGGTCCAAATAACATCTGCCATAATCTGACCGGATTCTTTTTCCGTGGCAATTTTTGCCATGAGTTTGCCCGCACCGGCAGATTGATAG  >  1/1147275‑1147765
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                              
aGTCAACGGCCGATGATGCCAGTAAGTCACCGGAAGCGGTATCATCCACGACCTGACCGGCGCCTTTACCGATTTTGGTTTTATTTTCTTTTAACTTTTCAAAGAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTTTTTTTTTACAAAACgg                                                                                                                                                                                                                                                       >  1:49308/1‑251 (MQ=255)
 gTCAACGGCCGATGATGCCAGTAAGTCACCGGAAGCGGTATCATCCACGACCTGACCGGCGCCTTTACCGATTTTGGTTTTATTTTCTTTTAACTTTTCAAAGAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTTTTTTTTTACaaaaaggg                                                                                                                                                                                                                                                      >  1:230405/1‑246 (MQ=255)
                  ccAGTAAGTCACCGGAAGCGGTATCATCCACGACCTGACCGGCGCCTTTACCGATTTTGGTTTTATTTTCTTTTAACTTTTCAAAGAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TAcc                                                                                                                                                                                                                                     <  1:55317/250‑1 (MQ=255)
                  ccAGTAAGTCACCGGAAGCGGTATCATCCACGACCTGACCGGCGCCTTTACCGATTTTGGTTTTATTTTCTTTTAACTTTTCAAAGAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TAcc                                                                                                                                                                                                                                     <  1:630291/250‑1 (MQ=255)
                      tAAGTCACCGGAAGCGGTATCATCCACGACCTGACCGGCGCCTTTACCGATTTTGGTTTTATTTTCTTTTAACTTTTCAAAGAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAAtg                                                                                                                                                                                                                                <  1:43186/251‑1 (MQ=255)
                                gAAGCGGTATCATCCACGACCTGACCGGCGCCTTTACCGATTTTGGTTTTATTTTCTTTTAACTTTTCAAAGAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGc                                                                                                                                                                                                                       <  1:14321/250‑1 (MQ=255)
                                 aaGCGGTATCATCCACGACCTGACCGGCGCCTTTACCGATTTTGGTTTTATTTTCTTTTAACTTTTCAAAGAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCg                                                                                                                                                                                                                      <  1:249795/250‑1 (MQ=255)
                                                              ccTTTACCGATTTTGGTTTTATTTTCTTTTAACTTTTCAAAGAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTTTTTTTTTTCAAAAAGGGGGGTGTAGGGGG‑GAACTAATGTGGCTAAGAGTATCTGCGG‑AAAAAAAACGTC‑GAAAACggg                                                                                                                                                                                      >  1:510212/1‑251 (MQ=255)
                                                              ccTTTACCGATTTTGGTTTTATTTTCTTTTAACTTTTCAAAGAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCg                                                                                                                                                                                                                      <  1:509344/221‑1 (MQ=255)
                                                                             gTTTTATTTTCTTTTAACGTTTCAAAGAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGTGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGGAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCGTATCGGCGT‑AAAAGAACCGTT‑GAAATCGGGAATCGGATTAATGa                                                                                                                                                                        <  1:591233/250‑1 (MQ=255)
                                                                                                       gAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTTCAAAACGGGGGTTGTAGGCGA‑AACCTAATTTGCCTAAACGGATCTGCGT‑AAAAAAAACCTC‑GAAAAAGGGGATCGGGATAATGATAtttttttcttccggggacaaataag                                                                                                                                             >  1:242194/1‑230 (MQ=255)
                                                                                                         aTTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTACAAAAAGGGGGGTGTAGGGGAGAACCTAA‑GAGCCCAAGCGGATCGGCGG‑AAAAAAAACGTC‑GAAAAAGGGGAACGGATTAATGATATTTTTTTCTTC‑GGGGGAAATATAAGtt                                                                                                                                          >  1:256860/1‑251 (MQ=255)
                                                                                                                                            cTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTTCAAAACGGGGGGTGTAGGCGA‑TACCTAATGTGCCTAAGCGGATCGGCGG‑AAAAGAAACGGC‑GGAAACGGGGAACGGATTTATGATATTTTTTTCTTC‑GGGCGGAATATAAg                                                                                                                                            >  1:173131/1‑214 (MQ=255)
                                                                                                                                            cTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTACCAAACGGGGGTTGTAGGCGG‑TACCTAATGTGCCTAAGCGTATCGGCGT‑AAAAGAAACGTC‑GAAATCGGGGATCGGATTAATGATTTTTTTTTCTTC‑CGGCGGAAAATAAATTTCTAGCATGCC‑GGGTTCTTTTatatctttctcttt                                                                                                      >  1:372694/1‑241 (MQ=255)
                                                                                                                                            cTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCGTATCGGCGT‑AAAAGAACCGTC‑GAAATCGGGAATCGGATTAATGATATTTTCTACTTC‑GGGCGAAATATAAGTTTCTAGccc                                                                                                                                  <  1:164994/224‑3 (MQ=255)
                                                                                                                                            cTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCGTATCGGCGT‑AAAAGAACCGTC‑GAAATCGGGAATCGGATTAATGATATTTTCTACTTC‑GGGCGAAATATAAGTTTCTAGCATGCC‑GGTTTCTTTCATATTgaaga                                                                                                          <  1:7685/247‑1 (MQ=255)
                                                                                                                                              cATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTCCAAAACGGGGGTTGTAGGCGG‑GACCCAATGTGCCCTAGCGTATCGGCGT‑AAAAGAAACGGCGGAAACCGGG‑ATCGGGTTAATTATATTTTTTACTTC‑GGGCGGAAAATAAATTTCTAGCATGCC‑GGGTTTTTTTATATTTTAGAAGTCAg                                                                                                    >  1:384506/1‑251 (MQ=255)
                                                                                                                                              cATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTACAAAACGGGGGTTGTTGGGGG‑TACCTAATGTGCCTAAGCGTATCGGCGG‑AAAAAAAACGGC‑GGAAACGGGGATCGGATTAATGATATTTTTTTCTTT‑GGGCGGAATATAAGTTTCTTGCATGCC‑GGGTTTTTTCATATTGAAGAAGTCAg                                                                                                    >  1:510147/1‑251 (MQ=255)
                                                                                                                                              cATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTACAAAACGGGGGTTGTAGGCGG‑TACCTAATGTGGCTAAGCGGATCGGCGG‑AAAAGAAACGTC‑GAAAACGGGGAACGGATTAATGATATTTTTTTCTTC‑TGGCGGAATATAAGTTTCTTGCATGCC‑GGGTTTTTTCATATTTAAGAAGGCAg                                                                                                    >  1:153170/1‑251 (MQ=255)
                                                                                                                                                  tatGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTACAAAACGGGGGTTGTAGGGGG‑TACCCAATTTGCCTTAGCGTATCGGCGG‑AAAAAAAACGGC‑GAAAACGGGGATCGGGTTAATGATATTTTTTTCTTCTGGGGGAAA‑ATAAGTTTTTAGCATGCC‑GGTTTTTTTTATATTTTAGAAGTCAggggg                                                                                                >  1:52839/1‑249 (MQ=255)
                                                                                                                                                   atGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTTTTTTTTTACAAAACGGGTGTTGTAGGCGA‑TACCCAATGTGCCTAAGCGGATCGGCGTGAAAAAAACCG‑C‑GAAATCGGGGATCGGATTAATGATATTTTTTACTTC‑GGGGGAAATATAAGTTTCTAGCATGCC‑GGGTTCTTTTATAttttag                                                                                                           >  1:339307/1‑235 (MQ=255)
                                                                                                                                                        gTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTACAAAACGGGGGTTGTAGGGGG‑TAACTAATGTGCCTCAGCGGATCGGCGC‑AAAAGAAACGTA‑CAAATCGGGAATCGGGTTTATGATATTTTTTACTTC‑CGGCGGAATATAAGATTTTAGCATTCC‑GGGTTCTTTTTTATTTAAGAAATCAGGGACttta                                                                                            >  1:566428/1‑247 (MQ=255)
                                                                                                                                                        gTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCGTATCGGCGT‑AAAAGAACCGTC‑GAAATCGGGAATCGGATTAATGATATTTTCTACTTC‑GGGCGAAATATAAg                                                                                                                                            <  1:199352/202‑1 (MQ=255)
                                                                                                                                                                  gggCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTTCCAAAAGGGGGTTGTTGGGGA‑TACCTAAAGTGCCTAAGCGTATCGGCGT‑AAAAAAACCGGC‑GGAATCGGGGATCGGGTTAATGATATTTTTTTCTTC‑GGGCGGAAAATAAAGTTCTAGCATGCC‑GGTTTTTTTTATATTTAAGAAGTCAGGGAATTTACTgggcccaaaa                                                                                >  1:344520/1‑243 (MQ=255)
                                                                                                                                                                  gggCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTTCAAAACGGGGGTTGTTGGCGA‑TACCTTATGTGCCCTAGCGGATTGGCGG‑AAAAGAACCGGC‑GCAAACGGGGATCGGGTTAATTATAATTTTTTCTTT‑GGGGGGAAAATAAGATTTTAGCATTCCGGGTTTTTTTC‑TATTTAAGAAGTCAGGGGCTTTACTGGTCCAAATa                                                                                >  1:311220/1‑251 (MQ=255)
                                                                                                                                                                  gggCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCGTATCGGCGT‑AAAAGAACCGTC‑GAAATCGGGAATCGGATTAATGATATTTTCTACTTC‑GGGAGAAATATAAGTTTCTAGCATGCC‑GGTTTCTTTCATATTGAAGAAGTCAGGGAc                                                                                                <  1:282481/235‑1 (MQ=255)
                                                                                                                                                                        ggTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCGTATCGGCGT‑AAAAGAACCGTC‑GAAATCGGGAATCGGATTAATGATATTTTCTACTTC‑GGGCGAAATATAAGTTTCTAGCATGCC‑GGTTTCTTTCATATTGAAGAAGTCAGGGACTTCACTGGTCCAAATAACAt                                                                            <  1:231244/249‑1 (MQ=255)
                                                                                                                                                                                            ccTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCGTATCGGCGT‑AAAAGAACCGTC‑GAAATCGGGAATCGGATTAATGATAATTTCTACTTC‑GGGCGAAATATAAGTTTCTAGCATGCC‑GGTTTCTTTCATATTGAAGAAGTCAGGGACTTCACTGGTc                                                                                      <  1:284393/219‑1 (MQ=255)
                                                                                                                                                                                                   aTTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTACAAAACGGGGGTTGTAGGGGA‑TACCTAAAGTGCCTAAGCGTATCGGCGT‑AAAAAAAACGGC‑GAAAACGGGAAACGGATTAATGATATTTTTTACTTC‑GGGCGGAAAATAAGTTTTTAGCATGCC‑GGGTTTTTTTATATTTAAGAAGGCAGGGACTTTACTGGGCCAAAt                                                                                 >  1:93714/1‑217 (MQ=255)
                                                                                                                                                                                                    ttGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCGTATCGGCGT‑AAAAGAACCGTC‑GAAATCGGGAATCGGATTAATGATATTTTCTACTTC‑GGGCGAAATATAAGTTTCTAGCATGCC‑GGTTTCTTTCATATTGAAGAAGTCAGGGACTTCACTGGTCCAAATAACATCTGCCATAATCTGACCGGATTCTTTTTCCg                                              <  1:275755/251‑1 (MQ=255)
                                                                                                                                                                                                             aaGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTTACAAAACGGGGGTTGTTGGCGG‑TAACTAATGTGCCTTAGCGTATTGGCGT‑AAAAGAAACGTC‑GAAAACGGGGATCGGGTTAATTGTATTTTTTACTTT‑TGGCGGAATAAAAGTTTCTTGCATGCC‑GGGTTTTTTTATATTGAAGAAGTCAGGGAGTTCACTGGGCCAAATAACATCTGCCATAATTTGACCGGATTTTTTTTCCTTGGGAAttt                                     >  1:272261/1‑251 (MQ=255)
                                                                                                                                                                                                                      cGCCCATTGTGCCGGCGGTTCTTTTTTTACAAAACGGGGGGTTTAGGCGG‑TACCTAATGTGGCTAAGCg                                                                                                                                                                                                                      >  1:436682/1‑69 (MQ=255)
                                                                                                                                                                                                                                                tCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCGTATCGGCGT‑AAAAGAACCGTC‑GAAATCGGGAATCGGATTAATGATATTTTCTACTTC‑GGGCGAAATATAAGTTTCTAGCATGCC‑GGTTTCTTTCATATTGAAGAAGTCAGGGACTTCACTGGTCCAAATAACATCTGCCATAATCTGACCGGATTCTTTTTCCGTGGCAATTTTTGCCATGAGTTTGCCCGCACCGGCAGATTGATAg  <  1:13163/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                              
AGTCAACGGCCGATGATGCCAGTAAGTCACCGGAAGCGGTATCATCCACGACCTGACCGGCGCCTTTACCGATTTTGGTTTTATTTTCTTTTAACTTTTCAAAGAATTCCCAACCGAAAGTCGATTTCAATAATGACACACTCATATATGAAGTACCGGATAGGGCAGGGTTGGCAATACCGAATGCGCCTTTAAATTGCGGAGTAAGAATATCCGCCCATTGTGCCGGCGGTTCTTTTTTCACAAAGCGGGTGTTGTAGGCGA‑TACCTAATGTGCCTAAGCGTATCGGCGT‑AAAAGAACCGTC‑GAAATCGGGAATCGGATTAATGATATTTTCTACTTC‑GGGCGAAATATAAGTTTCTAGCATGCC‑GGTTTCTTTCATATTGAAGAAGTCAGGGACTTCACTGGTCCAAATAACATCTGCCATAATCTGACCGGATTCTTTTTCCGTGGCAATTTTTGCCATGAGTTTGCCCGCACCGGCAGATTGATAG  >  1/1147275‑1147765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑