Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,107,460	0	G	A	37.8%	68.6 / 15.5	45	N133N (AAC→AAT)	unknown	Long‑chain fatty acid transport protein
Reads supporting (aligned to +/- strand):  ref base G (8/20);  new base A (17/0);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.09e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTTTTTACATTGTCCTCACCCATAGAATTCAGTGCATTTTTGACGCCGGTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGCAGTTACAGGACCATTTATATGAATTCGCGAATCCACATAAACGCCACCGGCAGAAAATTCGTTTTGTTTGAACAGGCTCATTAATGCCGGGTTGGTGGCGACCACAGC  >  1/1107224‑1107705                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       cTTTTTACATTGTCCTCACCCATAGAATTCAGTGCATTTTTGACGCCGGTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTcc                                                                                                                                                                                                                                          <  1:50428/251‑1 (MQ=255) cTTTTTACATTGTCCTCACCCATAGAATTCAGTGCATTTTTGACGCCGGTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAAACCGTTTT‑A‑CCG‑CCAAAAAAACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTTAAACAAAATTAAAattcattc                                                                                                                                                                                                                                           >  1:415085/1‑250 (MQ=255)            gTCCTCACCCATAGAATTCAGTGCATTTTTGACGCCGGTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGGTAT‑A‑CCG‑CCCAAAATACCCGCATCATAAT‑CGCGGGAATATTCACT‑CTTTTAAACAAAATTAACATTCATTTCCCCACCCaaaa                                                                                                                                                                                                                               >  1:504859/1‑250 (MQ=255)                   cccATAGAATTCAGTGCATTTTTGACGCCGGTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCCAAAAAAACGGCATCATAAT‑CGCGGGGATATTCACT‑CTTTAAAACAAAATTAAAATTCATTCCCGCACCTAAAA‑TAAACtt                                                                                                                                                                                                                       >  1:167684/1‑251 (MQ=255)                      aTAGAATTCAGTGCATTTTTGACGCCGGTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAAAACGTTAT‑A‑CCC‑CCAAAAATAACGGCCTCATAAA‑CGCGGGGATATTTACT‑CTTTTAAACAAAATTAACATTTATTTCCCCCCCTAAAA‑TAAACTTAtt                                                                                                                                                                                                                    >  1:418517/1‑250 (MQ=255)                                gTGCATTTTTGACGCCGGTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAAc                                                                                                                                                                                                                         <  1:650596/236‑1 (MQ=255)                                gTGCATTTTTGACGCCGGTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATACGCTAT‑A‑CCC‑CCCAAAAAACCGGCCTCACAATACCCGGG‑ACATTCTCTCCTTAAAACCAAAG‑TAACATTCATTCCCGCACCCAAAA‑CAAACTTATCACTGATAggg                                                                                                                                                                                                          >  1:361287/1‑250 (MQ=255)                                               cGGTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACata                                                                                                                                                                                           <  1:222013/251‑1 (MQ=255)                                               cGGTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGGTAT‑A‑ACG‑CCAAAAAAAACCGCATCATAAA‑CGCGGGGATATTTACT‑CTTTTAAACAAAAATAAAATTTATTCCCGCACCCAAAG‑AAAAAATTTCAATTATAGGGGAGATaaaaaaaata                                                                                                                                                                                           >  1:282238/1‑245 (MQ=255)                                                ggTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAAACCGTTAT‑A‑CCC‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTTACT‑CTTTTAACCAAAATTAAAATTCATTCCCGCACCCAAAA‑AAAAATTATCATTTATAGGTGAGATAAAATAAatat                                                                                                                                                                                          >  1:195897/1‑251 (MQ=255)                                                 gTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCCAAAAAACCGGCATCATAAT‑CGCGGGGATATTTACT‑CTTTTAACCAAAATTAACATTCATTTCCCCCCCTAAAG‑TAAAATTATCATTGATAGGTGAGATAAAATACATAtt                                                                                                                                                                                         >  1:391906/1‑251 (MQ=255)                                                     tGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGGTAT‑A‑CCG‑CCAAAAAAACCCGCATCATAAA‑CGCGGGGATATTTACT‑CTTTTAACCAAAAATAAAATTCATTCCCCCCCCTCAAA‑TAAAATTATCATTGATAGGTGAGATAAAATACATATTTggg                                                                                                                                                                                     >  1:339332/1‑250 (MQ=255)                                                       gCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGgaa                                                                                                                                                                                    <  1:280577/250‑1 (MQ=255)                                                            aCCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGaatgaatg                                                                                                                                                                              <  1:456149/251‑1 (MQ=255)                                                                      cGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGaac                                                                                                                                                                    <  1:363992/251‑1 (MQ=255)                                                                        aTCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGAt                                                                                                                                                                                                              <  1:76563/207‑1 (MQ=255)                                                                        aTCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAAACCGTTAT‑A‑CCG‑CCAAAAAAACCGGCATCATAAA‑CGCGGGAAAATTTACT‑CTTTAAAACAAAAATAACAATCATTTCCGCACCCAAAA‑AAAAATTATCATTTATAGGTGAGATAAAAAACATATTTTGAATGAAAGCACCCGGaacaa                                                                                                                                                                  >  1:511673/1‑251 (MQ=255)                                                                                     ttCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTgg                                                                                                                                                                                      <  1:153826/218‑1 (MQ=255)                                                                                         cATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAAACCGTTAT‑A‑CCGCCCAAAAAAACC‑GCATCATAAT‑CGCGGGGATATTCACT‑CTTTTAAACAAAAATAACAATCATTTCCGCACCCCAAA‑TAAAATTATCATTGATAGGGGAGATAAAATAAATATTTGGAAAGAATGCACCCGGAACAAAGCTATGGGCATTaacg                                                                                                                                                 >  1:209081/1‑248 (MQ=255)                                                                                                  cATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATTCGTTAT‑A‑ACG‑CCAAAAAAACCGGCATTATAAT‑TGCGGGGATATTCACT‑CTTTTAAACAAAATTAACATTTATTTCCGCACCCAAAA‑AAAAATTATCATTTATAGGGGAGATAAAAAAAATATTTTGGATGAATGCACCTGGGACAAAGCTATGGGGATTTGCGCTTTCGGaa                                                                                                                                        >  1:234928/1‑251 (MQ=255)                                                                                                                ccgGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGAGAAATACGTTTT‑A‑CCT‑CCACAAATACCGGCAACAAAAA‑CGCGGGGAAATTCACT‑CTTTCAACCAAAATTGAAAATCATTACCGCCCCCAAAGATAAA‑TTTTTAATAATAGAGGAGAAAAAATACATATTTGTAATAAATTTACCCGTAACAACACTAAGGGCAATATCGCCTCCCGAAGAAACTTTTTGTc                                                                                                                           >  1:588024/1‑250 (MQ=255)                                                                                                                          aaTTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCCAAAATACCGGCATCATAAT‑CGCGGGAAAATTTACT‑CTTTAAACCAAAATTAACATTCATTCCCGCACCTAAAG‑TAAAATTATCATTGATAGGTGAGATAAAATACATATTTGgaa                                                                                                                                                                                    >  1:449519/1‑183 (MQ=255)                                                                                                                            ttattaGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGc                                                                                                              <  1:349552/251‑1 (MQ=255)                                                                                                                            ttattaGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGc                                                                                                              <  1:197889/251‑1 (MQ=255)                                                                                                                                taGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGaac                                                                                                                                                                    <  1:337312/193‑1 (MQ=255)                                                                                                                                  gTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑ACCCC‑CCAAAAAAACC‑GCATCATAAT‑CGCGGGGATATTTACT‑CTTTTAACCAAAGTTAACATTCATTCCCCCACCTAAAA‑TAAACTTATCATTGATAGGGGAGATAAAATACATATTTTGAATGAATGCACCCGGAACAACGCTATGGGCATTAGCGCTTCCGGGAGCAACTTTTTTTCCTCTGATATaaac                                                                                                              >  1:169433/1‑243 (MQ=255)                                                                                                                                     aTACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGCAGTTACAgg                                                                                                     <  1:644683/251‑1 (MQ=255)                                                                                                                                        cGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑ACG‑CCAAAAAAACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAAACAAAGTTAACATTCATTTCCGCACCTAAAG‑TAAAATTATCATTGATAGGGGAGATAAAATACATATTTTGAATGAATGCACCTGGAACAAAGCTATTGGCATTAGCGCTTCCGGAAGCAACTTTTTTTCCTCTGATATAAACAGTTACAGGaa                                                                                                   >  1:554044/1‑249 (MQ=255)                                                                                                                                        cGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCCTTATAA‑CCC‑CCAAAAA‑ACCGGCATCATAAT‑CGCGGGGATATTCACT‑CTTTTAAACAAAATTAACATTCATTCCCCCACCCAAAA‑TAAAACTATCCTTTTTAGGGGAGATAAAATACATATTTTGAATGAAAGCACCCGGAACAACACTATGGGCATTAGCGCTTCCGGAAACAACTTTTTTTCCTCTGATATAAACAGTTATAGGAc                                                                                                   >  1:636097/1‑250 (MQ=255)                                                                                                                                        cGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAAACCGTTAT‑A‑CCC‑CCCAAAATACCGGCATCATAAA‑CCCGGGAAAATTCACT‑CTTTAAACCAAAGATAACATTCATTCCCGCACCTAAAA‑AAAACTTATCATTTATAGGGGAGATAAAATACATAtt                                                                                                                                                                                         >  1:300516/1‑164 (MQ=255)                                                                                                                                         ggTATGACCCACTAAGATTCAGACTAATGGCGGTTAAAACCGTTAT‑A‑CCG‑CCCAAAAAACCCGCATCATAAT‑AGCGGGGATATTTACT‑CTTTAAACCAAAATTAACATTCATTCCCGCACCCAAAG‑AAAACTTTTCATTTATAGGGGAGATAAAATACAAATTTTGAATGAATGCACCTGGAACAACGCTATTGGCATTAGCGCTTCCGGAAGCAACTTTTTTTCCTCTGATATAAACAGTTAAAGGACCa                                                                                                 >  1:267854/1‑251 (MQ=255)                                                                                                                                          gTATGACCCACTAAGATTCAGACTAATGGCGGTTTAATTCTCTAT‑ACCCC‑CCAAAAAAACCGG‑ATCATAAA‑CGACGGAATATTCACT‑CTTTCAACCAAAATTAAAATTCCTTCCCGCACCCAAAG‑TAAACTTTTCATTGATAAGGGAGGAAAAATAAAAATTTTGGATTAATTTACCCGGAACAACGCTATCGGCCTTAAGGCTTTCGGGAGCAACCTTTTGTTCTCTtataataacaagtaaacgaccat                                                                                                >  1:591563/1‑232 (MQ=255)                                                                                                                                                 ccACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGCAGTTACAGGACCATTTATATg                                                                                         <  1:129972/251‑1 (MQ=255)                                                                                                                                                 ccACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGCAGTTACAGGACCATTTATATg                                                                                         <  1:327391/251‑1 (MQ=255)                                                                                                                                                   aCTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATAc                                                                                                                                                                                              <  1:482389/148‑1 (MQ=255)                                                                                                                                                    cTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCC‑CCAAAAATACCGGCCTCATAAT‑CGCGGGGATATTCACT‑CTTTAAAACAAAAGTAAAATTCATTCCCGCACCTAAAG‑AAAACTTATCATTTATAGGTg                                                                                                                                                                                                         >  1:596560/1‑136 (MQ=255)                                                                                                                                                                                                       ccGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTg                                                                                                                             <  1:605828/164‑1 (MQ=255)                                                                                                                                                                                                           catcatAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGCAGTTACAGGACCATTTATATGAATTCGCGAATCCACATAAACGCCACCGGCAGAAAATTCGTTTTGTTTGAACAgg                                  >  1:29716/1‑251 (MQ=255)                                                                                                                                                                                                               atAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGCAGTTACAGGACCATTTATATGAATTCGCGAATCCACATAAACGCCACCGGCAGAAAATTCGTTTTGTTTGAACAGGCTCa                              >  1:529464/1‑251 (MQ=255)                                                                                                                                                                                                                       cgGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGCAGTTACAGGACCATTTATATGAATTCGCGAATCCACATAAACGCCACCGGCAGAAAATTCGTTTTGTTTGAACa                                    <  1:643255/238‑1 (MQ=255)                                                                                                                                                                                                                       cgGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGCAGTTACAGGACCATTTATATGAATTCGCGAATCCACATAAACGCCACCGGCAGAAAATTCGTTTTGTTTGAACAGGCTCATTAATGc                       <  1:421434/251‑1 (MQ=255)                                                                                                                                                                                                                             tatTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGAAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGCAGTTACAGGACCATTTATATGAATTCGCGAATCCACATAAACGCCACCGGCAGAAAATTCGTTTTGTTTGAACAGGCTCATTAATGCCGGGtt                 >  1:420734/1‑251 (MQ=255)                                                                                                                                                                                                                                      ctTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGAt                                                                                                                                                                                                              <  1:500441/54‑1 (MQ=255)                                                                                                                                                                                                                                      ctTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGCAGTTACAGGACCATTTatat                                                                                          >  1:57231/1‑170 (MQ=255)                                                                                                                                                                                                                                             ccAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGCAGTTACAGGACCATTTATATGAATTCGCGAATCCACATAAACGCCACCGGCAGAAAATTCGTTTTGTTTGAACAGGCTCATTAATGCCGGGTTGGTGGCGACCAcagc  <  1:104034/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       CTTTTTACATTGTCCTCACCCATAGAATTCAGTGCATTTTTGACGCCGGTAATGGCGATACCGGCAGTGCGATCAAGTTCTGCTTTCGCATAGACACCATTTAAACCGAAACCGGCAGTTAAATTATTAGTAATACGGTATGACCCACTAAGATTCAGACTAATGGCGGTTAAATCCGTTAT‑A‑CCG‑CCAAAAATACCGGCATCATAAT‑CGCGGGAATATTCACT‑CTTTAAACCAAAGTTAACATTCATTCCCGCACCTAAAG‑TAAACTTATCATTGATAGGTGAGATAAAATACATATTTGGAATGAATGCACCTGGAACAACGCTATGGGCATTAGCGCTTCCGGAAGCAACTTTTTGTCCTCTGATATAAGCAGTTACAGGACCATTTATATGAATTCGCGAATCCACATAAACGCCACCGGCAGAAAATTCGTTTTGTTTGAACAGGCTCATTAATGCCGGGTTGGTGGCGACCACAGC  >  1/1107224‑1107705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑